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NATIONAL INSTITUTE FOR BIOTECHNOLOGY AND GENETIC ENGINEERING FAISALABAD Subject:ADVERTISEMENT OF TENDER NOTICE #.

22 TO 38/2010

Four copies of Tender Notice # 22 to 38/2010 are enclosed with the request to get the same published in National Newspapers for its circulation throughout the country as specified below:Sr.# 01 02 Name of Newspaper The daily DAWN Karachi & Lahore. The daily Express Lahore. Date of publication 18-08-2010 18-08-2010 Language English Urdu

It is also requested that one copy of each news paper bearing said advertisement may kindly be provided to the undersigned on top priority basis for record and reference for subsequent payment please.

( Muhammad Hussain ) Sr. Admin. Officer (Procurement) Encl: a.a. Admin Officer, NIBGE, Faisalabad. U. O. Note No. 3(275-291)/2010/NIBGE
C.C:1 2 Dr. Aftab Bashir, PS, Incharge IT Centre NIBGE with the request to up-load the said PDF file of the advertisement on NIBGE website. Soft copy has also been provided. Mr. Jamil Ahmed Rathore, Deputy Director, Government of Pakistan Public Procurement Regulatory Authority Cabinet Division, Islamabad, with the request to up-load the said advertisement on PPRA, Web site and send a bill to the undersigned for payment with proper reference of this Institutes Tender Number/date etc please.

Dated: 12-08-2010

3. Dr. Nasir A. Saeed, P.S. Indenter for information please. 4. PPRA,s Email No. PPRA,Islamabad info@ppra.org.pk.

TENDER NOTICE # 22 TO 38/2010

National Institute for Biotechnology and Genetic Engineering (NIBGE), invites sealed tenders from Sales Tax Registered firms for the purchase of Chemicals, Kits, Glasswares, Plastic Wares Labwares and Lab Equipments etc on FOR NIBGE, Faisalabad.
Tender # Item Tender Fee Mode of Purchase FOR NIBGE Date & Time of Tender Receipt 03-09-2010 at 10 .00AM 06-09-2010 at 10 .00AM 07-09-2010 at 10 .00AM 08-09-2010 at 10 .00AM 14-09-2010 at 10 .00AM 15-09-2010 at 10.00 A.M. 16-09-2010 at 10.00 A.M. 17-09-2010 at 10.00 A.M. 20-09-2010 at 10.00 A.M. 21-09-2010 at 10.00 A.M. 22-09-2010 at 10.00 A. M. 23-09-2010 at 10.00 A.M. 24-09-2010 at 10.00 A.M. 27-09-2010 at 10.00 A.M. 28-09-2010 at 10.00 A.M. 29-09-2010 at 10.00 A.M. Date & Time of Tender Opening 03-09-2010 at 1200 hours 06-09-2010 at 1200 hours 07-09-2010 at 1200 hours 08-09-2010 at 1200 hours 14-09-2010 at 1430 P.M. 15-09-2010 at 1430 P.M. 16-09-2010 at 1430 P.M. 17-09-2010 at 1430 P.M. 20-09-2010 at 1430 P.M. 21-09-2010 at 1430 P.M. 22-09-2010 at 1430 P.M. 23-09-2010 at 1430 P.M. 24-09-2010 at 1430 P.M. 27-09-2010 at 1430 P.M. 28-09-2010 at 1430 P.M. 29-09-2010 at 1430 P.M.

22/2010 23/2010 24/2010 25/2010 26/2010 27/2010 28/2010 29/2010 30/2010 31/2010 32/2010 33/2010 34/2010 35/2010 36/2010 37/2010

Chemicals
Consumable/Kits

Rs. 500/-

Rs. 500/Rs. 500/Rs. 500/Rs. 500/Rs:500/Rs:500/Rs:500/Rs:500/Rs:500/Rs:500/Rs:500 Rs:500/Rs:500/Rs:500/Rs:500/-

-do-do-do-do-do-do-do-do-do-do-do-do-do-do-do-

Chemicals/Kits
Glasswares/Plastic wares etc Consumable Plasticwares etc Pipettmen Ultra Low Tempreture Freezer -80 C PCR Machine Laminar Flpw Cabnit Computers and accessories Pipettman Sets Lab Equipments Distillation Still Water Computer and Accessories Electrical Conductivity Meter

38/2010

Freezer -20 C

Rs:500/-

-do-

30-09-2010 at 10.00 A.M.

30-09-2010 at 1430 P.M.

Tender documents can be obtained on request from Sr. Administrative Officer (Procurement) on any working day (except Saturday & Sunday) through only CDR (Nonrefundable) in favour of Accounts Officer, NIBGE, Faisalabad. The text/specifications of the tenders can also be downloaded on PPRA website www.pprs.org.pk and NIBGE Website www.nibge.org.pk. The Separate envelope procedure for technical and financial proposals must be adapted.

Sr. Admin. Officer (Procurement)


NIBGE, P.O.Box # 577, Jhang Road, Faisalabad. Ph: (041) 2651475-79/Ext. 201,206/(041) 2650818

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD:

Tender No.22/2010
Note:-

Date of opening 03-09-2010 at 1300 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications of Chemicals for purchase out of PARB Wheat Project No 337


Project title: Improvement of salt tolerance in wheat through biotechnology Following materials are required for research project requiring high quality molecular biology and plant tissue culture grade materials. The standardized specifications of the materials meet the product and performance specifications of 36 manufacturers including; 1) Invitrogen 2) Clontech 3) Sigma 4) Aldrich 5) Fermentas 6) Genomed 7) Roth 8) Stratagene 9) GE Healthcare 10) Roche 11) MRC 12) Phytotechnology 13) Promega 14) Bio-Rad 15) Novagen 16) Ambion 17) MWG/Operon 18) US Biologicals 19) Qiagen 20) NEB 21) Research Organics 22) Riedel-deHaen 23) Gene Link 24) Eppendorf 25) Axygen 26) Naptune 27) Bio Plastic 28) Nalgene 29) Nunc 30) Labnet 31) Greiner 32) Nerbe Plus 33) Corning 34) CLP 35) NyxTechnik 36) Oxoid The quotes for materials from only above mentioned manufacturers may be submitted. The quality of the products and time of delivery per quote is very important and not compromise-able. All the perishable enzymes/materials should be assured for transport in dry ice from the point of origin till those reach the end user. The commodities requiring refrigeration must be transported in ice packs and reach the end user without suffering a heat shock. Humble request to meet these requirements is extended to avoid any later issues as the payment will be released after quality assurance that may take up to 30 days time. Note: Only sole/authorized agents of the above mentioned companies should participate and certificate must be presented at the time of quote. Cat # must be mentioned with the quote for verification. Import documents must be presented at the time of delivery. MB stands for Molecular Biology Grade and DNase/RNase free. Plant Cell Culture Tested (PCCT means the Ultra Purity Grade available to date). Sole/authorized agents must be registered with any Punjab academic / Research institute
Name of Item 1. Isopropanol: 2-Propanol, Isopropyl Alcohol, (CH3)2CHOH, structural isomer of propanol (Molecular Biology Grade), Purity 99% Qty 4L

2. Absolute Ethanol: (Molecular Biology Grade), Purity 99%, (CH3CH2OH) 3. Acetic Acid: (Molecular Biology Grade), Purity 99%, (CH3COOH) 4. Low electroendosmosis agarose (LE Agarose) with following specifications: EEO (mr) 0.12, Gelling Temp (1.5%) 35C +/- 1.5, Melting Point 89C +/- 1.5, Gel Strength (1%) 1800 gm/cm2, Moisture <6%, Sulfate <0.2%, Ash <0.2% Quality Control: Should not have nonspecific Endonuclease, Exonuclease or RNAse activity. Must be suitable for separation, elution and ligation of 100 bp to 30Kbp DNA fragments. Storage Conditions: Powder stable at room temperature. 5. Ammonium Acetate: (Molecular Biology Grade) 98% Pure Dnase/Rnase Free (CH3COONH4) FW 77.08 6. Ammonium Nitrate: (NH4NO3) FW 80.04, Plant Tissue Culture Tested (PTCT) Ultra Pure, Purity 99% 7. Ammonium Per Sulphate: (Molecular Biology Grade) Min 98% purity level, (NH4)2S2O8 FW 228.2 8. Ammonium Sulphate: (NH(4)2SO4) FW 132.14, Plant Tissue Culture Tested (PTCT), Purity 99% 9. Ascorbic Acid: (C15H20O4) FW 264.32, Plant Tissue Culture Tested (PTCT), Min Purity 99% 10. Boric Acid: Formula (H3BO3), FW 61.83, Plant Tissue Culture Tested (PTCT), Purity 99% 11. Bromo Phenol Blue: (Molecular Biology Grade), Formula (C19H10Br4O5S), FW 670.0 12. Calcium Chloride Dihydrate: CaCl2.2H2O FW 147.02 Plant Tissue Culture Tested (PTCT) Purity 99% 13. Calcium Pantothanate: (C18H32O10N2Ca) FW 476.54, Plant Tissue Culture Tested (PTCT) Purity 99% 14. Carbenic disodium salt: Carbenicillin (alpha-carboxybenzylpenicillin) is a synthetic penicillin derivative used as a selection agent for carbenicillin-resistant plasmids, (Molecular Biology Grade) C17H16N2O6SNa2, Mol Wt 422.4, 15. Casein Hydrolysate: A preparation made from the milk protein casein, which is hydrolyzed to break it down into its constituent amino acids, Plant Tissue Culture Tested (PTCT) Purity 99% 16. Chloroform: (CHCl3), Purity 99+%, Required for nucleic acid purification. FW 119.4 17. Cobalt Chloride Hexahydrate: (CoCl2.6H2O) FW 237.84, Plant Tissue Culture Tested (PTCT) 18. CTAB Cetyl trimethyl ammonium bromide: (Molecular Biology Grade) Purity 98% (C19H42BrN) FW 364.5, required for DNA Isolation. 19. Cupper Sulphate Pentahydtrate: (CuSO4.5H2O) FW 249.68, Plant Tissue Culture Tested (PTCT) 20. DEPC Dietylpyrocarbonate:

