INTERNATIONAL ISO STANDARD 5667-3 Fifth edition 2018-05 Water quality — Sampling — Part 3: Preservation and handling of water samples Qualité de eau — Echantillonnage — Partie 3: Conservation et manipulation des échantillons d'eau Reference number 180 5667-3:2018(E) Ete documento ha ado agi {CION HIDROGRAFICA DEL EBRO a 2019-2, © 180 2018, ra poer tz en un tema de eaten deers poner dela corresposientenia de AENORISO 5667-3:2018(E) A COPYRIGHT PROTECTED DOCUMENT © 1802018 All rights reserved. Unless otherwise specified, or required in the context ofits implementation, no part ofthis publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting ‘on the internet or an intranet, without prior written permission. Permission can be requested from either 180 at the address below or 1S0's member body in the country ofthe requester. 180 copyright office CP 401» Ch, de Blandonnet 8 (CH-1214 Vernier, Geneva Phone: +41 227490111 Fax: +41 22 74909 47 Email: copyright@iso.org Website: wwiso.org Published in Switzerland u te documento ha sido adgudo por CONFEDERACION HIDROGRAFICA Det EARATWLZIA,- All rights reserved ara poder silo enum stems dered tern, debers pon dea oreo zea de AENORISO 5667-3:2018(E) Contents Page Foreword. 7 " e Eee iv Introduction. : : = v to-- Scopes iH 7 ef7eessanananarn 1 2 Normative references..... srasaneatet 7 a 3 Terms and definitions... oe a, 4 Sampling and chain of custody... -sonenenn a es 2 5 Reagents and materials. eee te eee. 6 Containers... sae as ae a5 61 Container selection and preparation ee 5 62 Filtration on site... a pairs eee 5 63° Filling the containe? cso i pias 7 Sample handling and preservation ....-c0.0- reo one : 5 7.1 Sample handling and preservation for physical and chemical examination a5 7.2 Sample handling and preservation for biological examinatiON wumcwnnnnnn 6 7.3 Sample handling and preservation for radiochemical analysis as -7 8 Sample transport........ = Be ee <_esennaneGRnORES 7 9 Identification of samples é cae . = 8 10 Sample reception aoe : — oon 11 Sample storage... - 7 = 8 Annex A (informative) Techniques for sample preservation... i . 40 Annex B (informative) Container preparation c.eccmesnncnenenn seen Annex C (informative) Protocol as used in Dutch validation studies. tahaesatet cds 47 Bibliography scssipireete! ‘© 1SO 2018 ~ All righitsibeSPEEtha silo adquirdo por CONFEDERACION HIDROGRAFICA DEL EBRO el 2019-21 if ara pderulizaro en un tera deed interno deer pons dea corepodien era de AENORISO 5667-3:2018(E) Foreword {SO (the International Organization for Standardization) isa worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work 180 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso,org/directives). Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the 1S0 list of patent declarations received (see www.iso.0rg/patents). Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement. For an explanation on the voluntary nature of standards, the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISO's adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: This document was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 6, Sampling (general methods). ‘This fifth edition cancels and replaces the fourth edition (ISO 5667-3:2012), of which it constitutes a minor revision. The changes compared to the previous edition are as follows — updated references in Table A.1; — clarification in the Introduction concerning use of the preservation times and conditions set out in Alist ofall parts in the ISO 5667 series can be found on the ISO website, v Exe docimenta ha sdo adquiido por CONFEDERACION HIDROGRAFICA DEL EMRITLAOIA,” All rights reserved ara poer lal nun ite eee debe ions dee eset ec de ABNOR,Introduction This document is intended to be used in conjunction with ISO 5667-1, which deals with the design of sampling programmes and sampling techniques Where possible this document has been brought into line with current standards, Where new research or validation results have provided new insights, the latest knowledge has been used. Guidance on validation protocols can be found in ISO 17034. Oro? 3 provides in Iable A.1 validated preservation times and/or conditions as well as descriptions of best practice. Table A.t also refers, for each analyte, to those ISO standards available at the dave ct Publication ofthis ISO 5667-3. This is however not an exhaustive list. Other methods may be used when they have been validated. However, itis strongly recommended that where a method validation te not available, the preservation times for the analyte as listed in Table A. for ISO test methods be followed Tite Preservation and storage conditions and maximum storage times per analyte as listed in Table A.1 should be regarded as default conditions to be applied in the absence of any other informaticn, However, if validation of preservation techniques and holding times has been carried out, relative pecific circ ae and matrices, by a laboratory, then, provided that ee ae of this validation where they differ from those set out in Table A.1 of this standard, these validated Attention is drawn to the proposed development of a new part in the ISO 5667 series, which further elaborates on 1S0 5667-3:2018, Annex C, and which will contain guidelines and the elaboration of ihe cow (© 1502010 - All lgbtsgeSAiAtAy ste agai por CONFEDERACION HIDROGRAFICA DEL EBRO el s9-2 ara pserwlizaso eau sem: de redintero, brs ipo dea corepudene bene te ABNORfate documento do adguio pot CONFEDERACION HIDROGRAFICA DEL EBRO e209: rape uuario en un ena dee nero deer caper de correspon etn de AENORINTERNATIONAL STANDARD ISO 5667-3:2018(E) Water quality — Sampling — Part 3: Preservation and handling of water samples NOTICE — This document and the analytical International Standards listed in Annex A are sempigmentary. Where no analytical International Standard is applicable, the technique(s) described in Tables A.J to A.3 take(s) normative status. 1 Scope ‘This document specifies general requirements for sampling, preservation, handling, transport and Storage of all water samples including those for biological analyses, {is not applicable to water samples intended for microbiological analyses as specified in ISO 19458, Scotoxicological assays, biological assays and passive sampling as specified inthe scope of 180 Sep oe ‘This document is particularly appropriate when spot or composite samples cannot be analysed on site and have to be transported to a laboratory for analysis, 2 Normative references The following documents are referred to in the text in such a way that some or all of their content cousatytes requirements of this document. For dated references, only the edition cite applies. For Lndated references, the latest edition ofthe referenced document (including any amendments) applies. 180 3696, Water for analytical laboratory use — Specification and test methods 180 5667 (all parts), Water quality — Sampling 180 19458, Water quality — Sampling for microbiological analysis 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 'S0 and IEC maintain terminological databases for use in standardization at the following addresses: — TEC Electropedia: available at hrtp.//www.electropedia.org/ — 180 Online browsing platform: available at https://wwwiso.org /obp 34 integrity Tegel ae aay ene berameter(s) of interest, information or content of the sample container has not been altered or lost in an unauthorized manner or subject to loss of representativees (0180 2018 - Al ie eaeeHths co sda por ONFEDERACION HIDROGRAFICA DEL e8ROsta19 2 7 poser lean we stead re iter, eb iene dea corespatenslsnen de eoNOR32 sample preservation any procedure used to stabilize a sample in such a way that the properties under examination are maintained stable from the collection step until preparation for analysis, Note 1 to entry: Different analytes may require several samples from the same source that are stabilized by different procedures, [SOURCE: ISO 11074:2015, 4.4.20, modified — Note 1 to entry has been added] 3.3 sample storage process, and the result of keeping a sample available under predefined conditions, usually fora specified time interval between collection and further treatment of a sample Note 1 to entry: Specified time is the maximum time interval. [SOURCE: ISO 11074:2015, 4.4.22, modified — Note 1 to entry has been added; “soil sample” has been changed to “sample” 34 storage time period of time between filling of the sample container and further treatment of the sample in the laboratory, if stored under predefined conditions Note 1 to entry: Sampling finishes as soon as the sample container has been filled with the sample, Storage time ends when the sample is taken by the analyst to start sample preparation prior to analysis. Note 2to entry: Further treatment is, for most analytes, a solvent extraction or acid destruction. The initial steps of sample preparation can be steps complementary to the storage conditions for the maintenance of