Infection by the Influenza Virus Plot Draft 1 Titles etc. Fade from white.

Act 1 Narration notes: Introducing the virus, its method of infection and the main components. Scene 1 The cam pans zooms into a globule of mucus/ saliva that is travelling through the air. (subdued blue and green background) Holding the droplet in frame for a short while before breaking the surface and entering the fluid. Particles float past the lens at varying depths. Some of these particles are black the camera zooms past them and holds one the viruses in shot tracking around it. The background colours change as the droplet is inhaled becoming shades of orange and red. Fade out Scene 2 Rear tracking shot of globule travelling up the nasal cavity past hairs etc. travels with speed as the air is drawn in. The background and environmental colours become ruddier and redder as the camera passes deeper into the body (indicative of heavier blood concentration) Bypassing this preliminary defence the droplet makes its way towards the back of the throat. Camera ceases to track the droplet holding it in frame as it disappears into the gloom. Fade out. Scene3 Fade in to back of throat. Environmental colours are soft fleshy pastel shades occasionally crossed with veins and capillaries below the wet glistening surface. The camera starts to zoom in on the surface of the trachea, this starts to bring details into view. The camera pauses to establish the next shot. The wall of the throat is coated in a fluid below which thousands of tiny cilia pulse in a predetermined rhythm. Faintly below s layer we can see vague shapes of blood vessels. Whilst the cam takes in this view the droplet passes into shot. The camera tracks the globule as it lands in the mucus layer, ripples emanate from the crash

point. Cut to new camera showing several virus being carried away from the crash site by the current created by the cilia which pulse below them. The camera follows one of these virus as it sinks and nestles down into the base of the cilia. (Think anemone).Fade out. Act 2 Narration notes: this act describes the infiltration of the virus into a host cell and then the subsequent RNA replication process. This act takes place within the cell camera movements need to resolved once scale is calculated below is the process before appropriate editing and cinematography. Much of these issues will be resolved during storyboarding. Scene 1 The virus attaches itself to the sialic acid sugars on the surface of the cell with its hemagglutinin. Once attached a protease cleaves the hemagglutinin and the virus is absorbed into the cell by endocytosis. During the process of endocytosis a vacuole forms around the virus. The acidic nature of the endosome causes the hem agglutinin to bond with the wall of the vacuole and the Ion channel(s) draw in protons. These protons will appear as glowing particles floating in the solution, they will be drawn in much like a vacuum cleaner as the numbers increase the ion channel(s) will develop radiating channels that will perforate the virus. (The visual cues are derived from objects powering up, ref: Independence day alien spaceship firing. Reaching critical mass etc.) (Possibly a bit of camera shake) Scene 2 The virus peels apart around the perforations releasing RNA, various proteins and RNA-dependant RNA polymerase into the cytoplasm. These proteins and vRNA combine to form a complex which then travels into the cell nucleus. Here the RNA-dependent RNA polymerase begins producing complementary positive-sense vRNA. (Transcription). Some of the vRNA returns to the cytoplasm and is translated whilst some remains in the nucleus.

Scene 3 Newly synthesised viral proteins are either secreted through the Golgi apparatus onto the cell surface (in the case of neuraminidase and hemagglutinin) or transported back into the nucleus to bind vRNA and form new viral genome particles. Other viral proteins have multiple actions in the host cell, including degrading cellular mRNA and using the released nucleotides for vRNA synthesis and also inhibiting translation of host-cell mRNAs. Negative-sense vRNAs that form the genomes of future viruses, RNA-dependent RNA polymerase, and other viral proteins assemble into a virus. Hemagglutinin and neuraminidase cluster into a bulge in the cell membrane. The vRNA and viral core proteins leave the nucleus and enter this nodule.

Act 3 Narration notes: This act depicts the newly formed viruses emerging from the once healthy host cell, the colour of the cell has turned grey and is starting to darken. The final scene is one of the healthy cells dying off in large dark patches which will eventually fade to black. Scene 1 The mature virus buds off from the cell in a sphere of host phospholipid membrane, acquiring hemagglutinin and neuraminidase with this membrane coat. The viruses adhere to the cell through hemagglutinin, the mature viruses detach once their neuraminidase has cleaved sialic acid residues from the host cell. Scene 2 After the virus has formed the bud the camera pans away from the host cell as it does so the newly emerged virus follows it and lands between the cilia of an adjacent cell. As the camera pans away we see more viruses fruiting and being released into the fluid that coats the trachea. Scene 3

The camera zooms out away from surface of the trachea as patches of dark greyish brown dead cells spread outward from points of infection. Coverage fills the screen which fades to black. Final titles.