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Course Description: Biol 210 is a 5 unit college transferable course that introduces you to the
microbial world…a world of viruses, bacteria, molds, yeasts, protozoa, algae, and helminths.
Emphasis will be placed on microbial structure, metabolic activities, microbial genetics, host-
parasite relationships, host resistance, and the control of microbes. Lab activities will include
isolation and cultivation of bacteria, studies of bacterial isolates, understanding bacterial population
dynamics, control of microbial growth, mycology, parasitology, and virology.
Required Lab Manual: Laboratory Theory and Application, 2nd or 3rd Ed. by Leboffe and Pierce
Grading: Your course grade is based on a percent of accumulated points. These points are
acquired by examinations, quizzes, lab assignments, and lab reports. The grading scale is:
Make-up exams: Only one make-up lecture exam will be allowed during the semester. Based on an
excused absence…you must contact me the day of the exam. Make-ups may be essay exams.
Note: You must take all lab practical exams during the time/day it is given, there is no way to
“make-up” a lab practical exam on days they are not given. There are no make-up quizzes in
lecture or lab. If you miss a quiz it will count as dropped quiz.
Academic dishonesty on exams or quizzes will result in a score of zero for that exam or quiz and
could be cause for an instructor drop (See the campus policy regarding academic dishonesty). Do
not give or accept help during an exam!
Bio210 – Spring 2011 (Revised 1/27/2011)
Lecture has a total of 600 points which come from 4 exams, 5 quizzes (lowest quiz score dropped),
and 1 written assignment.
Points for lab total 400 and include 2 midterm exams, a final exam, 5 quizzes (lowest quiz score
dropped), assignments, and an unknown project. Late lab assignments may not be accepted or will
be given a penalty depending on the time frame of the assignment.
Please note that all quizzes (lecture and lab) are given at the beginning of the period and take about
20 minutes. If you are late, you will not be given extra time to complete your quiz. There are no
make-up quizzes.
Note: Orange Coast College policy on attendance states: "...a student who has been absent for 3
consecutive class meetings in a given section is to be dropped by the instructor.”
Tutoring for many different programs including Microbiology and English Language are
available in the Student Success Center located directly across from the microbiology teaching lab
(C&L 108).
Any student who feels he/she may need an accommodation based on the impact of a learning
disability or other disability should contact Disabled Students Programs and Services (DSP&S) in
the Special Services Building to schedule an appointment.
1 T Feb 1 Introduction 1
R Feb 3 History & Nomenclature 1
2 T Feb 8 Chemistry 2
R Feb 10 Chemistry 2
3 T Feb 15 Lecture Quiz 1; Prokaryotes & Eukaryotes 4
R Feb 17 Prokaryotes & Eukaryotes 4
4 T Feb 22 Microbial Metabolism 5
R Feb 24 LECTURE EXAM 1 1, 2, 4
Sa Feb 26 ****Last Day to Withdraw to Avoid a “W” ****
5 T Mar 1 Microbial Metabolism 5
R Mar 3 Microbial Metabolism 5
6 T Mar 8 Lecture Quiz 2; Microbial Growth 6
R Mar 10 Control and Antimicrobial Drugs 7, 20
7 T Mar 15 Control and Antimicrobial Drugs 7, 20
R Mar 17 Lecture Quiz 3; Control and Antimicrobial Drugs 7, 20
8 T Mar 22 Microbial Genetics 8
R Mar 24 LECTURE EXAM 2 5, 6, 7, 20
T Mar 29 Spring Break, Campus Closed
R Mar 31 Spring Break, Campus Closed
9 T Apr 5 Microbial Genetics 8
R Apr 7 Microbial Genetics 8
10 T Apr 12 Eukaryotes: Fungi, Algae, and Protozoa 12
R Apr 14 Eukaryotes: Fungi, Algae, and Protozoa 12
