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GCSE Additional Science coursework Name: Thomas Tirthdas

How the concentration of hydrogen peroxide effects the rate of reaction in the catalase. I am trying to find out how the concentration of hydrogen peroxide affects the rate of reaction in a catalase. Catalase is an enzyme which is found in all living things. In this investigation I will be using hydrogen peroxide, potato extract (which is found in all potato), 2 test tubes, 40ml beaker, filter paper for absorbing the potato extract, water which will change the percentage of hydrogen peroxide, glass rod and safety goggles to make sure my eyes are always safe and protected. In the pilot task I have 3 set of experiments. Experiment 1 is with a 40ml beaker that contains 40ml of hydrogen peroxide. Experiment 3 is with a test tube that contains 10ml of hydrogen peroxide and experiment 3 is with a test tube that has a flat bottom and contains 10ml of hydrogen peroxide. Furthermore I have done 5 tests for each experiment to make sure the test is fair and reliable. My pilot task results are shown here: Dependant variable (s) Independent 1 2 3 4 5 average variable (ml) 40ml beaker 3.97 7.15 7.35 15.19 7.12 7.206 10ml test 12.73 11.83 8.53 14.10 13.66 13.095 tube 10ml small 12.05 12.15 7.66 8,39 9.03 10.405 test tube The dependant variable on red and on italics is an outlier so therefore it is not included in the average. From these results I found out that the 10ml test tube containing hydrogen peroxide had the slowest average compared to the other test tube. Furthermore the beaker which contains 40ml of hydrogen peroxide has the quickest average time so therefore the beaker with 40ml of hydrogen peroxide is the best experiment to be change. The problem with the methods are that sometimes when we put the filtered paper with the potato extract the potato extract wouldn t rise up and sometimes the potato extract would go up and it would go back down. I change the method to sort out these problems by using a glass rod to push the filter paper down that contains potato extract and I retook the experiment again if the filter paper wouldn t rise up. Methods for investigation I am trying to find out how the concentration of hydrogen peroxide affects the rate of reaction in a catalase. Catalase is an enzyme which is found in all living things. In this investigation I will be using hydrogen peroxide, potato extract (which is found in all potato), 2 test tubes, 40ml beaker, filter paper for absorbing the potato extract, water which will change the percentage of hydrogen peroxide, glass rod and safety goggles to make sure my eyes are always safe and protected.

The variables in this experiment were the time and the amount of potato extract in the filter paper. I controlled these by using a stop watch and also counting for 5 seconds for the filter paper to soak in the potato extract. The variable that I changed was the percentage of hydrogen peroxide. I collected 5 sets of results; I repeated each experiment 5 times. I use the same beaker that contains different concentration of hydrogen peroxide. This was to make sure the test is reliable and fair. Result Table Dependant variable (s) Independent 1 2 variable (ml) 40ml beaker 3.97 7.15 10ml test 12.73 11.83 tube 10ml small 12.05 12.15 test tube

3 7.35 8.53 7.66

4 15.19 14.10 8,39

5 7.12 13.66 9.03

average 7.206 13.095 10.405

Independent 1 2 3 4 5 average variable (%) 20 11.50 13.19 14.72 13.09 12.42 12.98 15 21.75 14.63 19.59 22.81 22.88 21.76 10 25.54 26.65 27.66 19.06 22.23 25.52 5 36.84 55.22 46.18 31.54 49.00 50.13 2.5 44.59 47.50 56.03 48.36 46.68 49.09 The dependant variable on red or on italics is an outlier so therefore it is not included in the average.

Conclusion From my result and my graph I have noticed that there is a pattern. That the more concentrated hydrogen peroxide is the longer it takes for the filter paper to rise up. The scientific explanation for this is because the hydrogen peroxide is concentrated therefore its harder for the catalyse to break up. I have found out that the more concentrated the hydrogen peroxide the longer it takes for the filter paper to go to the top of the beaker.

Evaluation
There were some problems with the experiment. For example the independent variable had outliers, sometimes the filter paper wouldn t sink to the bottom. This might have caused the experiment to be inaccurate and unreliable. To sort out this problem, I could re-do the experiment that contains outliers or not to add my outliers in the average. I think my experiment was not accurate because the experiment contains outlier and the time to measure the filter paper it is not accurate because sometimes you have to press hard on the stop watch to start. I can see that there was some odd result (anomalous result). These are the outlier. They are shown on my table because they are highlighted red or it is on italics. I think these might have happened because there is not enough potato extract in the filter paper or there is not enough hydrogen peroxide for the potato extract to react with the hydrogen peroxide. I am not sure about my conclusion because the experiment is not as reliable as I hope.