5L 5L 500g

250g 1Kg 1Kg 500g 25g 500g 25g 2Kg 25 g 25g

100g

5L 25g 1Kg 250g 400ml

(Molecular Biology Grade), Formula O(COOC2H5), Mol Wt 162.1, NMR Purity 97% 21. Dextran Sulphate: (MW > 500000), (C18H32O16), H-Bond Donor 11,H-Bond Acceptor 16 (Molecular Biology Grade) 22. Difco/Bactoagar: (Molecular Biology Grade) Clarity,1.5%,Gelation Point (C) 35C, Melting Point (C) 88C 23. DMSO Dimethyl sulfoxide: Purity 99% (Molecular Biology Grade) (CH3)2SO, Mol Wt 78.13 24. EDTA Ethylenediamine-N,N-diacetic acid: (Molecular Biology Grade) Purity 99% (C10H16N2O8) FW 292.2 25. Ethidium Bromide: (Molecular Biology Grade) Purity 98% (C21H20N3Br) FW 394.3 26. Fe-EDTA Ferrous Sulphate Chelate Sol Contains 2.785 g/L FeSO4 7H2O and 3.725g/L EDTA 2Na 2H2O, sterilefiltered, 100 concentration, plant cell culture tested. 27. Ferrous Sulphate Heptahydrate: Formula (FeSO4.7H2O), FW 278.02, Plant Tissue Culture Tested (PTCT) 28. Formamide: (Molecular Biology Grade) Purity 99.%, Formula (CH3NO) FW 45 29. Glucose Anhydrous: (Molecular Biology Grade) (C6H12O6) FW 198.2 Purity 99% 30. Glutamine 2,5 Diamino-5-Oxopentanoic Acid: FW 146.14, Plant Tissue Culture Tested (PTCT), Purity 99% 31. Glycerol: (Molecular Biology Grade), Formula (C3H8O3) FW 92.09 Purity 99% 32. Glycine: (MB Grade), Formula (C2H5NO2) FW 75.07 Purity 99% 33. IPTG Isopropyl -D-1-thiogalactopyranoside: (Molecular Biology Grade), Formula (C9H18O5S), FW 238.3 Purity 99% 34. Isoamyl Alcohal: (Molecular Biology Grade), Formula (C5H12O) FW 88.15 Purity 99% 35. Kanamycine Monosulphate (Molecular Biology Grade), Formula (C18H36N4O11.H2SO4) FW 582.6 Purity 99% 36. Lysozymes: (Molecular Biology Grade) Salt Free, Albumin Free, Lypholized 37. Magnesium Chlorode Hexahydrate: (Molecular Biology Grade), Formula (MgCl2.6H2O) FW 203.3 Purity 99% 38. Magnesium Sulphate Anhydrous (Molecular Biology Grade), Formula (MgSO4) FW 120.4 Purity 99% 39. Magnisium Sulphate Heptahydrate (Molecular Biology Grade), Formula (MgSO4.7H2O), FW 246.48, Plant Tissue Culture Tested (PTCT) 40. Maltose Monohydrate: (Molecular Biology Grade), Formula (C12H22O11.H2O) FW 360.32, Plant Tissue Culture Tested (PTCT) 41. Mercaptoethanol: (Molecular Biology Grade), Formula (C2H6OS), FW 78.13 42. MES 2-(N-Morpholino)Ethanesulphonic Acid (Molecular Biology Grade), Formula (C6H13NO4S), FW 195.2, Plant Tissue Culture Tested (PTCT) 43. MOPS 3-(N-morpholino)propanesulfonic acid (Molecular Biology Grade), Formula (C7H14NO4SNa) FW 231.2 Purity 99%

500 g 2Kg 2.5L 1Kg 5g 100g 250g 1L 5Kg 250g 2.5L 500g 5g 2L 5g 1g 500g 500g 2Kg 3Kg 250ml 500g 100g

44. MS Salt with vitamins: Plant Tissue Culture Tested (PTCT), Purity 99% 45. Myoinosotol: (Molecular Biology Grade), Formula (C8H12O6) FW 180.16, Plant Tissue Culture Tested (PTCT) 46. Nicotinic Acid: (Molecular Biology Grade), Formula (C6H5NO2) FW 123.12 Plant Tissue Culture Tested (PTCT) Purity 99% 47. Phenol chloroform: (Molecular Biology Grade), ready to use with pH 7.00-7.8 48. Polyvinylpyrrolidone PVP: (Mol Wt 40000), Molecular Biology Grade, Plant Tissue Culture Tested (PTCT) 49. Potassium Acetate: (Molecular Biology Grade), Formula (CH3COOK) FW 98.14 Purity 99% Dnase/Rnase Free 50. Potassium Chlorode: (Molecular Biology Grade), Formula (KCl) FW 74.55 Purity 99% 51. Potassium Hydroxide (Molecular Biology Grade), Formula (KOH) FW 56.11 Purity 85% 52. Potassium Iodide: (Molecular Biology Grade), Formula (KI) FW 166.00, Plant Tissue Culture Tested (PTCT) 53. Potassium Nitrate: (Molecular Biology Grade), Formula (KNO3) FW 101.10, Plant Tissue Culture Tested (PTCT) 54. Potassium Phosphate Monobasic: (Molecular Biology Grade), Formula KH2PO4 FW 136.09 Plant Tissue Culture Tested (PTCT) 55. Protenase K: (Molecular Biology Grade) Nuclease Free 56. Pyridoxine-Hydrochloride: (Molecular Biology Grade), Formula (C8H11NO3.HCL), FW 205.64, Plant Tissue Culture Tested (PTCT) 57. Rifampicin: (Molecular Biology Grade), Formula (C43H58N4O12), FW 822.94, Plant Tissue Culture Tested (PTCT) 58. Sodium Acetate: (Molecular Biology Grade), Formula (CH3COONa) FW 82.03 Purity 99% 59. Sodium Chlorode: (Molecular Biology Grade), Formula (NaCl), DNase, RNase, and protease 60. Sodium Citrate: (Molecular Biology Grade), Formula (C6H5O7Na3-2H2O) Mol.Wt 294.1 Purity 99% 61. Sodium Dodycyl Sulphate SDS (Molecular Biology Grade), (Mol 288.38) Purity 99% 62. Sodium Hydroxide Anhydrous (Molecular Biology Grade), Formula (NaOH), FW 40 Purity 99% 63. Sodium Molybdate: (Molecular Biology Grade), Formula (Na2MoO4.2H2O), FW 241.95, Plant Tissue Culture Tested (PTCT) 64. Sodium Phosphate: (Molecular Biology Grade), Formula (NaH2PO4.H2O)FW 137.99, Purity 99% 65. Spermidine Formula (C7H19N3) FW 145.25, Plant Tissue Culture Tested (PTCT)

10Packs 500g 100g 400ml 500g 500g 500g 250g 100g 5Kg 1Kg 5ml 25g 10g 1Kg 500g 500g 1Kg 1Kg 100g 1Kg 5g

66. TEMED Tetramethylethylenediamine:


(Molecular Biology Grade), Formula C6 H16N2) Mol Wt 116.2 Purity 99% 67. Thiamine-Hydrochlorode: (MB Grade), Formula (C12H17CIN4OS.HCL) FW 337.27 Plant Tissue Culture Tested (PTCT) 68. Tris-Base 2Amino-2hydroxymethyl-1,3propanediol: (Molecular Biology Grade), Formula C4H11NO3 FW 121.13, Purity 99% 69. Tris-HCl (MB): Formula (C4H11NO3-HCL) Mol Wt 157.6 Purity 99% Nuclease Free 70. Tryptone: (Molecular Biology Grade), Powder is homogeneous, free-flowing and light beige to tan, pH 6.8 - 7.3 71. Xgal 5bromo-4chloro-3indolyl--D-galactopyranoside: (Molecular Biology Grade), Formula (C14H15BrCINO6), FW 408.63, Plant Tissue Culture Tested (PTCT) 72. X-Gluc, 5-Bromo-4-chloro-3-indolyl b-D-glucuronide: (Molecular Biology Grade), C14H12BrClNO7Na FW 444.59 Purity 99% 73. Yeast Extract: (Molecular Biology Grade) Powder should be homogeneous, free-flowing, and light beige to tan. 74. Zinc Sulphate Heptahydrate: (Molecular Biology Grade), Formula ZnSO4.7H2O, FW 287.54, Plant Tissue Culture Tested (PTCT)

100ml 25g 2Kg 1Kg 1Kg 1g 500mg 1Kg 500g

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

Made in: USA, Europe or Japan only 2. The soft copy of the financial quotation should also be provided on a W/RW CD along with your financial quotation please.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD:

