0 оценок0% нашли этот документ полезным (0 голосов)
27 просмотров3 страницы
50 spots were found to be differentially expressed on induced and uninduced MPRO cell lines of which 28 were identified using MS and correlated to pI and molecular weight. Found 188 proteomic, nonredundant tyrosine LC-MS / MS phosphorylated sites of which 77 are novel. Further investigations of the protein expression signature of CML cells are warranted to allow the development of additional molecular targeted therapeutic agents.
50 spots were found to be differentially expressed on induced and uninduced MPRO cell lines of which 28 were identified using MS and correlated to pI and molecular weight. Found 188 proteomic, nonredundant tyrosine LC-MS / MS phosphorylated sites of which 77 are novel. Further investigations of the protein expression signature of CML cells are warranted to allow the development of additional molecular targeted therapeutic agents.
Авторское право:
Attribution Non-Commercial (BY-NC)
Доступные форматы
Скачайте в формате DOC, PDF, TXT или читайте онлайн в Scribd
50 spots were found to be differentially expressed on induced and uninduced MPRO cell lines of which 28 were identified using MS and correlated to pI and molecular weight. Found 188 proteomic, nonredundant tyrosine LC-MS / MS phosphorylated sites of which 77 are novel. Further investigations of the protein expression signature of CML cells are warranted to allow the development of additional molecular targeted therapeutic agents.
Авторское право:
Attribution Non-Commercial (BY-NC)
Доступные форматы
Скачайте в формате DOC, PDF, TXT или читайте онлайн в Scribd
Strategy 50 spots were found to be differentially expressed on induced and uninduced MPRO cell lines of which 28 were identified using MS and correlated to pI and Molecular weight these were GRP78, Lian, Z. et MPRO 2D- PAGE Cytoplasmic Gamma Actin, al (2001) (murine BCR- and MS, Proliferating cell nuclear antigen, (28) ABL cell line) MS/MS APS kinase, Pyrubate kinase 3, Melanoma X-actin, Glyceraldehyde-3-phosphate dehydrogenase, Stefin 3, Guanine nucleotide binding protein, Triosephosphate isomerase, Testis-derived c-abl protein, RNA binding motif protein etc. Compared cell lines pre and post K562, KU812, imatinib treatment for tyrosine Goss, V. et Phospho SUP- phosphorylated sites. Found 188 al (2006) proteomic, B15,BV173,K nonredundant tyrosine (29) LC-MS/MS CL22,SD1 phosphorylated sites of which 77 are novel. 2D-PAGE was carried from pH 3-11. 84 protein spots were different totaling 44 unique proteins. They further classified the proteins as 1. Heat Shock CML cell lines Proteins and Chaperones 2. LAMA 84 S Nucleic Acid Binding/ Ferrari, G. (imatinib Synthesis/Stability. 3. Structural 2D-PAGE, et al (2006) sensitive) and Proteins. 4. Cell Signaling. 5. MS (30) LAMA 34 R Metabolic Enzymes. They (imatinib believe that further investigations resistant) of the protein expression signature of CML cells are warranted to allow the development of additional molecular targeted therapeutic agents. Balabanov, K562 2D-PAGE, Investigated the effects of S. et al MS imatinib on the protein (2007) (31) expression profiles of BCR- ABL positive cells. Found eIF5A a site of hypusination representing the transfer of an amino-butyl residue to lysine to be down regulated in imatinib sensitive cells. Identified inhibition of eIF5Ahypusination as a promising new approach for combination therapy in BCR- ABL positive leukemias Hantschel, Chemical Found Tec kinases Btk and Tec O. et al K562 Proteomics, are prominent targets for (2007) (32) LC-MS/MS dasatinib inhibition. Compared CML and APL cell lines for differences in plasma Lee, S. J. et K562 and LC- membrane proteins by al (2007) AML cell line MS/MS concentrating these using a (33) NB4 biotin- avidin affinity purification system 2D PAGE analysis of proteins in cells was performed according to O’Farrel (14), with a slight modification. In brief,cells (2 3 107) were solubilized in 400 ml of lysis buffer containing 9.5 M Nunoi, H et Human urea, 2% NP40, 2% Pharmalyte al (1999) 2D-PAGE Neutrophils (1.6% pH 5–8 and 0.4% pH 3– (34) 10), and 5% mercaptoethanol. Isoelectric focusing was performed at 500 V for 24 hours followed by the 2D SDS PAGE. Proteins on the gel were stained with 2D Silver Stain Identified 191 protein spots corresponding to 142 different proteins of which 63% were cancer-related proteins. Detailed analysis of 35 differentially Fontana, S CML cell lines 2D-PAGE/ expressed proteins revealed that et al (2007) LAMA84, MS LAMA84 cells preferentially (35) K562, KCL22 expressed proteins associated with an invasive behavior, while K562 and KCL22 cells preferentially expressed proteins involved in drug resistance. Govekar, R. Tumors of the 2D-PAGE/ Cancer proteomic research et al (2009) Gingivo- MS-MSMS conducted in India. 40 ug of (36) buccal protein was used for 2D-PAGE complex on pH 4-7 strips (Bio-Rad) followed by silver staining. Spots were compared using Mann- Whitney’s test and those with significant differences in expression and/or with tumor to normal ratios of 2 and above or 0.5 and below were taken as ‘differentiators’