MEDICAL BIOCHEMISTRY SYLLABUS 2001

Department of Biochemistry and Molecular Biology University of Arkansas for Medical Sciences College of Medicine

Table of Contents
Page

Lecture Schedule.................................................................................................... i Examination Schedule........................................................................................... iv Course Description................................................................................................ vi Course Texts..........................................................................................................vii Counseling and Office Hours ...............................................................................viii Resolution of Problems.........................................................................................viii Faculty Offices and Phone Numbers.....................................................................viii

MEDICAL BIOCHEMISTRY LECTURE SCHEDULE SPRING SEMESTER 2001

Lecture # 01 02 03 Week 2 04 05 06 07 08 09 10 Week 3 11 12 13 14 15 01/15 01/16 01/17 01/18 01/19 01/19 01/19 Week 4 01/23 1:00 01/25 01/26 Week 5 01/29 01/30 01/31 02/01 02/01 02/02 Week 6 02/05 02/06 02/07 02/07 02/08 HOLIDAY 10:00 Protein Mutations and Disease; lecture 9:00 Nucleotide Structure; Ch. 35 9:00 DNA structure and properties; Ch. 37 8:00 RNA structure and properties; Ch. 37 9:00 DNA organization and replication; Ch. 38 2:00 Review EXAM 1 (8th floor Ed II labs) 9:00 Transcription and RNA processing; Ch. 39 9:00 Control of Transcription-procaryotes; Ch. 41 9:00 9:00 9:00 8:00 9:00 9:00 9:00 10:00 8:00 9:00 9:00 Control of Transcription-eucaryotes; Ch. 41 Protein Synthesis and the Genetic Code; Ch. 40 Synthesis of membrane proteins; Ch. 43 Molecular tools of genetic diagnosis; Ch 42 Clin. Corr. - Acute Myelogenous Leukemia Enzymes; Ch. 8,10 Enzyme Kinetics; Ch. 9 Regulation of Enzyme activity; Ch. 11 Clinical Correlation - Poisoning in a child Enzyme Cascades Blood Clotting; Ch. 59 Clin. Corr. - Severe bleeding in an alcoholic Drake Bannon Bannon Bannon Bannon Drake/Bannon/Straub 01/08 01/09 01/10 01/11 01/11 01/12 01/12 9:00 9:00 9:00 9:00 1:00 9:00 1:00 Biomolecules: Amino Acids and Peptides; Ch. 4,5 Biomolecules: Peptides and Proteins; Ch. 5,6 Higher Order Protein Structures; Ch. 6 Protein Binding Phenomenon; lecture Myoglobin and Hemoglobin; Ch. 7 Clinical Correlation - A Hemoglobinopathy Immunoglobulins as binding proteins; Ch. 59 Drake Drake Drake Drake Drake Straub Drake Date Week 1 Time Topic and Chapter Instructor

01/03 9:00 Introduction, Review of Biomolecules; Ch.1,2 Drake 01/04 10:00 Clinical Correlation - Biochemistry and the Practicing Physician Mock 01/05 9:00 Water, pH and dissociation; Ch. 3 Drake

16 17 18 19 20 21 22 23 24 25 26 27 28

Bannon Bannon Bannon Bannon Bannon Bannon Zent Drake Drake Drake Straub Drake Straub