Tender No.23/2010
Note:-

Date of opening 06-09-2010 at 1200 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications of Consumables/Kits for purchase out of PARB Wheat Project No. 337
Project title: Improvement of salt tolerance in wheat through biotechnology. Following materials are required for research project requiring high quality molecular biology and plant tissue culture grade materials. The standardized specifications of the materials meet the product and performance specifications of 36 manufacturers including; 1) Invitrogen 2) Clontech 3) Sigma 4) Aldrich 5) Fermentas 6) Genomed 7) Roth 8) Stratagene 9) GE Healthcare 10) Roche 11) MRC 12) Phytotechnology 13) Promega 14) Bio-Rad 15) Novagen 16) Ambion 17) MWG/Operon 18) US Biologicals 19) Qiagen 20) NEB 21) Research Organics 22) Riedel-deHaen 23) Gene Link 24) Eppendorf 25) Axygen 26) Naptune 27) Bio Plastic 28) Nalgene 29) Nunc 30) Labnet 31) Greiner 32) Nerbe Plus 33) Corning 34) CLP 35) NyxTechnik 36) Oxoid The quotes for materials from only above mentioned manufacturers may be submitted. The quality of the products and time of delivery per quote is very important and not compromise-able. All the perishable enzymes/materials should be assured for transport in dry ice from the point of origin till those reach the end user. The commodities requiring refrigeration must be transported in ice packs and reach the end user without suffering a heat shock. Humble request to meet these requirements is extended to avoid any later issues as the payment will be released after quality assurance that may take up to 30 days time. Note: Only sole/authorized agents of the above mentioned companies should participate and certificate must be presented at the time of quote. Cat # must be mentioned with the quote for verification. Import documents must be presented at the time of delivery. MB stands for Molecular Biology Grade and DNase/RNase free. Plant Cell Culture Tested (PCCT means the Ultra Purity Grade available to date). Sole/authorized agents must be registered with any Punjab academic / Research institute Name of |Item Qty 1. DIG DNA Labeling and Detection kit (10-15 reactions kit) 1Kit Random primed DNA labeling with digoxigenin-dUTP, alkali-labile and chemiluminescence detection with CSPD, ready-to-use. DIG-labeled DNA probes should be generated with DIG-High Prime according to the random primed labeling technique.

2. 3.

4.

5.

6.

7.

DIG-High Prime should develop reaction mixture containing digoxigenindUTP, alkali-labile and all reagents, including enzyme necessary for random primed labeling, premixed in an optimized 5 concentrated reaction buffer. Should be used for all types of filter hybridization. Should be used for single copy gene detection in total genomic DNA, even from organisms with high complexity, e.g. human, barley, and wheat. DIG-labeled probes must be capable of hybridizing to membrane blotted nucleic acids and easily re-hybridize with a second DIG-labeled probe. The hybridized probes should be immunodetected with anti-digoxigenin-AP, and visualized with the chemiluminescence substrate CSPD. Hybridization solutions should be reused 3 5 times. DIG-labeled probes should be stored for at least one year. DNase, Rnase Free, Strength 1u/l Required for digestion of single and double stranded DNA and preparation of DNA free RNA prior to RT-PCR. ApaI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. BamHI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. ClaI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. EcoRI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. HindIII (10 u/ul)

1000 units 3000 units

4000 units

600 units

5000 units

5000

The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 8. KpnI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 9. PstI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 10. SacI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 11. SnaBI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 12. SpeI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using

units

4000 units

3000 units

1200 units

600 units

400 units

catalogue number, which should be clearly provided. 13. XbaI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 14. XhoI (10 u/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 15. Conventional Restriction Enzyme XmaI 300 Units Strength 10u/l The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 16. Gene Ruler 1Kb DNA Ladder Ready to Use, premixed with 6x DNA loading dye; Conc. 0.5 ug/ul 17. Gel Red (DNA Staining Dye, non carcinogenic, biologically safe and easy to handle) 18. Plant Genomic DNA purification kit Required for rapid and high quality plant genomic DNA purification. The kit should utilize a silica based membrane technology in the form of a spin column. The purified DNA would be up to 50 kb in size. Purified DNA could be utilized for Southern PCR and other downstream applications. 19. Glycogen (Highly pure, DNA/ RNA Free, Molecular Biology Grade. Conc. 20mg/ml aqueous solution. Required for precipitation of nucleic acids 20. RNAase Inhibitor (MB grade), Inhibit activity of RNase A, B, C. One unit should inhibit the activity of 50ng RNase by 50%. 21. Plant RNA Extraction Reagent Required for isolation of high quality total RNA from plant tissues. The volume supplied should be sufficient for 200 reactions using 100mg tissues and give high yield. Reagent should contain b-mercaptoethanol and sodium azide. Isolated RNA must of high quality than can be used for Northern blot, cDNA synthesis and other applications.

1500 units

2000 units

300 units

500 Applic . 10000 App. 1Kit x 250 reacti ons 4 Vials 2500 units (40U/ l) 100 ml

22. RNase (DNase Free) RNase required for degradation of RNA. Conc. 10mg/ml, Applications in plasmid and genomic DNA purifications 23. Salmon Sperm DNA (Single stranded). Molecular Biology Grade. Conc. 10mg/ml; Use as blocking agent in Southern/Northern blot. 24. Trizol Reagent: (DNA/RNA and Protein Extraction) Ready to use and suitable for isolating total RNA, genomic DNA and proteins from variety of tissues and cell types in single prep or in separate prep. 25. cDNA synthesis kit: (100 reactions kit) The First Strand cDNA Synthesis Kit should provide a complete system for efficient synthesis of first strand cDNA from RNA templates. It should be suitable for synthesis of cDNA from 10 to 13 kb. The Reverse Transcriptase included in the kit must be completely devoid of RNase H activity associated with the enzyme to avoid RNA degradation in RNADNA hybrids during synthesis of the first strand cDNA. The enzyme should be capable of maintaining activity over a wide temperature range (42-55C), for efficient reverse transcription of RNAs having a high degree of secondary structure. The RNAse inhibitor should be provided in the kit to effectively protect RNA template from degradation. The kit must also include both oligo(dT) and random hexamer primers to support cDNA sunthesis from RNAs with or without poly(A) tail. 26. dNTPs; 100mM each (not mixed) 4 x 0.25 ml (4 x 25 mol of 100 mM solution). Greater than 99% pure and confirmed by HPLC. Free of human and E.coli DNA. Should be highly stable at neutral pH. The stability should meet a minimum of 100 freezethaw cycles. 90-95% of dNTPs should remain in triphosphate form even after 7 weeks at room temperature. 85-90% of dNTPs should remain in triphosphate form after 30 cycles of PCR (1 min at 94C; 3 min at 72C). Designed especially for high efficiencies in long range PCR (40 kb), cDNA synthesis, RT-PCR, real-time PCR, standard PCR, and high fidelity PCR. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. 27. Gel elution Kit (250 preps) Silica membrane based minispin cartridges and associated solutions (gel melting, column wash, DNA elution) capable of 95% recoveries in the range of 100 bp 10 kb DNA fragments and supplied with binding buffer, concentrated wash buffer, elution buffer, purification columns, collection tubes and detailed protocol. The eluted DNA should be pure enough for restriction digestion, DNA sequencing, DNA ligation and labeling.

10 Vials 10 Vials 500ml

1Kit

4 Set

1Kit

28. PCR Purification Kit (250 preps) 1 Kit PCR Purification Kit for rapid and efficient purification of DNA from PCR and other enzymatic reaction mixtures. Silica membrane based spin columns capable of 90-100% recoveries in the range of 100 bp 10 kb and supplied with binding buffer, concentrated wash buffer, elution buffer, purification columns, collection tubes and detailed protocol. The eluted DNA should be pure enough for restriction digestion, DNA sequencing, DNA ligation and labeling. 29. Miniprep Plasmid Isolation kit (250 preps) 1 Kit

Efficient silica membrane based spin columns system for the isolation of high quality plasmid DNA from recombinant E.coli cultures. The yield should be up to 20 g of high quality plasmid DNA per 3 ml culture. The kit should be provided with Resuspension solution, Lysis solution, Neutralization solution, Wash solution, RNAse solution, Elution buffer, Silica membrane based spin columns, Collection tubes and detailed protocol. The isolated plasmid DNA should be pure enough for restriction digestion, DNA sequencing, DNA ligation and labeling. 30. SYBR Green/Fluorescein qPCR Master Mix (2X); (200 reactions) 2 x 1.25 ml (for 200 react. of 25 l each). Ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR with the Bio-Rad iQ machine. The master mix should include Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. The reaction mix should contains SYBR Green I dye supplemented with fluorescein passive reference dye. Only template and primers should be required. The fluorescein included in the master mix should allow for the collection of Dynamic Well Factors with the Bio-Rad iQ5 machine, but should not affect qPCR efficiency. The dUTP should be included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The nuclease free water should also be a component of the kit for volume adjustments. 31. T4 DNA Ligase: 1000 Weiss Units (5 Weiss units/l) The enzyme should catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme must be capable of repairing single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. It should efficiently join DNA fragments with either cohesive or blunt termini, but should not have such activity on single-stranded nucleic acids. The enzyme should remain 100% active in restriction enzyme, PCR and RT buffers, supplemented with ATP. One unit (Weiss unit) of the enzyme should catalyze the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37C. There should be no endo-, exodeoxyribonucleases, phosphatases and ribonucleases activities associated with the enzyme and the quality tests on the enzyme must be there on the product insert. The enzyme should be supplied with 10X T4 DNA Ligase Buffer. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. 32. TA Cloning Kit: (30RXN) Thermostable with half life more than 40 min at 95C. Should generate PCR products with 3-dA overhangs and supplied with two buffers 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4 for testing at wide range of magnesium concentrations and decreases unspecific priming. The 25mM MgCl2 should also be supplied. The enzyme should be capable of incorporating modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides). For routine PCR the amplification of DNA fragments up to 5 kb should be possible. The enzyme should catalyzes 5' to 3' synthesis of DNA with no detectable 3' to 5' exonuclease (proofreading) activity. It should possess negligible or low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase should exhibit deoxynucleotidyl transferase activity to add extra adenines at the 3'-end of PCR products. One unit of the enzyme should catalyze the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide

1Kit

10 Vials

2Kits

fraction in 30 min at 70C. The error rate of Taq DNA Polymerase should not be more than 2.2x10-5 errors per nt per cycle, and the accuracy of PCR should be 4.5x104. The enzyme must be devoid of E.coli DNA and non-specific amplifications must not be obtained in a template free PCR. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. 33. Taq DNA Polymerase (recombinant), 500 Units (5 units /l) 20 Thermostable with half life more than 40 min at 95C. Should generate Vials PCR products with 3-dA overhangs and supplied with two buffers 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4 for testing at wide range of magnesium concentrations and decreases unspecific priming. The 25mM MgCl2 should also be supplied. The enzyme should be capable of incorporating modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides). For routine PCR the amplification of DNA fragments up to 5 kb should be possible. The enzyme should catalyzes 5' to 3' synthesis of DNA with no detectable 3' to 5' exonuclease (proofreading) activity. It should possess negligible or low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase should exhibit deoxynucleotidyl transferase activity to add extra adenines at the 3'-end of PCR products. One unit of the enzyme should catalyze the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70C. The error rate of Taq DNA Polymerase should not be more than 2.2x10-5 errors per nt per cycle, and the accuracy of PCR should be 4.5x104. The enzyme must be devoid of E.coli DNA and non-specific amplifications must not be obtained in a template free PCR. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. Made in: USA, Europe or Japan only
Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

2. The soft copy of the financial quotation should also be provided on a W/RW CD along with your financial quotation please.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.24/2010 Note:Date of opening 07-09-2010 at 1200 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications of Chemicals/Kits for purchase out of PARB Wheat Project No. 103
Project title: Wheat Crop Improvement for drought tolerance through biotechnology Following materials are required for research project requiring high quality molecular biology and plant tissue culture grade materials. The standardized specifications of the materials meet the product and performance specifications of 36 manufacturers including; 1) 4) 7) 10) 13) 16) 19) 22) 25) 28) 31) 34) Invitrogen Aldrich Roth Roche Promega Ambion Qiagen Riedel-deHaen Axygen Nalgene Greiner CLP 2) 5) 8) 11) 14) 17) 20) 23) 26) 29) 32) 35) Clontech Fermentas Stratagene MRC Bio-Rad MWG/Operon NEB Gene Link Naptune Nunc Nerbe Plus NyxTechnik 3) 6) 9) 12) 15) 18) 21) 24) 27) 30) 33) 36) Sigma Genomed GE Healthcare Phytotechnology Novagen US Biologicals Research Organics Eppendorf Bio Plastic Labnet Corning Oxoid

The quotes for materials from only above mentioned manufacturers may be submitted. The quality of the products and time of delivery per quote is very important and not compromise-able. All the perishable enzymes/materials should be assured for transport in dry ice from the point of origin till those reach the end user. The commodities requiring refrigeration must be transported in ice packs and reach the end user without suffering a heat shock. Humble request to meet these requirements is extended to avoid any later issues as the payment will be released after quality assurance that may take up to 30 days time. Note: Only sole/authorized agents of the above mentioned companies should participate and certificate must be presented at the time of quote. Cat # must be mentioned with the quote for verification. Import documents must be presented at the time of delivery. MB stands for Molecular Biology Grade and DNase/RNase free. Plant Cell Culture Tested (PCCT means the Ultra Purity Grade available to date). Sole/authorized agents must be registered with any Punjab academic / Research institute.

Name of |Item 1. 1-Naphthalene acetic acid (NAA): Formula: C12H10O2, FW: 186.2, Plant Cell Culture Tested, Storage Temp: 2-8 C, Solubility: acetone: 50mg/ml, crystalline, light yellow, Purity: 99%. 2. Carbencillin, -carboxybenzylpenicillin, disodium salt: Plant Cell Culture Tested, Formula: C17H16N2O6SNa2, FW: 422.41, Storage: 2-6 C, water soluble, Whit to off-white powder, water soluble. 3. Murashige & Skoog (MS) Basal Medium with macro and micro nutrients, and Vitamins as described by Murashige & Skoog (1962): Ammonium Nitrate: 37.21 %, Potassium Nitrate: 42.85 %, EDTA, Disodium Salt, Dihydrate: 0.84 %, Cobalt Chloride, Hexahydrate: 0.0006 %, Cupric Sulfate, Pentahydrate: 0.0006 %, Sodium Molybdate(VI), Dihydrate: 0.006 %, Manganese Sulfate, Monohydrate: 0.38 %, Potassium Iodide: 0.019 %, Boric Acid: 0.14 %. Plant Cell Culture Tested, Storage Temp: 2-6 C, pH: 3.5 - 4.5, soluble in water, off-white to yellow powder, odorless. 4. Indole-3-Acetic Acid (IAA): formula: C10H9NO2, FW: 175.2, Plant Cell Culture Tested, purity: 99%, off white to tan crystals. 5. RNAase Inhibitor (Molecular Biology grade), Inhibit activity of RNase A, B, C. One unit should inhibit the activity of 50ng RNase by 50%. 6. IPTG: (Molecular Biology Reagent), purity 99%, Molecular Formula: C9H18O5S; Molecular mass: 238.3 g mol1 ; Isopropyl-B-Dthiogalactopyranoside, dioxane free, IPTG is required for induction of transcription of the gene coding for beta-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of -galactosides into monosaccharides. blue white selection 7. X-Gal (Molecular Biology grade, 99% pure), 5-Bromo-4-Chloro-3-IndolyleB-D-Glactosidase, C14H15BrCINO6, MW 408.64; Suitable for blue white selection. Required for differentiation of recombinants from nonrecombinants in cloning experiments using vectors containing the lacZ or lacZ a-peptide gene. 8. Chloroform: (Molecular Biology grade): Purity 99+%, Required for nucleic acid purification. 9. Ethanol: Absolute 99.5 % (Molecular Biology grade).Required for DNA precipitation 10. Isoamylalcohol: Molecular Biology grade, purity 98%, Required for nucleic acid purification 11. Ampicillin:, Antibiotic; D(-)-a-Aminobenzylpenicillin sodium salt (Molecular Biology grade-required for plasmid selection). Formula: C16H18N3NaO4S; FW: 371.4. water soluble. 12. Rifampicin: Antibiotic; Plant Cell Culture Tested); Formula: C43H58N4O12; MW: 822.9; Required for effective killing of bacteria. 13. Sucrose: (Plant Cell Culture Tested), D(+)-Sucrose, 99+% pure, RNase, DNase free (FW=C12 H22O11), Soluble in water @ 1970g/l ( at15oC). 14. MES: 2-(N-Morpholino)ethanesulfonic Acid; Plant Cell Culture Tested, Formula: C6H13NO4S; FW:195.2; white crystalline powder; purity > 98% , free acid, monohydrate crystalline, pKa = 6.15 at 25 oC. 15. Kinetin: 6-Furfurylaminopurine; C10H9N5O,Plant Cell Culture Tested, White to Yellow Crystalline Powder, MW: 215.21 16. BAP: 6-BENZYLAMINOPURINE, C12H11N5; Plant Cell Culture Tested,

Qty 1 x 25 g 2 x 5g 10 packs ( 50 liters each)

1 x 5g 2500 units (40U/l) 10 g

2g

2 x 2.5 L 2 x 2.5 L 1 x 2.5 L 1 x 100 g 1 x 5g 5 x 5kg 1 x 500 g 1 x 5g 1x5g

98%, MW: 225.25; White to off white Powder. 17. 2,4-D: 2-4-Dicholorophenoxyacetic acid, C8H6Cl2O3; Plant Cell Culture Tested, Purity 98%, Crystalline white to light yellow, MW:221.04; 18. Tris-HCL (Tris hydrochloride): Molecular Biology grade, (C4H11NO3HCL) Mol. wt. 157.6 Purity 99% Nuclease Free, White Crystalline Powder, pH 7-9, pKa:8.1 (25C), Storage: Room temperature, Heavy Metals 5 PPM. 19. Phytagel ( or Gellan Gum or Gelrite): Gelling agent, Plant Cell Culture tested Powder, Strength 550-850 g/cm2, Provide clear, colorless and high strength gel. Loss on drying ~ 15.0 %. Gel structure should support plant cells and microbial growth. Polysaccharide comprising glucoronic acid, rhamnose and glucose. 20. DIG DNA Labeling and Detection System Random primed DNA labeling with digoxigenin-dUTP, alkali-labile and chemiluminescence detection with CSPD, ready-to-use.DIG-labeled DNA probes should be generated with DIG-High Prime according to the random primed labeling technique. DIG-High Prime should develop reaction mixture containing digoxigenindUTP, alkali-labile and all reagents, including enzymenecessary for random primed labeling, premixed in an optimized5 concentrated reaction buffer. Should be used for all types of filter hybridization. Should be used for single copy gene detection in total genomic DNA, even from organisms with high complexity, e.g. human, barley, and wheat. DIG-labeled probes must be capable of hybridizing to membrane blotted nucleic acids and easily re-hybridize with a second DIG-labeled probe. The hybridized probes should be immunodetected with anti-digoxigenin-AP, and visualized with the chemiluminescence substrate CSPD. Hybridization solutions should be reused 3 5 times. DIG-labeled probes should be stored for at least one year. Manufactured in: USA, Europe or Japan only

1 x 250 g 500g

1x5 Kg

2 Kit (12 reactio ns/ kit)

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

2. The soft copy of the financial quotation should also be provided on a W/RW CD along with your financial quotation please.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.25/2010 Note:Date of opening 08 -09-2010 at 1200 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications of Glassware/plasticware for purchase out of PARB Wheat Project No. 337
Project title: Improvement of salt tolerance in wheat through biotechnology. Following materials are required for research project requiring high quality molecular biology and plant tissue culture grade materials. The standardized specifications of the materials meet the product and performance specifications of 36 manufacturers including; 1) Invitrogen 2) Clontech 3) Sigma 4) Aldrich 5) Fermentas 6) Genomed 7) Roth 8) Stratagene 9) GE Healthcare 10) Roche 11) MRC 12) Phytotechnology 13) Promega 14) Bio-Rad 15) Novagen 16) Ambion 17) MWG/Operon 18) US Biologicals 19) Qiagen 20) NEB 21) Research Organics 22) Riedel-deHaen 23) Gene Link 24) Eppendorf 25) Axygen 26) Naptune 27) Bio Plastic 28) Nalgene 29) Nunc 30) Labnet 31) Greiner 32) Nerbe Plus 33) Corning 34) CLP 35) NyxTechnik 36) Oxoid The quotes for materials from only above mentioned manufacturers may be submitted. The quality of the products and time of delivery per quote is very important and not compromisable. All the perishable enzymes/materials should be assured for transport in dry ice from the point of origin till those reach the end user. The commodities requiring refrigeration must be transported in ice packs and reach the end user without suffering a heat shock. Humble request to meet these requirements is extended to avoid any later issues as the payment will be released after quality assurance that may take up to 30 days time. Note: Only sole/authorized agents of the above mentioned companies should participate and certificate must be presented at the time of quote. Cat # must be mentioned with the quote for verification. Import documents must be presented at the time of delivery. MB stands for Molecular Biology Grade and DNase/RNase free. Plant Cell Culture Tested (PCCT means the Ultra Purity Grade available to date). Sole/authorized agents must be registered with any Punjab academic / Research institute Name of |Item Qty 1. Beaker (Pyrex or equivalent) (2L, 1L, 500ml, 250ml,100ml) 3 Autoclavable, capable of tolerating high temperature, pressure and Each corrosive chemicals. 2. Blue or White Tips (0.01ml-1ml), 10 should accurately fit Gilson, Eppendorf and BioHit pipetman Pack

3. Electroporator Cuvetts Gap width 0.1cm and 0.2cm. Should fit BTX electrporator for bacterial transformations 4. Enzyme Storage Boxes Requires for protection of enzymes and temperature sensitive reagents during transport and storage. Remain cool for 2-3 hours. Contain nontoxic cooling gel. 20 places polycarbonate block for 1.5 ml tubes, Temp -10 to -20 C. clear non-filled lid. 5. Face Mask: To protect digestive and respiratory track for inhalation of corrosive materials 6. Falcon Tubes (15ml), Sterilized, DNase, RNase Free Approximate dimensions: 16 mm O. D.; 115 mm length, sterilized by gamma irradiation and non-pyrogenic, dark color printed graduations and white writing patch, high-density polyethylene flat-top screw cap to allows one hand manipulation, provides a level writing area. Modified polystyrene to improved stress resistance. The top of the cap must have a frosted writing surface for labeling. RNase and DNase free 7. Falcon Tubes (50ml), Sterilized, DNase, RNase Free Approximate dimensions: 30 mm O. D.; 115 mm length, sterilized by gamma irradiation and non-pyrogenic, dark color printed graduations and white writing patch, high-density polyethylene flat-top screw cap to allows one hand manipulation, provides a level writing area. Modified polystyrene to improved stress resistance. The top of the cap must have a frosted writing surface for labeling. RNase and DNase free 8. Conical Flasks (Pyrex or equivalent) 1L, 2L, 500ml, 250 ml 9. Measuring Cylender (Transparent Plastic with legible measuring points, autoclavable) 2L, 1L, 500ml, 250ml, 100ml 10. Parafilm Roll 4" x 125Ft It should stretch up to 200 % and cling (seal) around laboratory vessels on even and uneven surfaces. Made of disposable, non-toxic translucent material mainly polyolefins and paraffin waxes. Good gas and water permeability. It should withstand saline, alkaline and inorganic solutions for 48 hours. Withstand temperature from -45 0C to +45 0C 11. Strips of eight PCR Tubes (0.2ml), thin walled, DNase/RNase Freee, Temp Range -4C to 121C with cap strips 12. Disposable and sterile plastic petriplates Disposable and sterile plastic petriplates for plant tissue culture, size 90-100mm (dia) and 15-16mm deep, ; 20 per pack. Quantity 2,000 plates 13. Storage Boxes (Plastic with lid/cap for 1.6ml eppendorf tubes) Polycarbonate boxes with 80-100 places for 1.5 ml tubes for storage in freezer. 14. Storage Racks (Made of steel or any other metallic material to keep in

s 50 Each 2 B ox es 10 500 Tube s

700 Tube s

5 Each 3 Each 5 Rolls

10 Pack s 2000 Plate s 100 Boxe s 20

order storage boxes of 1.6ml eppendorf storage boxes) with handle on the top. Height 18-20 inches that can hold 9-10 boxes of 2H. 15. Filtered Tips, Pre Sterlized, Cap .1l-10l should accurately fit Gilson, Eppendorf and BioHit pipetman 16. Dewar Flask for liquid nitrogen (287X146mm) Temp Range -196 to 100C with and handel Cap 4L 17. Syringe Filter 0.2m pore, diameter 33mm. Required for filter sterilization of antibiotics and other heat labile chemicals. Membrane made of Polyvenylidene fluoride (PVDF), sterile, single packed. 18. Sterile Filteration System Capacity 250ml, Accepts 47mm filters, Autoclaveable 19. 1.6ml Eppendorf tubes: 1.6 ml Eppendorf tubes. Clear polypropylene, autoclavable. Unbreakable at 14,000 rpm/16,000 rcf. The tubes should withstand liquid nitrogen. The tubes should not break when spun after liquid nitrogen treatment. The lid/cap should provide enough grip surface for easy opening/closing of tubes at ultra low temperature. Frosted lid surface for writing. Calibrations for volume on the tube surface should be engraved or printed. Required in Bulk Pack. 20. PCR Tubes (0.2ml) 0.2 ml thin walled autoclavable and clear polypropylene PCR tubes, optically transparent cap/lid, required in bulk pack. Quantity: 4,000 tubes 21. Ridged 10 l tips, Clear polypropylene and autoclavable,, should accurately fit Gilson, Eppendorf and BioHit pipetteman. Required in bulk pack. Quantity: 5,000 tips 22. 200 l ridged tips, Clear polypropylene and autoclavable, should accurately fit Gilson, Eppendorf and BioHit pipetteman. Required in bulk pack. Quantity: 5,000 tips Made in: USA, Europe, Canada or Japan only

Rack s 10 Boxe s 1 2x 50 1 1000 0 Tube s

10 ac 50 00 s 10 Pack s

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

2. The soft copy of the financial quotation should also be provided on a W/RW CD along with your financial quotation please.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.26/2010 Note:Date of opening 14-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications of consumables for purchase out of PARB Wheat Project 103


Project title: Wheat Crop Improvement for drought tolerance through biotechnology Following materials are required for research project requiring high quality molecular biology and plant tissue culture grade materials. The standardized specifications of the materials meet the product and performance specifications of 36 manufacturers including; 1) 4) 7) 10) 13) 16) 19) 22) 25) 28) 31) 34) Invitrogen Aldrich Roth Roche Promega Ambion Qiagen Riedel-deHaen Axygen Nalgene Greiner CLP 2) 5) 8) 11) 14) 17) 20) 23) 26) 29) 32) 35) Clontech Fermentas Stratagene MRC Bio-Rad MWG/Operon NEB Gene Link Naptune Nunc Nerbe Plus NyxTechnik 3) 6) 9) 12) 15) 18) 21) 24) 27) 30) 33) 36) Sigma Genomed GE Healthcare Phytotechnology Novagen US Biologicals Research Organics Eppendorf Bio Plastic Labnet Corning Oxoid

The quotes for materials from only above mentioned manufacturers may be submitted. The quality of the products and time of delivery per quote is very important and not compromise-able. All the perishable enzymes/materials should be assured for transport in dry ice from the point of origin till those reach the end user. The commodities requiring refrigeration must be transported in ice packs and reach the end user without suffering a heat shock. Humble request to meet these requirements is extended to avoid any later issues as the payment will be released after quality assurance that may take up to 30 days time. Note: Only sole/authorized agents of the above mentioned companies should participate and certificate must be presented at the time of quote. Cat # must be mentioned with the quote for verification. Import documents must be presented at the time of delivery. MB stands for Molecular Biology Grade and DNase/RNase free. Plant Cell Culture Tested (PCCT means the Ultra Purity Grade available to date). Sole/authorized agents must be registered with any Punjab academic / Research institute.