analyte concentrations 4 Sampling and chain of custody If there is a need to take samples, this is done according to a sampling programme. The first step is to design a sampling programme. Guidance on this topic is given in ISO 56674. Depending on the sample type and matrix, the guidelines found in ISO 19458 and in the relevant part(s) ‘of ISO 5667 shall be consulted. ‘The process of preservation and handling of water samples consists of several steps. During this process, the responsibility for the samples might change. To ensure the integrity of the samples, all steps involving the sample shall be documented. All preparation procedures shall be checked to ensure positive or negative interferences do not occur. ‘Asa minimum, this shall include the analysis of blanks (eg. field blank or sample container) or samples containing known levels of relevant analytes as specified in ISO 5667-14. 5 Reagents and materials WARNING — Certain preservatives (e.g. acids, alkalis, formaldehyde) need to be used with caution. Sampling personnel should be warned of potential dangers, and appropriate safety procedures should be followed. ‘The following reagents are used for the sample preservation and shall only be prepared according to individual sampling requirements. All reagents used shall be of at least analytical reagent grade and water shall be of at least ISO 3696, grade 2. Acids referred to in this document are commercially available “concentrated” acids. 2 {te docameato ha sid adguirdo por CONFEDERACION HIDROGRAFICA Det EARASOLAAIA,- All rights reserved Pan por aco en a tra de eterna, debe poner de conespodiente Leesa de AENORISO 5667-3:2018(E) All reagents shall be labelled with a “shelflife’. The shelflife represents the period for which the Feagentis suitable for use, if stored correctly. This shelf-life shall not be exceeded. Any reagents that are not completely used by the expiry of the shelf-life date shall be discarded. NOTE Often the shelf-life of reagents is supplied by the receiving laboratory. Check reagents periodically, eg by field blanks, and discard any reagent found to be unsuitable. Between on-site visits, reagents shall be stored separately from sample containers and other equipment ina clean, secure cabinet in order to prevent contamination. Each sample shall be labelled accordingly, after the addition of the preservative. Otherwise, there could beno visible indication as to which samples have been preserved, and which have not, 5.1 Solids, 5.11 Sodium thiosulfate pentahydrate, Na2S203-5H20, w(Na2S203-SH20) > 99 %. SAL2 Ascorbic acid, CeHa0s, w(CeHa0¢) > 99 %. 5.1.3 Sodium hydroxide, NaOH, w(NaOH) > 99 %, 5.14 Sodium tetraborate decahydrate, Na2B407-10H20, w(NazB,07-10H20), > 99 %, CAUTION — Sodium tetraborate decahydrate is known to be a carcinogen, mutagen and reproductive toxin (CMR). 5.1.5 Hexamethylenetetramine (hexamine, urotropine), CeH2N¢, w(CoH12N4) > 99 %. 5.1.6 Potassium iodide, KI, w(KI) > 99 %. 5.1.7 Iodine, lo, (lz) > 99 %, 5.1.8 Sodium acetate, CzH3NaQz, w(C2H3Na02) > 99 %. 5.1.9 Ethylenediamine, CzHgNo, w(CzHeN2) > 99 %, 5.2 Solutions, 5.2.1 Zinc acetate solution C4He04Zn (10 g/l). Dissolve 10.0 g of zinc acetate in ~100 ml of water . Dilute to 100 ml with water. Store the solution in a polypropylene or glass bottle for a maximum period of 1 a, 52.2 Orthophosphoric acid (p ~ 1,7 g/ml), H3POs, w(H3P0s) > 85%, c(H3POs) = 15 mol/! 5.2.3 Hydrochloric acid (p ~ 1,2 g/ml), HCl, w(HCl) > 36 %, c(HCI) = 12,0 mol/L 5.24 Nitricacid (p = 1,42 g/ml), HNOs, w(HNOs) > 65 %, c(HNOs) = 15,8 mol/l 5.2.5 Sulfuric acid (p = 1,84 g/ml), H2S0, (freshly prepared). Dilute concentrated sulfuric acid (HS04),p = 1,84 g/ml, w(H2SO4) = 98 % 1 + 1 by carefully adding the concentrated acid to an equal volume of water and mix. (© 150 2018 ~All iGHEHEEEERGhe wo dquo por CONFEDERACION HIDROGRAFICA DEL EBRO et o182: a pode en un sera de redinern, ber digener ela core bee fe AENORISO 5667-3:2018(E) WARNING — Adding the concentrated acid to the water can give violent reactions because of an exothermic reaction, 5.2.6 Sodium hydroxide solution (p = 0,40 g/ml), NaOH. 5.2.7 Formaldehyde solution (formalin), CH20, p(CH20) = 37 % to 40 % (freshly prepared), WARNING — Beware of formaldehyde vapours. Do not store large numbers of samples in small work areas. 5.2.8 Disodium salt of ethylenediaminetetraacetic acid (EDTA) (9 ~ 0,025 g/ml), CioH14N2NazOg:2H20, w(CioHs4NzNa20s:2H20) > 99%, Dissolve 25 g EDTA in 1 000 ml of water. 5.2.9 Ethanol C2HsOH, p(CzHsOH) = 96 %. 