11 T Apr 19 Lecture Quiz 4; Eukaryotes: Fungi, Algae, and Protozoa 12
R Apr 21 Virology 13
12 T Apr 26 Virology 13
R Apr 28 Virology 13
Sa Apr 30 ****Last Day to Drop****
13 T May 3 Microbial Pathogenesis 15
R May 5 LECTURE EXAM 3 8, 12, 13
14 T May 10 Microbial Pathogenesis 15
R May 12 Microbial Pathogenesis 15
15 T May 17 Lecture Quiz 5; Immunology 16, 17
R May 19 Immunology / Vaccines 17, 18
16 T May 24 Immunology / Vaccines 17, 18
R May 26 LECTURE EXAM 4 15, 16, 17, 18
2 M/T Feb 7/8 Isolation of bacteria (Part I): Streak plate #1 1-4
Negative Stain; Gram stain 3-6; 3-7
W/R Feb 9/10 Check Streak Plate #1 and set up Streak Plate #2 1-4
Yeast and filamentous fungi: Tape mounts; Slide cultures (demo ) 12-1; 12-2
Check and discuss environmental plates (Observe - Fungi) 2-1; 2-2
3 M/T Feb 14/15 Quiz 1; Isolation of bacteria (Part II): Standard plate count 6-1
Endospore stain; Check Streak plate #2 3-10
W/R Feb 16/17 OF-glucose; PR-broth; Capsule stain; Acid-fast stain 5-2; 5-3; 3-9; 3-8
Read results for Standard plate count
5 M/T Feb 28/1 Quiz 2; Read results for IMViC, Starch hydrolysis, and ONPG
W/R Mar 2/3 Lab Practical Exam #1
Protein Catabolism
6 M/T Mar 7/8 Decarboxylase; Phenylalanine; Urease; Casease 5-10; 5-11; 5-13; 5-14
TSI (H2S only) 5-21 (and 5-20)
W/R Mar 9/10 Gelatinase; Motility/Indole/Ornithine (MIO - similar to SIM) 5-15; 5-20 & handout
Read results for Phenylalanine, Urease, Casease, and TSI (HSS only)
Respiration
7 M/T Mar 14/15 Anaerobic respiration: Anaerobic jar, Nitrate reduction 2-8, 5-7
Aerobic respiration: Catalase, Oxidase 5-5, 5-6
Read results for Decarboxylase, Gelatinase, MIO
W/R Mar 16/17 Set up Temperature exp.; pH demo; Osmotic pressure demo 2-9; 2-10; 2-11
Thioglycollate broth demo 2-7
Read results for the Anaerobic jar and Nitrate reduction
*Remember antiseptics/disinfectants for next class
10 M/T Apr 11/12 Quiz 4; Read results for Epidemiology and BGLB tubes 7-6; 8-13
Review
W/R Apr 13/14 Lab Practical Exam #2
14 M/T May 9/10 Quiz 5; Blood smear differentials; Blood typing 11-1; 11-5
View Helminth slides 12-4
W/R May 11/12 Finish Helminth slides 12-4
16 M/T May 23/24 Clean up the lab; Finish all lecture material
W/R May 25/26 No Lab; Lecture Final Exam
1. Do not come to lab late, new procedures (which often involve safety) are discussed at the beginning of lab.
2. Wear a lab coat during all experimental procedures in the lab. Store your coat on the coat rack or in an
assigned drawer.
3. Be aware of Bunsen burner flames. Turn off burners when not in use. Secure long hair to prevent accidental
exposure to open flames. Report burns and other injuries to your lab instructor immediately.
4. Use the eye wash station immediately, should any reagent accidentally get into your eyes.
6. Place contaminated items in autoclavable containers for disposal. Do not mix glass with plastic items.
7. Use the biohazard bag to dispose of biological waste…DO NOT use the trash basket to dispose of biological
waste.
8. Remove old cultures from the refrigerator or incubator and place them in the biohazard bag for disposal.
Remove labels from glassware when disposing. Do not remove cultures from the lab or place them in your
lab drawer.
9. Report accidental spills of cultures to your instructor immediately. Do not attempt to pick up broken glass
yourself.
10. Try to avoid bringing your hands to your mouth while in lab.
11. Wash your hands with soap and water before leaving lab.
14. Inform your lab instructor if you are pregnant or if you are immunosuppressed as some lab exercises may
pose unique problems in such cases.
15. Wear goggles when using reagents and when near ultraviolet lights.