Name of |Item 1. ApaI: (10 units/ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 of lambda DNA in g one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 2. PstI: (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 3. SpeI: (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 4. dNTPs : 100 mM each (not mixed). One set will contain four vials (dATP, dCTP, dGTP and dTTP).4 x 0.25 ml (4 x 25 mol of 100 mM solution). Greater than 99% pure and confirmed by HPLC. Free of human and E.coli DNA. Should be highly stable at neutral pH. The stability should meet a minimum of 100 freeze-thaw cycles. 9095% of dNTPs should remain in triphosphate form even after 7 weeks at room temperature. 85-90% of dNTPs should remain in triphosphate form after 30 cycles of PCR (1 min at 94C; 3 min at 72C). Designed especially for high efficiencies in long range PCR (40 kb), cDNA synthesis, RT-PCR, real-time PCR, standard PCR, and high fidelity PCR. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. 5. Gel Extraction Kit: Required for rapid and efficient purification of DNA fragments from standard gels. Run in TAE or TBE buffer. The kit should be of silica-based membrane technology in the form of spin columns. The kit must have the capacity to purify DNA fragments from 25 bps to 20kb with a recovery rate of upto 95%. The column has the binding capacity of 25 g of DNA and can process upto 1g of agros gel. The process should be completed in 16 minutes time. The eluded DNA should be pure enough to use in applications like ligation, restriction digestion, PCR, Sequencing etc. 6. Genomic DNA purification kit: Required for rapid and efficient purification of high quality genomic DNA

Qty 3000u

3000u

400u

4 sets

2 kits ( 50 preps each)

1 kit (250

from plant tissues. The kit should be of silica based membrane technology in the form of a convenient spin column, eliminating the need for resins, toxic phenol chloroform extractions, or time consuming alcohol precipitation. The procedure should not take more than 20 minutes after cell lises. Isolated DNA could be used for PCR, Southern and enzymatic digestions. The purified genomic DNA should be upto 50kb in size with dominating fragments of 20-30kb. 7. Plasmid Isolation kit: This kit is required for isolation of high quality plasmid DNA from bacterial cultures. The kit must be of silica-based membrane technology in the form of a spin column. The recovery rate should be upto 20ug of high copy plasmid DNA. The isolated plasmid could be directly used for all molecular biology procedures as restriction digestion, PCR, In-vitro transcription, transformation and sequencing. 8. T/A PCR Cloning Kit: PCR Cloning Kit should contain a TA system for direct one-step cloning of PCR products with 3-dA overhangs. PCR products. PCR Cloning Kit should use terminal transferase activity of Taq DNA polymerase and other nonproofreading thermostable DNA polymerases. Enzymes should add a single 3-A overhang to both ends of the PCR product. PCR products should be directly cloned into a linearized cloning vector with single 3-ddT overhangs. The kit must be efficient enough to get 90% of recombinant clones containing the vector with an insert. Recombinant clones must be selected based on blue/white screening. 9. Taq DNA Polymerase: Conc. 10U/ul Thermostable with half life more than 40 min at 95C. Should generate PCR products with 3-dA overhangs and supplied with two buffers 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4 for testing at wide range of magnesium concentrations and decreases unspecific priming. The 25mM MgCl2 should also be supplied. The enzyme should be capable of incorporating modified nucleotides (e.g., biotin-, digoxigenin-, fluorescentlylabeled nucleotides). For routine PCR the amplification of DNA fragments up to 5 kb should be possible. The enzyme should catalyzes 5' to 3' synthesis of DNA with no detectable 3' to 5' exonuclease (proofreading) activity. It should possess negligible or low 5' to 3' exonuclease activity. In addition, Taq DNA Polymerase should exhibit deoxynucleotidyl transferase activity to add extra adenines at the 3'-end of PCR products. One unit of the enzyme should catalyze the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in 30 min at 70C. The error rate of Taq DNA Polymerase should not be more than 2.2x10-5 errors per nt per cycle, and the accuracy of PCR should be 4.5x104. The enzyme must be devoid of E.coli DNA and non-specific amplifications must not be obtained in a template free PCR. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. 10. T4 DNA Ligase: The enzyme should catalyze the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme must be capable of repairing single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids. It should efficiently join DNA fragments with either cohesive or blunt termini, but should not have such activity on single-stranded nucleic acids. The enzyme should remain 100%

reacti ons)

1 kit (250 reacti ons)

2 kits ( 30 reacti ons aech)

25 packs (500 Units each)

1000 Units (5 U/l)

active in restriction enzyme, PCR and RT buffers, supplemented with ATP. One unit (Weiss unit) of the enzyme should catalyze the conversion of 1 nmol of [32PPi] into Norit-adsorbable form in 20 min at 37C. There should be no endo-, exodeoxyribonucleases, phosphatases and ribonucleases activities associated with the enzyme and the quality tests on the enzyme must be there on the product insert. The enzyme should be supplied with 10X T4 DNA Ligase Buffer. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number. 11. cDNA synthesis kit: The First Strand cDNA Synthesis Kit should provide a complete system for efficient synthesis of first strand cDNA from RNA templates. It should be suitable for synthesis of cDNA from 10 to 13 kb. The Reverse Transcriptase included in the kit must be completely devoid of RNase H activity associated with the enzyme to avoid RNA degradation in RNA-DNA hybrids during synthesis of the first strand cDNA. The enzyme should be capable of maintaining activity over a wide temperature range (42-55C), for efficient reverse transcription of RNAs having a high degree of secondary structure. The RNAse inhibitor should be provided in the kit to effectively protect RNA template from degradation. The kit must also include both oligo(dT) and random hexamer primers to support cDNA sunthesis from RNAs with or without poly(A) tail. 12. Real Time PCR Kit: Qty: 200 reaction / kit 2 x 1.25 ml (for 200 react. of 25 l each). Ready-to-use solution optimized for quantitative real-time PCR and two-step real-time RT-PCR with the BioRad iQ machine. The master mix should include Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. The reaction mix should contains SYBR Green I dye supplemented with fluorescein passive reference dye. Only template and primers should be required. The fluorescein included in the master mix should allow for the collection of Dynamic Well Factors with the Bio-Rad iQ5 machine, but should not affect qPCR efficiency. The dUTP should be included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). The nuclease free water should also be a component of the kit for volume adjustments. 13. BamHI: Enzyme; (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 14. EcoRI: Enzyme; (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with 1 kit (100 reacti ons)

2 kits (200 reacti ons /kit)

2x 4000u

2x 5000u

the quote/supply or internet search using catalogue number, which should be clearly provided. 15. HindIII: Enzyme; (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 16. KpnI: (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 17. XbaI: (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 18. Xhol: (10 units /ul) The enzyme should be provided with specific buffers and any other required supplementary materials like BSA, Triton, Tween or DTT solutions. One unit of the restriction enzyme should completely digest 1 g of lambda DNA in one hour. The enzyme should preferably utilize a universal buffer. The prices should be quoted for only required number of units but not bulk packs. The specifications should be easily accessible as a brochure with the quote/supply or internet search using catalogue number, which should be clearly provided. 19. PCR Purification Kit: (250 reactions): Silica membrane based minispin cartridges and associated solutions (gel melting, column wash, DNA elution) capable of 95% recoveries in the range of 100 bp 10 kb DNA fragments and supplied with binding buffer, concentrated wash buffer, elution buffer, purification columns, collection tubes and detailed protocol. The eluted DNA should be pure enough for restriction digestion, DNA sequencing, DNA ligation and labeling.
Manufactured in: USA, Europe or Japan only Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan. 2. The soft copy of the financial quotation should also be provided on a W/RW CD along with your financial quotation please.