5.2.10 Alkaline Lugol's solution, 100 g potassium iodide (5.1.6), 50 g iodine (5.1.7), and 250 g sodium acetate (5.1.8) in 1 000 ml water to pH 10. 5.2.11 Acidic Lugol's solution, 100 g potassium iodide (5.1.6), 50 g iodine (5.1.7) and 100 ml glacial acetic acid (5.2.17) in 1000 ml water to pH 2, 5.2.12 Neutralized formaldehyde solution, formaldehyde solution (5.2.7) neutralized with sodium tetraborate (5.1.4) or hexamethylenetetramine (5.1.5). Formalin solution at 100 g/l gives a final solution of o(CH20) = 3,7 % to 4,0%, WARNING — Beware of formaldehyde vapours. Do not store large numbers of samples in small work areas. 5. Ethanol (5.2.9), formaldehyde solution (5.2.7) and glycerol (5.2.18) (100 + 2 + 1 parts by volume, respectively). 13 Ethanol preservative solution. 5.2.14 Sodium hypochlorite NaOCl, w(NaOCI) = 10 %, Dissolve 100 g sodium hypochlorite (NaOCl) in 1.000 ml of water. 5.2.15 Potassium iodate KI03, w(KI03) = 10 %. Dissolve 100 g potassium iodate (KIO3) in 1 000 ml of water 5.2.16 Methanoic acid (formic acid) CH20z, (CH202) > 98 %. 5.2.17 Glacial acetic acid C2H402, w(C2H402) > 99%. 5.2.18 Glycerol (glycerin, glycerine) C3Hs(OH)3 5.3. Materials. 5.3.1. Container and cap, types as specified in Tables A.1 to A.3, 5.3.2 Filter, pore size 0,40 um to 0,45 um, unless a different filter size is specified in the analytical International Standard. 4 te acumen ha sido aqui por CONFEDERACION HIDROGRAFICA DEL EARUADLANIA,- All rights reserved ara per uaa en un terns deed interno debra pcs dela corepaete beni de ABNOR180 5667-3:2018(E) 6 Containers 6.1 Container selection and preparation ‘The choice of sample container (5.3.1) is of major importance and ISO 5667-1 provides some guidance on this subject. Details of the type of container used for the collection and storage of samples are given in Tables A. ahs cee Same considerations given to this selection of suitable container material shall also be given to the selection of cap liner materials. Sample containers shall be made of a material appropriate for preserving the natural properties of both the sample and the expected range of contaminants. Suitable types of containers for each analyte tobe measured are give toA3. Mere cont Very low concentrations of metals, containers prescribed can be different from those used for higher concentrations. Details can be found in Table A.1 or in the analytical International Standards, [aie Samples are tobe frozen, suitable containers, uch as polyethylene (PE) or polytetrafluoroethylene (PTFE), shall be used to prevent breakage. The use of disposables is preferred. Some manufacturers supply containers with a certificate of cleanliness. If such a certificate of cleanliness is supplied, it is not necessary to clean or rinse the containers before use. 6.2. Filtration on site Filtration on site is required in some cases — Groundwaters shall be filtered on site if dissolved metals need to be analysed, — Waters shall be filtered (5.3.2) on site, if this is required according to Annex A. Unless specified otherwise, a filter pore size 0,40 um to 0.45 um shall be used. immediate filtration on sce then the reason and the time between sampling and pas ashe 6.3 Filling the container The container (5.3.1) shall be fille letely unless prescribed differently in Tables A.1 to A.3 or the analytical International Standard used. Ifthe samples are to be frozen as part of their preservation, fample containers shall not be completely filled. This isin order to prevent breakage which may arise from expansion of ice during the freezing and thawing process. It no preservatives are present in the bottle, then prerinsing the bottle may be advisable. Guidance on prerinsing can be found in ISO 5667-14, 7 Sample handling and preservation Waters, particularly fresh waters, waste waters and groundwaters, are susceptible to changes Se mle oy Rtysical, chemical or biological reactions which may take place between the time of Sfmpling and the commencement of analysis. The nature and rate of these reactions are often such that if precautions are not taken during sampling, transport and storage [for specific analytes), the concentrations determined are different to those existing at the time of sampling, The extent of these changes is dependent on the chemical and biological nature of the sample, its Femperature; its exposure to light, the type of the container in which itis placed, the time between 5 (© 180 2018 - All righsieseevtn side agus por