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Code No: RR222305 Set No.

1
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What is hybridization? What is the chemical basis of molecular hybridization?


[16]

2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]

3. Write the differences between the eukaryotic and prokaryotic RNA polymerases and
their mechnism? [16]

4. Describe the following terms: Transcription, translation, promoter. [16]

5. What are mutagens? Classify the radiation, chemical mutagens that are affecting
the organisms. [16]

6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.
[16]

7. Describe the basis for chromosome mapping in the Hf r × F − crosses. [16]

8. What differenciates specialized, generalized and Abortive transduction processes?


[16]

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Code No: RR222305 Set No. 2
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. In what component parts do DNA and RNA differs? [16]

2. What are the differences between continuous and discontinuous DNA replication?
Why does it exist? [16]

3. How does this RNA act as an RNA polymerase? What are the reactions? How
does it differ from a protein RNA polymerase? How is it similar? [16]

4. Discuss the role of the promoter and ribosome sites in regulating gene expression.
[16]

5. Write short notes on

(a) Thymine dimers


(b) Error prone Repair [16]

6. Write a note of Benzer’s fine structure analysis of the rII locus of T4 bacteriophage.
[16]

7. How do you map the chromosome in Hfr bacteria? [16]

8. What are the differences among ans IS elements, a transposon, an intron and a
plasmid. [16]

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Code No: RR222305 Set No. 3
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What were the features of the DNA molecule described by Watson and Crick? Are
these features the same for all DNA molecules? [16]

2. DNA replication is bi-directional. What would the replication bubble look like
(using the same labeling regimen as discussed in class) if replication was unidirec-
tional? [16]

3. What processing events occur with prokaryotic mRNA? [16]

4. Describe the following terms: Transcription, translation, promoter. [16]

5. Write an essay that describes mutations. [16]

6. A point mutation occurs in a particular gene. Describe the types of mutational


events that can restore a functional protein, including intergenic events. Consider
missense, nonsense, and frameshift mutation. [16]

7. Describe the conditions under which genetic recombination may occur in


bacteriophage? [16]

8. Write a note on Virus - mediated bacterial DNA transfer. [16]

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Code No: RR222305 Set No. 4
II B.Tech II Semester Supplimentary Examinations, Aug/Sep 2008
MOLECULAR BIOLOGY
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What were the features of the DNA molecule described by Watson and Crick? Are
these features the same for all DNA molecules? [16]

2. compare conservative, semiconservative and dispersive modes of DNA replication.


[16]

3. What are the general principles underlying all splicing mechanisms? [16]

4. Why would isoleucine be less likely to occur in a beta turn than in a beta strand?
[16]

5. Contrast and compare the mutagenic effects of deaminating agents, alkylating


agents and base analogous. [16]

6. Write a note on molecular structure of eukaryotic gene. [16]

7. Describe the mechanism of transformation process. [16]

8. Outline the steps involved in the replication of a lytic bacteriophage. [16]

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