Biology Practical

These are a few answers to things that MIGHT come up, obviously I dont know what is going to come up, but revise chapter 1 and topic 4.4 ( that includes anaerobic respiration) in the biology book, also try and do a little research of your own on yeast! 4. Why was the yeast diluted? It separates large clumps of yeast so we can obtain countable results and allows easier comparisons. 5. Why was cotton placed on top of the flask? Prevents contamination and allows airflow. 6. Why did we use sterile conditions? So results not affected by other factors such as bacteria (contamination). 7. Why was the yeast spread across agar plate? So the yeast would be evenly distributed, allowing growth of the yeast cells on the growth medium. 8. Why did we swirl the flask? To distribute the yeast in solution, to prevent settling of the yeast. 9. How can we make the results more reliable (other than repeat the test)? Remove any confounding variables. 10.How did you obtain a random sample? Say that you used a calculator to generate coordinates and expand if you want 11.Limiting factors: oxygen, growth medium ( the growth medium in the agar plate contains nutrients to help the yeast to grow) THESE ARE THE THINGS THAT YEAST ARE COMPETING FOR! 12.Why were you asked to put the yeast in the agar plate immediately/ why were you asked not to fully remove the lid? Talk about contamination and how this could lead to other things such as bacteria growing in the agar plate which you would then mistake for a yeast colony and therefore it would affect your results. 13.How can you increase reliability?

Wear face mask and gloves to avoid human contamination. 14.How would you increase accuracy? 1. Use a microscope. 2. Use a grid with smaller squares because the yeast are small species, this helps obtain more accurate results and so its more representative. 15.How can we increase validity? We only used two oxygen concentrations (large flask and small flask), no intermediates were tested, so we could test yeast in other oxygen concentrations and see if they grew better. 16.The controls of the experiment were? 17.Same start population of yeast cells in both flasks. 18.Same amount of distilled water. 19.Same sized agar plate used, so yeast had the same amount of growth medium and hence the same amount of nutrients. 20.Constant temp. Our independent variable (the thing we changed) was Oxygen. Our Dependant variable (the thing we kept the same) was yeast. YEAST GROWS BETTER IN LOW CONC. OF OXYGEN, AS THEY RESPIRE ANAEROBICALLY! ALSO BE FAMILIAR WITH THIS BACTERIA/FUNGI GROWTH CURVE, WE MAY NEED IT: