Professional Profile

A proactive, multitasking, determined, detailed oriented and team player chemist

with five years of pharmaceutical/academic experience in analytical chemistry a
nd biotechnology and three years of leadership and management in a Startup Compa
ny. Interested in a research position in which the problem solving skills and w
ork experience can enhance my knowledge personally and professionally and can be
applied in solving challenging cross-functional problems.
Summary of Qualification
* Experienced in analytical instrumentation including High Throughput library an
alysis by HPLC-MS-ELSD, SFC-MS-ELSD; multiplexed CE, HPCE, Prep-LC, GPC, fast GC
, GC-MS, ICP-MS, UV-Vis spectroscopy, potentiometers (pH, conductivity), CCD, PD
A, and Laser wave mixing spectroscopy
* HPLC and fast GC method development for the analysis, purification and separat
ion of active pharmaceutical ingredient (API)
* Protein separation and detection at lowest LOD (yoctomole) by capillary electr
ophoresis (CE) and laser wave-mixing spectroscopy,
o Using synthesized amphiphilic polymers
o High throughput CE
o In native form at UV range
* Single cell imaging and protein analysis by laser wave-mixing spectroscopy
* Knowledge of cell culture (Jurkat and Hela cancer cells) for single cell prote
in analysis purposes
* Synthesis and characterization of linear amphiphillic polymers using linear so
lvation energy relationship (LSER) and associated solvation properties
* Familiarity with TOF-MS
* Familiarity with FTIR (Fourier Transform Infrared Spectroscopy)
* Proficient with MS Word, Excel, PowerPoint
* Ability to work both independently and on cross-functional project team

Work Experience
Scientist 2004- 2008
Pfizer Inc. La Jolla, CA
* Performed High Throughput Pre and Post Prep library analysis by LC-MS-ELSD and
SFC-MS-ELSD at early stage of drug discovery
* Developed methods for API analysis using HPLC, Prep-LC, LC-MS for different pr
ojects in Analytical and Chemical R & D departments (ARD and CRD)
* Developed and created fast GC method for simultaneously analysis of 27 solvent
s currently used by the company, installed the method on the CRDS website as a g
eneral method for further use by analysts
* Provided Fast GC training to ARD and CRD analysts
* Supported CRD and World Wide Safety Sciences (WWSS) projects by performing dru
g substance and intermediate sample preparation and analysis of specific element
s/Heavy metal by ICP-MS
* Supported method development for chiral separation of API using HPLC
* Evaluated all the stored and used HPLC column in Chemical R & D Support (CRDS)
group for future use
* Maintained and troubleshoot HPLCs, installed all the HPLC STMs on company webs
ite and on the requested HPLCs throughout the ARD and CRD departments
* Trained and supervised undergraduate students on wet chemistry, HPLC, GC and I
CP-MS
Lecturer 2002- 2003
San Diego State University San Diego, CA
* Instructed General Chemistry II laboratory
Chemistry Lab instructor 1999- 2002
North Carolina State University Raleigh, NC
* Instructed two courses of Analytical Chemistry laboratory
* Instructed six classes of General Chemistry laboratory
* Teaching Assistant in problem sessions and grading General Chemistry I
Technical Director-Co-founder 1995-1997
PDT Co. LTD. Tehran, Iran
(Water and Wastewater Analysis Systems)
* Advised EPAs, Universities' Health departments, Tehran Oil refinery, National
Gas Co., Water and wastewater companies throughout the country for choosing the
right water and wastewater equipments based on their needs
* Installed, set up and instructed the end users on water and wastewater analysi
s instruments
* Responsible for holding International Exhibition on Water and Wastewater Analy
sis systems
* Received comprehensive training of water and wastewater systems in UK
Customer Service Manager 1994-1995
Kherad Kia Co. Tehran, Iran
(Water and Wastewater Analysis Systems)
* Provided customer services including replacing, repairing and ordering parts a
nd chemicals
* Responsible for inventory ordering and parts replacement
Technical Sales Associate 1993-1994
* Held technical seminars to introduce and advertize water and wastewater analys
is systems
* Translated Brochures and Manuals for local customers
* Provided instruction and tutorial to end-users
Education
San Diego State University/ San Diego, CA 2002-2008
North Carolina State University, Raleigh NC 1999- 2008
PhD in Analytical Chemistry with a minor in Biotechnology
Advisors:
Dr. William Tong, Distinguished Professor, SDSU
Dr. Morteza Khaledi, Chair of chemistry department, NCSU
Dissertation title: "Protein Analysis at the Single Cell Level by Nonlinear Lase
r Wave-Mixing Spectroscopy for High Throughput Capillary Electrophoresis Applica
tions"
University of Tehran, Tehran, Iran, BS in Applied Chemistry 1988-1993
Azad University, Tehran, Iran, BA in English Language, Translation work 1
987-1992
Selected Academic Projects
* Ultra sensitive detection (yoctomole level) of native protein standards and ce
llular proteins by CE and UV laser wave-mixing spectroscopy
o Achieved shorter analysis time due to non-labeling procedure
* High throughput protein separation by CE and laser wave-mixing spectroscopy
o Collected wave mixing signals of labeled proteins in a capillary array of 10 s
quare capillaries using photo diode array detector
o Obtained the lowest detection limit (yoctomole) for proteins
* Polymer-based protein separation by CE and laser wave-mixing spectroscopy
o Synthesized amphiphilic polymer
o Investigated its application in protein separation
o Compared the results with common sieving matrix
* Single cell on-column protein analysis and detection using laser wave-mixing s
pectroscopy and SDS-Dalt-CE in UV-Visible range
o Coated the capillary
o Developed a new injection technique for single cell injection into the capilla
ry
o Performed on-column cell lysis and protein labeling
* Single cell imaging by laser wave-mixing spectroscopy
o Cultured Jerkat cells
o Labeled its mitochondria
o Used new technique for docking single cell in micro channel and CE chip
o Obtained 2D images and mapped the mitochondria by laser wave mixing spectrosco
py and CCD camera
* Synthesis of reversible gel like sugar based polymers
o Investigated their application in chromatography and drug delivery
o Investigated their retention factor for different analytes in basic and acidi
c pH by using pH sensitive cross linker
* Synthesis and characterization of linear amphiphilic polymeric micelles by cap
illary electrophoresis
o Purified the synthesized polymers (dialysis and GPC)
o Assessed solubility, pH, concentration and temperature variation of the synthe
sized polymers
o Determined its solvation properties through Linear Solvation Energy Relationsh
ip (LSER)