2x 5000u

4000u

1500u

2000u

1 kit ( 250 reacti ons /kit)

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.27/2010 Note:Date of opening 15-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications of plasticware for purchase out of PARB Wheat Project 103


Project title: Wheat Crop Improvement for drought tolerance through biotechnology Following materials are required for research project requiring high quality molecular biology and plant tissue culture grade materials. The standardized specifications of the materials meet the product and performance specifications of 36 manufacturers including; 1) 4) 7) 10) 13) 16) 19) 22) 25) 28) 31) 34) Invitrogen Aldrich Roth Roche Promega Ambion Qiagen Riedel-deHaen Axygen Nalgene Greiner CLP 2) 5) 8) 11) 14) 17) 20) 23) 26) 29) 32) 35) Clontech Fermentas Stratagene MRC Bio-Rad MWG/Operon NEB Gene Link Naptune Nunc Nerbe Plus NyxTechnik 3) 6) 9) 12) 15) 18) 21) 24) 27) 30) 33) 36) Sigma Genomed GE Healthcare Phytotechnology Novagen US Biologicals Research Organics Eppendorf Bio Plastic Labnet Corning Oxoid

The quotes for materials from only above mentioned manufacturers may be submitted. The quality of the products and time of delivery per quote is very important and not compromise-able. All the perishable enzymes/materials should be assured for transport in dry ice from the point of origin till those reach the end user. The commodities requiring refrigeration must be transported in ice packs and reach the end user without suffering a heat shock. Humble request to meet these requirements is extended to avoid any later issues as the payment will be released after quality assurance that may take up to 30 days time. Note: Only sole/authorized agents of the above mentioned companies should participate and certificate must be presented at the time of quote. Cat # must be mentioned with the quote for verification. Import documents must be presented at the time of delivery. Sole/authorized agents must be registered with any Punjab academic / Research institute Name of |Item 1. Parafilm Roll (4 inch x 125 ft) Qty 10

It should stretch up to 200 % and cling (seal) around laboratory vessels on even and uneven surfaces. Made of disposable, non-toxic translucent material mainly polyolefins and paraffin waxes. Good gas and water permeability. It should withstand saline, alkaline and inorganic solutions for 48 hours. Withstand temperature from -45 0C to +45 0C 2. Falcon Tubes (50ml) Approximate dimensions: 30 mm O. D.; 115 mm length, sterilized by gamma irradiation and non-pyrogenic, dark color printed graduations and white writing patch, high-density polyethylene flat-top screw cap to allows one hand manipulation, provides a level writing area. Modified polystyrene to improved stress resistance. The top of the cap must have a frosted writing surface for labeling. RNase and DNase free. 3. Ependorf Tubes (1.6ml vol) Clear polypropylene, autoclavable. Unbreakable at 14,000 rpm/16,000 rcf. The tubes should withstand liquid nitrogen. The tubes should not break when spun after liquid nitrogen treatment. The lid/cap should provide enough grip surface for easy opening/closing of tubes at ultra low temperature. Frosted lid surface for writing. Calibrations for volume on the tube surface should be engraved or printed. Required in Bulk Pack. 4. PCR tubes (0.2ml) 0.2 ml thin walled autoclavable and clear polypropylene PCR tubes, optically transparent cap/lid, required in bulk pack. Quantity: 4,000 tubes 5. Petri plates disposable sterile, Size: 92 x 16mm, Disposable and sterile plastic petriplates for plant tissue culture, size 90-100mm (dia) and 15-16mm deep, ; 20 per pack. Quantity 2,000 plates Manufactured in: USA, Europe or Japan only

Rolls

1500 Tubes

50 packs ( 1000/ pk)

15 packs 3000 plates

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No. 28/2010 Note:Date of opening 16-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications for Pipettman (Set of 4) Volume Range: 1. 0.5-10 l 2. 2 -20 l 3. 10 -100 l 4. 100 1000 l Precision volume adjustment Tip Ejection system Made of highly resistant material Lower part autoclave able Warranty: 2-3 years Acceptable accuracy and error at 20-30 oC Specifications standards of Pipettman Accuracy Min. +4.0 Max. +0.5 Min. +4.0 Max. +0.8 Min. +1.6 Max. +0.8 Min. +0.9 Max. +0.6 Permissible Error Min. +0.025 Max. +0.100 Min. +0.10 Max. +0.20 Min. +0.35 Max. +0.80 Min. +0.6 Max. +1.5 (Nos) one one one one

Sr. No. Pipettman II Volume (0.5 l-10 l) Pipetman III Volume (2l-20l) Pipettman I V Volume ( 10l-100l) Pipettman VI Volume ( 100l-1000l) Make: USA, Europe or Japan

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.29/2010 Note:Date of opening 17 -09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications for Ultra Low Temperature Freezer (-80 oC).

Capacity: Temperature Range: Temperature control: Lock and key: Alarm: Voltage: Number of shelves: Outer Door: Inner Doors: Compressor; Gas Refrigerant: Pre-filter:

320 - 500 Liters -40 / -86 oC Microprocessor control, digital LED display and keyboard control panel. Door handles/Latch with lock Multifunction high and low temp battery back-up alarm system 220-240 V; 50 (60) Hz (single phase) 2-3 1 - metallic 3-4 Two air cooled compressors R404A or R407D/R 508, CFC free

Washable pre-filter for protection of condenser fins against dust build-up Body: Stainless Steel interior and powder coated steel finish outer. Warranty: 2-3 years Make: USA, Europe or Japan only

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.30 /2010 Note:Date of opening 20-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications of PCR Machine:

Number of wells:

96 well; Aluminum or alloy block for efficient heat transfer. Tube capacity: 0.2 ml (volume: 10-80 l) Temperature range: 04-99 oC Thermal Accuracy: + 0.2 to 0.3 oC Thermal uniformity: + 0.4 oC well to well within 10 sec of arrival at 90 oC. Thermal Ramping: Average ramp rate 2.5 to 4.0 oC Voltage: 220-240 VAC; 50-60 Hz single phase Heated lid: 110-115 oC Display: Wide Digital LCD display for clear visualization. The minimum display area should be 6-7cm high and 12-14cm wide. Touch Button system but not touch pad Auto-start on power failure Auto-adjusting top lid heating plate Capable of storing 80-400 programs USB port for data transfer Warranty: 2-3 years, licensed and authorized for PCR Manufactured in: USA, Canada, Europe or Japan
Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.31/2010 Note:Date of opening 21-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE:

Specifications for Laminar Flow Cabinet Horizontal Laminar Flow Cabinet to provide class 100 or better environment Locally fabricated 6 Ft Cabinet External Dimension: W (70-80) x H (50-60) x D (25-30) inches Approx. Internal Dimension: W (75-75) x H (20-25) x D (20-25) inches Approx. External Construction: Oven baked epoxy painted steel body Fitted With: HEPA Filter efficiency: 99.99 % @ 0.3 micron Washable synthetic pre-filter dust holding capacity: 90% Fitted with stainless steel working surface Front cover-removable/foldable (steel or fine Perspex) for protection from UV and dust Fluorescent light and UV-germicidal light Inlets for water and Gas (2 sets, one on each side) Two electric receptacles to work with equipment Blower capable to maintain 0.45 m/s face velocity +/- 20 %. Variable speed control Main structure fitted on an iron stand Motor: 1/3 HP single phase, low noise Power requirement: 220v, 50 Hz Accessories: Two additional fluorescent light rods and two UV-germicidal light rods. One set of fine quality pre-filter (dust holder) Two HEPA Filters efficiency: 99.99 % @ 0.3 micron (size: 3 x 2 feet each) One Motor: 1/3 HP single phase, low noise Make: Filter imported and other material local made.
Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.32/2010 Note:Date of opening 22-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specification of the Computer, Printer and accessories:

Processor: Mother Board: RAM: Hard Disk: CD/DVD Rom: LAN Card: Casing: Key Board + Mouse: Monitor: Printer: UPS:

Intel Dual Core 2.93 GHz Intel DG41 RQ Kingston DDRII, 2.0GB Western Digital, 500GB, 7200RPM, SATA Super Driver D-Link Wireless BTX Tower, 400 Watt Power Supply Standard PS2 LCD 19 Laserjet 3 in1 (Printer, Photocopier and Scanner) 600VA, Solid cell batteries, 30 min backup time.

Note: All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.33/2010 Note:Date of opening 23-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications for Pipettman (Set of 4)

Volume Range: 5. 0.5-10 l 6. 2 -20 l 7. 10 -100 l 8. 100 1000 l Precision volume adjustment Tip Ejection system Made of highly resistant material Lower part autoclave able Warranty: 2-3 years Acceptable accuracy and error at 20-30 oC Specifications standards of Pipettman Accuracy Min. +4.0 Max. +0.5 Min. +4.0 Max. +0.8 Min. +1.6 Max. +0.8 Min. +0.9 Max. +0.6

(Nos.) one one one one

Sr. No. Pipettman II Volume (0.5 l-10 l) Pipetman III Volume (2l-20l) Pipettman I V Volume ( 10l-100l) Pipettman VI Volume ( 100l-1000l) Make: USA, Europe or Japan

Permissible Error Min. +0.025 Max. +0.100 Min. +0.10 Max. +0.20 Min. +0.35 Max. +0.80 Min. +0.6 Max. +1.5

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.34/2010 Note:Date of opening 24-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications for Power supply for horizontal gel electrophoresis unites:

Power supply required for horizontal and vertical gel electrophoresis. Output Terminals: Display: Output Voltage: Voltage Accuracy: Output Current: Current Accuracy: Power: Readout Stability: Power cord: On power failure: Timer control: Pause and resume: Input power: Operating Temperature: Humidity: Dimensions: Weight range: Safety features: Warranty: Make: 4-pairs LED screen with 3 digits 10-400 V 2% of reading or 3 volts 4-450 mA 2% of reading or 3 mA, whichever is more 60-100 W. Volts 1 V, Current 1 mA Included Detection and resumption. 0 to 999 minutes Yes 220-240V, 50Hz 0-40C 0-95% 24-26 cm (L) x 20-22 cm (W) x 8-9 cm (H) 1-1.5 kg Overload, overvoltage and short circuit detection and protection 1-2 years USA, Canada, Europe or Japan

1. Mini Horizontal Gel Electrophoresis unite Required for running agaros gels for resolving DNA and RNA samples. Gel Size: Number of Samples: Comb slots: Buffer volume: Electrodes: Included items: Accessories: Mini Combs: Gel casting tray: Make: 2. (W x L) 5-6 x 7-8cm 16-32 samples (when using 2 combs) Two (approx. 1mm well thickness having at least 8 teeth) 300-350 ml at maximum fill mark Made up of Platinum One horizontal gel tank with safety lid having power leads, one gel casting tray (removable) and two combs (approx. 1mm well thickness having at least 8 teeth) Two, Size 1.5mm well thickness, 8 teeth. One tray, for casting 2-3 gels. USA, Canada, Europe or Japan