CONFEDERACION HIDROGRAFICA DEL EBRO cl a019-11 aa poder will en un ae dered intro deers pone de a coespoene Hence de AEMORISO 5667-3:2018(E) sampling and analysis, and the conditions to which it is subjected, e4. agitation during transport. Further specific causes of variation are listed in a) to f). a) The presence of bacteria, algae and other organisms can consume certain constituents of the samples. These organisms can also modify the nature of the constituents to produce new constituents. This biological activity affects, for example, the concentrations of dissolved oxygen, carbon dioxide, compounds of nitrogen, phosphorus and, sometimes, silicon. BD) Certain compounds can be oxidized either by dissolved oxygen present in the samples, or by atmospheric oxygen [e.g. organic compounds, Fe(II) and sulfides). “Certain substances can precipitate out of solution, eg. calcium carbonate, metals, and metallic compounds such as Al(OH)3, or can be lost to the vapour phase (e.g. oxygen, cyanides, and mercury) Absorption of carbon dioxide from air can modify pH, conductivity, and the concentration of dissolved carbon dioxide. Passage of compounds like ammonia and silicon fluoride through some types of plastics may also affect pH or conductivity. Dissolved metals or metals in a colloidal state, as well as certain organic compounds, can be irreversibly adsorbed on to the surface of the containers or solid materials in the samples. f) Polymerized products can depolymerize, and conversely, simple compounds can polymerize. Changes to particular constituents vary both in degree and rate, not only as a function of the type of water, but also, for the same water type, as a function of seasonal conditions. These changes are often sufficiently rapid to modify the sample considerably in a short time. In all cases, it is essential to take precautions to minimize these reactions and, in the case of many analytes, to analyse the sample with a minimum of delay. If the required precaution for changes is filtration on site, then a filter (5.3.2) shall be used. Details of the sample preservation are given in Table A.t, 7.2. Sample handling and preservation for biological examination The handling of samples for biological examination is different from that for samples requiring chemical analysis. The addition of chemicals to the sample for biological examination can be used for either fixation and/or preservation of the sample. The term “fixation” is defined as the protection of ‘morphological structures, while the term “preservation” is defined as the protection of organic matter from biochemical or chemical degradation. Preservatives, by definition, are toxic, and the addition of preservatives may lead to the death of living organisms. Prior to death, irritation may cause the most delicate organisms, which do not have strong cell walls, to collapse before fixation is complete. To minimize this effect, itis important that the fixation agent enter the cell quickly. IMPORTANT — Acidic Lugol’s solutions (5.2.11) can lead to the loss of structures in organisms or also lead to the loss of small organisms (e.g. some flagellates); in this case, use an alkaline Lugol's solution (5.2.10), e.g. during the summer, when the appearance of silico-flagellates is frequently observed. ‘The fixing and/or preservation of samples for biological examination shall meet the following criteria a) the effect of the fixative, and/or preservative, on the loss of the organism shall be known beforehand; b) the fixative or preservative shall effectively prevent the biological degradation of organic matter at least during the storage period of the samples; ©) _ the fixative, and/or preservative, shall enable the biological analyte (e.g, organisms or taxonomical groups) to be assessed during the storage period of the samples. Details of the preservation of samples are given in Table A.2. € xe docuento he so add por CONFEDERACION HIDROGRAFICA DEL EARESPLAAB,- All rights reserved ar pode vl en sen ded ter, ter poe de cep ncade ABNORISO $667-3:2018(E) 7.3. Sample handling and preservation for radiochemical analysis. WARNING — Radioprotection such as shielding may be necessary, depending on the activity of the sample. There is little difference between the handling of samples for radiochemical analysis and the handling of samples for physicochemical analysis. The delay between sampling and measurement has to be consistent with the radioactive half-life of the radionuclides of interest. The conditions to be taken for adequate storage are independent of the radioactive half-life, but identical to those required for the corresponding stable isotope. NOTE Cooling radiological samples is primarily used to prevent algal growth and biological spoilage. It is not a necessary preservation step for radiochemical analyses. These samples are often combined with those for physical, chemical or biological analysis. 