Manuscripts (In review for publication)

Behrokh B. Sadri and William G. Tong, Native Cellular Proteins Separation by Cap
illary Electrophoresis and Non-linear Laser Wave-Mixing Spectroscopy
Behrokh B. Sadri and William G. Tong, High-Throughput Protein Separation by Capi
llary Electrophoresis with Non-linear Laser Wave-Mixing Spectroscopy detection
Behrokh B. Sadri, William G. Tong, Amphiphilic Polymer based Protein Separation
by Capillary Electrophoresis and Nonlinear Laser Wave mixing Spectroscopy
Behrokh B. Sadri, Adrian A. Atherton and William G. Tong, Single-Cell Protein A
nalysis by Capillary Sieving Electrophoresis (CSE) and Laser Wave-Mixing Spectro
scopy
Adrian A. Atherton, Behrokh B. Sadri and William G. Tong, Imaging of Distributed
Absorbing Species within a Single Cell by Nonlinear Laser Wave Mixing Spectrosc
opy

Conference Presentation
41st ACS Western Regional Meeting, San Diego, CA 2007
Poster Presentation on High-Throughput Protein Analysis by Capillary Electrophor
esis and Sensitive Laser Wave-Mixing Detection
Poster Presentation on Multi-Photon Laser Wave-Mixing Analysis of Cellular Prote
ins
40th ACS Western Regional Meeting, Anaheim, CA 2006
Poster Presentation on Polymer-based capillary electrophoresis with laser wave-m
ixing detection
229th ACS National Meeting, San Diego, CA, 2005
Poster presentation on Single cell protein analysis and separation using non-lin
ear laser Wave-mixing spectroscopy
17th Annual CSU Biotechnology Symposium, Los Angeles, CA 2005
Poster presentation on Single cell detection and analysis using non-linear laser
wave mixing spectroscopy
Pittcon Conference, Chicago, IL 2004
Oral presentation on Tailoring the structure of amphiphilic polymer using Capill
ary Electrophoresis
Professional Affiliation
* American Chemical Society (ACS)

Awards
* Recognition award (Pfizer Inc.): 2005
o Developing and implementing Fast GC method and training analysts in its use
o Independent work using ICP-MS for Pfizer Worldwide Safety Sciences (WWSS) samp
les
References
1) Dr. William Tong
San Diego State University,Professor
Phone(619)594-2442
E-mail: william.tong@sdsu
.edu
2) Dr. Morteza G. Khaledi
North Carolina State University, Professor
Phone(919)-515-2943
E-mai
l: morteza_khaledi@ncsu.edu
3) William Farrell
Pfizer Inc, Discovery, Chemistry Department, Manager
Phone: (858) 638-3658, (858) 354-6463
E-mail: william.farrell@pfizer.com