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  • Featured Product of Selleck - PF-2341066l (Purity 99%)

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    Selleck Chem
    19 просмотров1 страница
    Technical Data: M.Wt: 450.34 Formula: C21H22Cl2FN5O Solubility: DMSO Purity: >99% Storage: at -20℃ 2 years CAS No.: 877399-52-5 Biological Activity of PF-2341066(Crizotinib): Inhibitor of the catalytic activity of c-Met kinase and the NPM-ALK(nucleophosmin- anaplastic lymphoma kinase) fusion protein.[1] A t(2;5) chromosomal translocation resulting in expression of an oncogenic kinase fusion protein known as NPM-ALK has been implicated in the pathogenesis of anaplastic large-cell lymphoma (ALCL). [2] After incubation with PF-2341066, cells were washed once with HBSS supplemented with 1 mmol/L Na3VO4 and protein lysates were generated. Subsequently, phosphorylation of ALK was assessed by a sandwich ELISA method using an immobilized anti–total-ALK antibody and an anti–phospho-ALK antibody (pY1604) as a detec ion antibody. PF-2341066 inhibited NPM-ALKphosphorylation in Karpas299 or SU-DHL-1 ALCL cells (mean IC50 value=24 nM). PF-2341066 potently inhibited cell proliferation, which was associated with G1-S–phase cell cycle arrest and induction of apoptosis in ALK-positive ALCL cells (IC50 values=30 nM) but not ALK-negative lymphoma cells. The induction of apoptosis was confirmed using terminal deoxyribonucleotide transferase–mediated nick-end labeling and Annexin V staining (IC50 values=25–50 nM). [3] References on PF-2341066(Crizotinib): [1] Deininger M et al.Blood 2005,105:2640–53 [2] Duyster J et al.Oncogene 2001,20:5623–37 [3] James G. Christensen1 et al. Mol Cancer Ther December. 2007,6:3314

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    Featured Product of Selleck---PF-2341066l(Purity>99%)

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    Technical Data: M.Wt: 450.34 Formula: C21H22Cl2FN5O Solubility: DMSO Purity: >99% Storage: at -20℃ 2 years CAS No.: 877399-52-5 Biological Activity of PF-2341066(Crizotinib): Inhibitor of the catalytic activity of c-Met kinase and the NPM-ALK(nucleophosmin- anaplastic lymphoma kinase) fusion protein.[1] A t(2;5) chromosomal translocation resulting in expression of an oncogenic kinase fusion protein known as NPM-ALK has been implicated in the pathogenesis of anaplastic large-cell lymphoma (ALCL). [2] After incubation with PF-2341066, cells were washed once with HBSS supplemented with 1 mmol/L Na3VO4 and protein lysates were generated. Subsequently, phosphorylation of ALK was assessed by a sandwich ELISA method using an immobilized anti–total-ALK antibody and an anti–phospho-ALK antibody (pY1604) as a detec ion antibody. PF-2341066 inhibited NPM-ALKphosphorylation in Karpas299 or SU-DHL-1 ALCL cells (mean IC50 value=24 nM). PF-2341066 potently inhibited cell proliferation, which was associated with G1-S–phase cell cycle arrest and induction of apoptosis in ALK-positive ALCL cells (IC50 values=30 nM) but not ALK-negative lymphoma cells. The induction of apoptosis was confirmed using terminal deoxyribonucleotide transferase–mediated nick-end labeling and Annexin V staining (IC50 values=25–50 nM). [3] References on PF-2341066(Crizotinib): [1] Deininger M et al.Blood 2005,105:2640–53 [2] Duyster J et al.Oncogene 2001,20:5623–37 [3] James G. Christensen1 et al. Mol Cancer Ther December. 2007,6:3314

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    19 просмотров1 страница

    Featured Product of Selleck - PF-2341066l (Purity 99%)

    Загружено:

    Selleck Chem
    Technical Data: M.Wt: 450.34 Formula: C21H22Cl2FN5O Solubility: DMSO Purity: >99% Storage: at -20℃ 2 years CAS No.: 877399-52-5 Biological Activity of PF-2341066(Crizotinib): Inhibitor of the catalytic activity of c-Met kinase and the NPM-ALK(nucleophosmin- anaplastic lymphoma kinase) fusion protein.[1] A t(2;5) chromosomal translocation resulting in expression of an oncogenic kinase fusion protein known as NPM-ALK has been implicated in the pathogenesis of anaplastic large-cell lymphoma (ALCL). [2] After incubation with PF-2341066, cells were washed once with HBSS supplemented with 1 mmol/L Na3VO4 and protein lysates were generated. Subsequently, phosphorylation of ALK was assessed by a sandwich ELISA method using an immobilized anti–total-ALK antibody and an anti–phospho-ALK antibody (pY1604) as a detec ion antibody. PF-2341066 inhibited NPM-ALKphosphorylation in Karpas299 or SU-DHL-1 ALCL cells (mean IC50 value=24 nM). PF-2341066 potently inhibited cell proliferation, which was associated with G1-S–phase cell cycle arrest and induction of apoptosis in ALK-positive ALCL cells (IC50 values=30 nM) but not ALK-negative lymphoma cells. The induction of apoptosis was confirmed using terminal deoxyribonucleotide transferase–mediated nick-end labeling and Annexin V staining (IC50 values=25–50 nM). [3] References on PF-2341066(Crizotinib): [1] Deininger M et al.Blood 2005,105:2640–53 [2] Duyster J et al.Oncogene 2001,20:5623–37 [3] James G. Christensen1 et al. Mol Cancer Ther December. 2007,6:3314

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    Product Information

    S1068 PF-2341066
    c-Met

    Novel Life Science Reagents


    for Cell Signaling Research

    Toll Free:
    (877)796-6397
    -- USA and Canada only --

    Fax:
    +1-713-796-9816
    HN

    Technical Data:
    M.Wt: Formula: Solubility: Purity: Storage: CAS No.: 450.34 C21H22Cl2FN5O DMSO 99% at -20 2 years 877399-52-5
    N N N NH2
    (R)

    Orders
    +1-713-922-4115 enquiry@selleckchem.com

    O Cl F

    Tech Support
    tech@selleckchem.com

    Cl

    Synonyms: PF-02341066,

    Crizotinib

    (chemistry support)

    techbio@selleckchem.com
    (bio support)

    Biological Activity
    PF-2341066 inhibited NPM-ALKphosphorylation in Karpas299 or SU-DHL-1 ALCL cells (mean IC50 value=24 nM). PF-2341066 potently inhibited cell proliferation, which was associated with G1-Sphase cell cycle arrest and induction of apoptosis in ALK-positive ALCL cells (IC50 values=30 nM) but not ALK-negative lymphoma cells. The induction of apoptosis was confirmed using terminal deoxyribonucleotide transferase-mediated nick-end labeling and Annexin V staining (IC50 values=2550 nM). [3]

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    References
    [1] Deininger M et al.Blood 2005,105:264053 [2] Duyster J et al.Oncogene 2001,20:562337 [3] James G. Christensen1 et al. Mol Cancer Ther December. 2007,6:3314

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    Goods ordered in error can only be accepted for return if made within 3 days of delivery, are unopened,have been stored correctly and are in good condition. Should you wish to return any item(s), prior authorization must

    Notes
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