8 Sample transport Cooling or freezing procedures shall be applied to samples to increase the time period available for transport and storage and if required by Tables A.1 to 4.3. When transport takes place, the sampling plan (e.g. ISO 5667-1) shall conside — the time between sampling and start of transport; — transport time; — starting time of analysis in the laboratory. This sum of these three periods is limited to the maximum storage times according to Tables A.1 to A. If the maximum storage time cannot be met, then the sampling plan shall be reformulated to allow these requirements to be accommodated, A cooling temperature of the device during transport of (5 # 3) °C has been found suitable for many applications. Cooling and freezing procedures applied shall be in line with instructions from the analytical laboratory. Freezing especially requires detailed control of the freezing and thawing process in order to return the sample to its initial equilibrium after thawing. Containers holding samples shall be protected and sealed during transport in such a way that the samples do not deteriorate or lose any part of their content. Container packaging shall protect the containers from possible external contamination, particularly near the opening, and should not itself be a source of contamination Glass containers shall be protected from potential breakage during transport by appropriate packaging. Sepusil be transported as soonas possible after sampling and with cooling necessary according to to Laboratory samples for dispatch or transport by third parties and preserved laboratory samples should be sealed in such manner that the integrity of the sample can be maintained. Samples required for (potential) regulatory investigations should be sealed to a level that meets the requirements of the authorities or other organization(s) concerned with the transport of the sample. tation samples shall be stored in a cooling device capable of maintaining a temperature aluation of the conditions during transport, al cording t rature of the air surrounding the sample NOTE Devices capable of logging of the air temperature during the transportation are available, but their use and adequate calibration can be costly. (© 1S0 2016 - All rightseastnths side adqucde por CONFEDERACION HIDROGRAFICA DEL EBRO dl aai¢2-1 Prayer wlizco ca un nema dere tne, deber apoer de coed iencia de ABNORISO 5667-3:2018(E) 9 Identification of samples Container labels should withstand wetting, drying and freezing without detaching or becoming illegible. The labelling system shall be waterproof to allow use on site. The exact information given in the sampling report and on the sample labels depends on the objectives of the particular measurement programme. In all cases, an indelible label shall be secured to the sample container. For each sample, at least the following information shall be available. — Aunique identifier, traceable to — date, time and location of sampling; — sample number; — description of sample; — name of sampling personnel; — details of sample preservation, or fixation used; — details of sample storage used; — any information regarding integrity and manipulation of the sample; — any other information, as necessary. — Aunique identifier, traceable to sample date, location, and sample number shall appear on the label of the sample container. All other information is supplementary and should be detailed in the sample report. 10 Sample reception All information regarding sample, handling and storage shall be included in a sampling report. Laboratory staff shall receive and check information on sample preservation and sample transport conditions, In all cases, and especially when a “chain of custody” process needs to be established, the number of sample containers received in the laboratory shall be verified against the number of sample containers submitted. 