Академический Документы
Профессиональный Документы
Культура Документы
SOSA
2419 Mission Street a San Francisco, California 94110
310-980-2689 a ss107b292@westpost.net
PROFESSIONAL EXPERIENCE
2007-2010: Sabbatical due to injury recovery.
VAXGEN, South San Francisco, California a 2004-2007
Manufacturing Associate II: Assisted in developing and manufacturing vaccines to
defend against bioterrorism attacks. Prepared manufacturing process solutions.
Managed Tangential Flow Filtration (TFF) on Millipore workstation. Maintained GM
P cleanroom facility; executed, revised, and drafted SOPas. Prepared buffers and
conducted GMP inspections of raw materials and printed forms. Assisted in devia
tion investigations and CAPA initiatives. Updated training qualifications. Perfo
rmed protein purifications using Ion Exchange Chromatography (IEX), Hydrophobic
Interaction Chromatography (HIC), and Size Exclusion Chromatography (SEC). Overs
aw formulation, filtration, and aliquot of vaccine. Ensured proper Clean in Plac
e procedures for instrumentation.
a Assured proper storage of purification columns by designing and implementing s
afe storage procedures.
a Secured drastic improvement in solutions prep facility safety record by reduci
ng contact with caustics.
a Chosen to administer associate training in GMP manufacturing procedures.
FIBROGEN, South San Francisco, California a 1997-2003
Research Associate II: Assisted in producing proteins used in developing biologi
cal materials for tissue and scar repair. Oversaw and maintained mammalian, bacu
lovirus insect cell and E.coli protein-producing expression systems. Utilized HP
LC to separate and characterize organic compounds and proteins. Isolated proteas
e inhibitors through HTS library screening; created assays to screen small molec
ule inhibitors against multiple proteases. Performed gel filtration, IEX, HIC, a
nd antibody affinity chromatography. Maintained lab inventory; oversaw materials
including liquid nitrogen, dry ice and gas supplies. Streamlined communications
between sales team and end users. Set up vendor shows. Established and validate
d HPLC and FPLC methods.
a Increased protein purification and yield on Pharmacia and BioCad FPLC workstat
ions.
a Boosted CTGF yield by 90% by modifying procedure to make product less adherent
to surfaces.
a Stockpiled proteins including BMP-1, CTGF, N-proteinase, TGF2, and mAb 19 ahea
d of deadline.
a Adjusted E.coli inclusion body isolation to heighten production of dimerized,
soluble TGF2.
a Leveraged HTS information in deducing and synthesizing small molecule inhibito
rs to BMP-1.
EDUCATION
Bachelor of Science in Biochemistry
San Francisco State University, San Francisco, California
TECHNICAL SKILLS
MS Office a Visio a Adobe Photoshop a Adobe Illustrator