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Glycolysis
GS/GOGAT cycle
Mito/ chloro
Aminotransferases
Chloroplast
Km 10-80 mM
Km 3-5M
GS1: cytosol, low in leaf high in roots, phloem GS2: chloroplast, mesophyll cells, leaves non-photosynthetic
Subcellular localization: Isoenzymes in cytosol, mitochondria, Chloroplasts and peroxisomes Km values and substrate specificity varies
9/7/2009
Secondary metabolism:
flavonoids alkaloids phenylpropanoids cyanogenic glucosides lignin
9/7/2009
Shikimate pathway
Tree of life
glycolysis
Calvin cycle
branchpoint
Tetrahydrofolate Vitamin A
IAA
Chorismate biosynthesis
4 1 5 2 6 3 7 Bacteria: Separate enzymes catalysing each of the seven steps Fungi: Step 2 to 7 catalyzed by a single polypeptide Plants: Step 3 and 4 Catalyzed by the same enzyme
Aldole condensation
Chorismate biosynthesis
4 1 5 2
Cofactors: NAD, Co2+
Bacteria: Separate enzymes catalysing each of the seven steps Fungi: Step 2 to 7 catalyzed by a single polypeptide
6 x
3 x 7
9/7/2009
NAD or NADP
NADH or NADPH
Shikimate pathway
branchpoint
Tetrahydrofolate Vitamin A
Two isoforms CMI feed back inhibited by phenylalanine and tyrosine, activated by tryptophan CMII not regulated by aromatic amino acids
9/7/2009
Arogenate pathway
Amino acid synthesis proceeds in the chloroplasts Why has this been so difficult to resolve?
Shikimate pathway
EPSP synthase
Marques, p. 447
9/7/2009
C N
Two similar domains each with three domains of folds Only other enzyme with similar architechture is UDP-N-acetylglucosamine enolpyruvyl transferase
Glyphosate inhibition
9/7/2009