11 Sample storage ‘The storage duration of the water samples within the laboratory is specific to the analyte(s) to be analysed. Samples shall be stored no longer than the maximum storage period given in Tables A.1 to |\5. The maximum storage me of t ratory (1.4) The refrigeration conditions within the laboratory shall be 2) °C, Where samples are frozen for preservation, unless otherwise specified, the temperature shall be maintained below ~18 °C. Exceptions to these refrigeration conditions are listed in Tables A.] to A.3. When thawing frozen samples, it is recommended that each sample container be placed in a separate secondary container to minimize the risk of liquid loss, should a split become apparent during the thawing process or a rupture occur during initial freezing and storage. A mild impact can cause splitting of some plastics at low temperatures. a Ee documento ha so adgudo pur CONFEDERACION HIDROGRAPICA DEL ERASPLZOIB- All rights reserved Para poser utr en un sists dered interna, debe poner del crept liceciade AENORISO 5667-3:2018(E) With respect to thawing, itis recommended that this be done under ambient conditions, unless specified otherwise in Tables A.1 to A.3 or the analytical International Standard being used. (© 150 2018 ~ Allright uREBRENEH. do adqurido poe CONFEDERACION HIDROGRAFICA DEL EBRO 1201021 9 Pac poder iirc un user dered intern, deer poner dea cortepaiet iencia de ABNORISO 5667-3:2018(E) Annex A (informative) Techniques for sample preservation AA General This document and the analytical International Standards listed in this annex are complementaty. See the Notice on page 1. nntradict each other. It is intended that reservation tect cribed in aon mer Cesc nthe aly ieroat used, the technique(s) listed in this document shall be used. A validation protocol used for validation studies can be found in Annex C. Reports and data regarding validation are listed in the bibliography. A.2 Abbreviations for plastics FEP perfluoro(ethylene/propylene) PFA perfluoroalkoxy (polymer) PE polyethylene PP polypropylene PE-HD high density polyethylene PTFE polytetrafluoroethylene PET polyethylene terephthalate PVC poly(vinyl chloride) A3. Physicochemical and chemical analysis See Table A.J. The following general remarks should be noted in relation to use of Table A.1. — Apreservation time of 1 d means that if 24 h is exceeded, this should be stated in the report. — The types of containers are identical to those in the analytical International Standards, In some cases, the type of container in the standard is very specific, e.g. PTFE. This is essential when very low concentrations have to be measured. In other cases, when the specific type of plastic is not important, the term plastics is sufficient. A.4 Biological analysis The following general remarks should be noted in relation to use of Table A.2. — Plastics used for containers in the laboratory are for instance PE, PTFE, PET, PP, PFA, and FEP. — Ifa preservation period is not specified, it is generally unimportant. The indication “1 month" represents preservations without particular difficulty. 10 te documento he sie dur por CONFEDERACION HIDROGRARICA DEL EARIAPARAIB,- All rights reserved ‘Pam poder iil nun sistema dred ier, debe donee dela conepotn lenin de AENORISO 5667-3:2018(E) AS. Radiochemical analytes and activities The following general remarks should be noted in relation to use of Table A.3. WARNING — Radioprotection such as shielding may be necessary, depending on the activity of the sample. — Acidification is carried out to avoid algal growth, biological spoilage, and adsorption of metal ions to the inner wall of the sample container, — Contamination of the sample should be avoided, especially if the sample activity is very low. Some sample sites can have measurable activity in the soil or air, or in waters other than those being sampled. Laboratories, as well as some items of domestic equipment, can contain radioactive material. When sampling precipitation, any special requirements in this table are additional to those given in ISO 5667-8. As the collection of sufficient sample can require a period of days, both the starting and finishing times and dates should be recorded. A record of precipitation collection for the sample station for the appropriate period should be appended. Stabilizer or carrier may be added, if appropriate for the analytes being measured, — Plastics used for containers in the laboratory are for instance PE, PTFE, PET, PP, PFA, and FEP. NOTE _ Some plastics bottles slowly concentrate samples over a period of many months by being very slightly permeable to water. 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