Midi Horizontal Gel Electrophoresis unite

Required for running agaros gels for resolving DNA and RNA samples. Gel Size: Number of Samples: Comb slots: Buffer volume: Electrodes: Included items: Accessories: Midi Combs: Gel casting tray: Sealing hose: Make: (W x L) 9-10 x 11-12cm 64-80 samples (when using all 4 combs) Four (approx. 1mm well thickness having at least 16 teeth each) 400-450 ml at maximum fill mark Made up of Platinum One horizontal gel tank with safety lid having power leads, one gel casting tray (removable) and two combs (approx. 1mm well thickness having at least 16 teeth) One, Size 1.5mm well thickness, 12 teeth. Two, Size 1.5mm well thickness, 16 teeth. One tray, for casting 2-3 gels. 1 m silicon sealing hose. USA, Canada, Europe or Japan

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.35/2010 Note:Date of opening 27-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications for Single Distillation Still Water Unit

Capacity: Heater: Wattage: Water supply pressure: Distillation conductivity:

4 liters per hour Quartz 2 x 1.5 KW Minimum 5 psi Less than 2.5 s/cm, pH range: 5.5-6.5, Resistance 0.4 MOhmxcm Evaporator and Condenser made of borosilicate glass Protected by safety features and thermostatically shut-off function Electrics connection: 220-240 V; 50 Hz; Single phase Required accessory: Heating Rod: 4 Nos Manufactred: USA, Europe or Japan

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.36/2010 Note:Date of opening 28-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Computer and printer specifications

Processor: Intel Dual Core 2.93 GHz Mother Board: Intel DG41 RQ RAM: Kingston DDRII, 1GB Hard Disk: Western Digital, 320GB, 7200RPM, SATA Driver: Super Driver Wireless LAN Card: D-Link Casing: BTX Tower, 400Watt Supply Key Board + Mouse PS2 Monitor: LCD 19 Printer: Laser Jet 3 in1 (Printer, Photocopier, Scanner) UPS: 600VA Note: All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.37/2010 Note:Date of opening 29-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications for Electrical Conductivity Meter

Description Probe: Size: Battery Power: Conductivity range: Accuracy: Temperature: Display type:

Conductivity probe with 1 meter cable and built in temperature sensor 164mm x 76mm x 45mm 9 V (DC) 0.0 to 199.00 uS/cm = (0.0 to 19.99 mS/cm) = 0.0 to 19.99 dS/m 1% full scale 0-40 oC with built in temperature sensor, adjustable temperature coefficient 0-2.5% per oC. LCD display

Accessories: Dust cover Calibration solutions: Two (500 ml each) Make: Europe, USA and Japan only
Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUIE FOR BIOTECHNOLOGY AND GENETIC NGINEERING P.O.BOX NO.577, JHANG ROAD, FAISALABAD: Tender No.38/2010 Note:Date of opening 30-09-2010 at 1430 hours

ONE STAGE- TWO ENVELOPE PROCEDURE: Specifications for Freezer -20 oC and accessories

Description Temperature Range: Storage capacity: Dimensions: Refrigerant: Voltage: Body: Type: Door: Net weight: Accessories: Voltage Stabilizer Vertical Racks: Make: Local made

-18 to -25 oC 18-25 cubic foot (500-600 Liters) (H x W x D) 1500 to 1700mm x 600 to700mm x 800 to 900mm 134a CFC free 220-240; 50Hz Metallic body Chest Double 80 to 100 kg One (2000Watt) 15 Nos. metallic racks (Stainless steel) Rack size: (H x W x D) 24 x 6 x 6 inches (Column type) for storage of plastic boxes of size: (H x W x D) 5 x 5 x 2 inches

Note:- 1. All the firms who are interested to participate must be registered with any academic or research institute working under Government of Punjab and should not be black listed by any establishment of Pakistan Atomic Energy Commission of Pakistan.

NATIONAL INSTITUTE FOR BIOTECHNOLOGY & GENETIC ENGINEERING PAKISTAN ATOMIC ENERGY COMMISSION, P.O.BOX NO.577, JHANG ROAD F A I S A L A B A D-38000 TENDER NOTICE NO.____________________FOR THE PURCHASE OF _____________________________ TERMS AND CONDITIONS 1. The rates should be quoted on F.O.R. (NIBGE) FAISALABAD destination basis on the enclosed proforma as per accounting/measuring units given therein against each item and in no other accounting /measuring unit, as it entails conversion which is at time difficult in order to determine lowest bidders. The unit and total price should be quoted for all the item. The total price should be summed up for all items as or for each section of the tender in order to show the aggregate price. In case the items are quoted as substitute or an alternate, it should be indicated in the remark column. All clarifications regarding drawings/specifications or otherwise may be sought prior to submitting offers. No excuse will be entertained thereafter that a particular drawing/specifications etc. were not clear to the supplier. Date and time of receipt/opening of bids will be the same as published in the newspapers pertaining to this tender. Tenders can be sent through registered mail or can be delivered in person. Tenders received after the opening date and time, will not be considered but will be returned to the supplier. Validity of rates shall not be less than 90-days from the date of opening. Delivery period shall not be more than 30-days from the date of issue of supply ordered which in exceptional circumstances may be extended with the consent of this Institute. The earnest money at the rate of 2% of the total quoted value in the shape of Deposit-at-Call duly affixed Revenue Stamps according to its value (Cheque, Bank Draft and Demand Draft not acceptable) in the name of Accounts Officer, NIBGE, FAISALABAD only must accompany the offer, failing which the offer is liable to be rejected. Advance payment shall not be possible. Conditional/Telegraphic/Fax offers will not be entertained. Quotation may be sent in closed/sealed Envelope. Supplier on whom the order is placed would be responsible to get the samples approved and deliver the stores within the stipulated delivery period given in the supply order. Stores can be delivered without sample approval but in case of rejection, the Institute will not be responsible for any claim arising thereof. Stores should be despatched with freight pre-paid. Demurrage charges levied due to non-observance of this clause will be deducted from the bill of the supplier for which Institute will not be responsible. All items should suitably be packed with sufficient cushioning material to withstand rough handling during transit. The supplier will be liable to bear any loss due to improper or inadequate packing.

2. 3. 4.

5.

6. 7. 8.

9. 10. 11. 12.

13. 14.

15. Packages should be marked clearly showing consignee as well as the consignor alongwith supply order number an date. 16. Checking and Inspection of stores will be got it done by the supplier or his authorized representative on receipt of stores at this Institute (NIBGE) otherwise deficiency/rejection if any, will have to made good/replaced by the supplier. Part supply will be acceptable, however part payment may not be possible. The supplier on whom the supply order is placed, will provide a warranty/guarantee certificate that stores supplied are brand new and quality conforms to specifications given with supply order. The supplier

17. 18.

including freight charges from to NIBGE to suppliers premises and back will replace any defective parts/stores free of all charges. 19. In case the supplier fails to make the full supply within the stipulated period or abandon during the currency of supply order without reasonable excuse in writing, then the balance of the stores would be arranged through some other agency at the sole Risk & Cost of supplier without any reference in this regard thereof. The judgement of reasonability of excuse rest solely with this Institute. In case the supplier does not supply the stores within the stipulated delivery period, this Institute will be at liberty to confiscate 2% earnest money at the quoted value of the tender even without any reference to the supplier.

20.

20. Quality verification and processing of bills for payment to the supplier will be made on presentation of
following documents:I) II) III) 22. 23. Original TR./RR/Airway bill, if the stores are booked in the name of this Institute (NIBGE). 4-copies of Delivery Challan. 3-copies of Bill/Invoice.

While sending the quotation, envelope should be marked with tender number and date of opening . Calculation mistakes if any found in supply order, be intimated to this office for rectification.

24. This Institute will not be responsible for any loss sustained by the supplier as a result of cancellation of supply order. 25. 26. 27. The Competent Authority reserves the right to accept or reject any or all the quotations. Owner, proprietor or his authorized representative may sign for acceptance of these terms and conditions with a copy of certificate of ownership, proprietorship and authorization. The rates should be in full rupee inclusive of 17% General Sales Tax. If GST not mentioned as Exclusive or Exempted in quotation, the quoted rates shall be considered as Inclusive of 17% GST. Any decimal figure shown with quoted rates shall be considered as deleted/ignored. Writing as GST as per Govt. rules will also be deemed as misleading and shall be considered as inclusive of GST.

The Supply Order will be issued to those Firms/suppliers which are REGISTERED with Sales Tax Department, having their Sales Tax Registration Number on their Tenders/Quotations/Invoices/Bills etc. Note:- The supplied material may be reordered against same price & specifications for three following years subject to consent of supplier and approval of competent authority. Sr. Admin Officer (Procurement) The above mentioned terms and conditions are acceptable to us: Signature:......................................... #........................................................ Name of the bidder with designation: . ......................................................... ..... National Identity Card No. ........ Name of Firm with address................ ................................................ ....................................................... address........................... ....................................................... Telephone Fax E-mail Internet/Web #.............................................. #......................................... site.........................

Telex Grams/Cable

#.