Charm® Lateral Flow Mycotoxin Tests at a Glance

Charm ROSA® (Rapid One Step Assay) Quantitative Tests

Extraction Incubation Time USDA GIPSA† Charm-Approved‡ Reader Range
Approved Commodities Commodities
H
H

EO OH
EO
EO

Aflatoxin Quantitative Test

E
M

M
M

H
EO
H
EO OH

M
E

M
M

H
EO
H

AFQ-004
OH

H
EO
M
M

M
OH
H

70% Methanol 10 Min Corn Flaking Corn Grits Barley 0 - 150 ppb
Corn Flour Milled Rice Basmati Rice
Corn Germ Meal Oats Brewer’s Rice
Corn Gluten Meal Popcorn Millet
Corn Meal Rough Rice Wheat Flour
Corn Screenings Rye
Corn/Soy Blend Sorghum
Cracked Corn Soybeans
DDG Wheat
DDGS
84% Acetonitrile 10 Min N/A Corn Gluten Feed 0 - 150 ppb
Cottonseed Hulls
Cottonseed Meal
Peanut Hulls
Whole Cottonseed
E
O
ET
OH

OH

Aflatoxin Quantitative Test - Ethanol Extraction

ET

OH

ET

OH
ET
O

OH

ET
OH

ET

OH
ET

OH

ET

OH

AFQ-ETOH-004
H

ET

OH

ET
OH

ET

OH

50% Ethanol 10 Min N/A Barley Oats 0 - 150 ppb

Brewer’s Rice Peanut Hulls
Corn Rice Bran
Corn Bran Rice Hulls
Corn Germ Meal Sorghum
Corn Gluten Meal Soybean Meal
Cottonseed Hulls Wheat
Cottonseed Meal Wheat Bran
DDGS Wheat Midds
Milled Rice
TS
T
S

FA
T
FA
S

Fast Aflatoxin Quantitative Test

FA

T
S

S
ST
FA

FA
O

FA

T
S

S
T
S

FA

FA
FA

AFQ-FAST-004
F
T

T
S

S
T

T
FA

S
FA

FA
T

70% Methanol 3 Min Corn N/A 0 - 150 ppb

DONQ-004
DON Quantitative Test

Deionized or 10 Min Barley Rough Rice Brewer’s Rice 0 - 6 ppm

Distilled Water Corn Sorghum Corn Bran 6 -12 ppm
Malted Barley Wheat Corn Germ Meal 12 - 24 ppm
Milled Rice Wheat Flour Corn Gluten Meal
Oats Wheat Midds DDGS
Rice Bran
Soybean Meal
M
H
H

H
EO
EO

EO

Fumonisin Quantitative Test

M
M

H
EO
H

H
EO

EO

M
M

H
EO
H

FUMQ-004
H

H
EO

EO

EO
M
M

M
OH
H

70% Methanol 10 Min Corn DDGS 0 - 6 ppm

Sorghum 6 - 60 ppm
E
O
ET
OH

OH

Fumonisin Quantitative Test - Ethanol Extraction

ET

OH

ET

OH
ET
O

OH

ET
OH

ET

OH
ET

OH

ET

OH

FUMQ-ETOH-004
H

ET

OH

ET
OH

ET

OH

50% Ethanol 10 Min N/A Barley 0 - 1 ppm

Corn 0 - 6 ppm
DDGS 6 - 60 ppm
Soybean Meal

†
United States Department of Agriculture, Grain Inspection Packards & Stockyards Administration
‡
In-house validation. All GIPSA-appoved commodities are also Charm-approved commodities.

Charm Sciences, Inc. 659 Andover Street, Lawrence, MA 01843, USA | Tel: +1.978.687.9200 | Fax: +1.978.687.9216 | Email: info@charm.com | www.charm.com
© Copyright 2010 Charm Sciences, Inc.
 Charm ROSA® (Rapid One Step Assay) Quantitative Tests (continued)
Extraction Incubation Time USDA GIPSA† Charm-Approved‡ Reader Range
Approved Commodities Commodities

Ochratoxin Quantitative Test

OCHRA-003

70% Methanol 10 Min Barley Corn Gluten Meal 0 - 12 ppb

Malted Barley 10 - 150 ppb
Wheat

T2/HT2-001
T2/HT2 Quantitative Test

70% Methanol 10 Min N/A Barley 0 - 250 ppb

Corn 0 - 2500 ppb
Corn Gluten Meal
Oats
Wheat
H
H

Zearalenone Quantitative Test

EO
EO

EO
M
M

H
EO
H

H
EO

EO

M
M

H
EO
H

ZEARQ-004
H

H
EO

EO

EO
M
M

M
OH
H

70% Methanol 10 Min Barley Oats N/A 0 - 1400 ppb

Corn Rough Rice
DDGS Sorghum
Flaking Corn Grits Wheat
Milled Rice Wheat Flour
ET
O
ET
OH

Zearalenone Quantitative Test - Ethanol Extraction

OH
ET

OH

O
ET

ET
ET
O

OH

OH
OH

ET

ET
ET

ZEARQ-ETOH-004
H

OH
H

O
ET

ET

ET

50% Ethanol 10 Min N/A Corn 0 - 1400 ppb

Corn Meal
Wheat

Charm ROSA® (Rapid One Step Assay) Positive/Negative Qualitative Tests

Extraction Incubation Visual USDA GIPSA† Charm-Approved‡ Threshold
Time Option Approved Commodities Commodities

Aflatoxin P/N Test

50% Ethanol 3 Min NO N/A Corn 10 ppb

70% Methanol 3 Min NO Corn N/A 10 ppb
50% Ethanol 3 Min YES Corn N/A 20 ppb
70% Methanol 3 Min YES Corn N/A 20 ppb
BEST BEST BES
BEST BEST BEST

BEST BEST BEST

T BEST BEST B
EST BEST BEST

ST BEST BEST

AFPN-BEST-004
BEST Aflatoxin P/N Test

Biodegradable 3 Min NO Corn – Submitted Corn 10 ppb

Extraction
Solution 3 Min YES Corn – Submitted Corn 20 ppb

DONPN-005
DON P/N Test

Deionized or 3 Min YES Wheat N/A 0.5 ppm

Distilled Water 3 Min YES Barley Corn 1 ppm
Wheat
3 Min YES N/A Corn, Wheat 2 ppm

3 Min YES N/A Corn, Wheat 5 ppm

†
United States Department of Agriculture, Grain Inspection Packards & Stockyards Administration
‡
In-house validation. All GIPSA-appoved commodities are also Charm-approved commodities.

Charm Sciences regularly qualifies new commodities to best fit our customer’s needs.
Please contact Charm Sciences for the most updated list of approved commodities.

Charm Sciences, Inc.

659 Andover Street, Lawrence, MA 01843-1032, USA
Tel: +1.978.687.9200 | Fax: +1.978.687.9216 | Email: info@charm.com | www.charm.com
© Copyright 2010 Charm Sciences, Inc. Charm and ROSA are registered trademarks of Charm Sciences, Inc. MRK-102-002 0310