29

02/09 9:00

Muscle and cytoskeleton; Ch. 58 02/09 2:00 Review

Drake

30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45

Drake/Bannon/Straub Week 7 02/12 9:00 EXAM 2 (8th floor Ed II labs) 02/14 9:00 Overview of metabolism; Ch. 15,16,17 Drake 02/15 9:00 Bioenergetics; Ch. 12 Chambers 02/16 9:00 Glycolysis; Ch. 19 Chambers Week 8 02/19 HOLIDAY 02/20 8:00 Citric Acid Cycle, Pyruvate dehydrog; Ch. 18 Chambers 02/20 9:00 Citric Acid Cycle and anaplerosis; Ch. 18 Chambers 02/21 9:00 Electron transport; Ch. 13,14 Chambers 02/22 9:00 Oxidative phosphorylation; Ch. 14 Chambers 02/23 10:00 Mitochondria,energy, citric acid cycle; Ch.13,14 Chambers Week 9 02/27 9:00 Glycogen metabolism and control; Ch. 20 Chambers 02/28 9:00 Gluconeogenesis; Ch. 21 Chambers 03/01 10:00 Hormonal Regulation: glycolysis/gluconeo; Ch. 51 Chambers 03/02 8:00 Pentose Phosphate pathway; Ch. 22 Chambers 03/02 9:00 Sugar catabolism and biosynthesis; Ch. 22 Drake Week 10 03/05 9:00 Clinical Correlation - Type-II diabetes Wheeler 03/06 9:00 Complex Carbohydrates; Ch. 56 Drake 03/07 10:00 Clinical Correlation - heart failure Straub 03/08 1:00 Review Chambers/Drake/Straub 03/09 9:00 EXAM 3 (8th floor Ed II labs) Week 11 Week 12 03/12 03/16 SPRING BREAK 03/19 03/20 03/21 03/22 03/23 Week 13 03/26 03/26 03/27 03/28 03/29 03/30 03/30 Week 14 9:00 9:00 9:00 9:00 9:00 8:00 9:00 10:00 9:00 9:00 10:00 1:00 Fatty Acid Biosynthesis; Ch. 23 Fatty Acid Oxidation; Ch. 24 Ketone bodies; Ch. 24 Clinical Correlation - hyperlipidemia Eicosanoid metabolism; Ch. 25 Membrane lipid biosynthesis; Ch. 26 Cholesterol and bile acid metabolism; Ch 28 Dean's Distinguished Lecturer - Ketone Bodies Lipid transport and storage; Ch. 27 Lipid transport and storage; Ch. 27 Metabolic Integration; Ch. 29 Water-soluble vitamins; Ch. 52 Lipid-soluble vitamins; Ch 53 Mehta Mehta Mehta Wheeler Mehta Mehta Mehta McGarry Mehta Mehta Mock Mock Mock

46 47 48 49 50 51 52 53 54 55 56 57 58

04/02 9:00

59 60 61 62 Week 15 63 64 65 66 Week 16 67 68 69 70 71 72 Week 17 73 74 75 76 77 Week 18

04/03 8:00 04/04 9:00 04/05 8:00 04/05 9:00 04/09 04/09 04/10 04/11 04/11 04/12 04/16 04/17 04/17 04/18 04/19 04/20 04/23 04/25 04/25 04/26 04/27 9:00 1:00 1:00 9:00 1:00 9:00 9:00 8:00 9:00 8:00 9:00 10:00 9:00 9:00 11:00 8:00 9:00

Clinical Correlation - angina/folate Nutrition; Ch. 54 Digestion and Absorption; Ch 55 Xenobiotic metabolism; Ch 61

Wheeler Mock Mock Raney

Biosynthesis of amino acids; Ch 30 Raney Clinical Correlation - Ataxia and memory loss Straub Nitrogen metabolism; Ch. 31 Raney Amino acid catabolism; Ch.32,33 Raney Review Mehta/Mock/Raney EXAM 4 (9th floor Ed II labs) Specialized amino acids/heme biosynth; Ch. 33,34 Purine metabolism; Ch. 36 Clinical correlation Gout in a heavy drinker Pyrimidine metabolism; Ch. 36 Membrane bilayer properties; Ch 43 Membrane transport; Ch. 43 Membrane receptors; G-proteins; Ch. 44 Clinical correlation - Diarrhea Cancer and Oncogenes; Ch 62 Growth and Differentiation; Ch 62 Biochemical and genetic basis of disease; Ch 63 Review 05/02 9:00 EXAM 5 (8 floor Ed II labs)
th

Raney Raney Straub Raney Haun Haun Haun Raufman Haun Haun Haun Haun/Raney

04/30 1:00 Week 19 05/09 9:00

NBME EXAM (8th floor Ed II labs)

EXAMINATION SCHEDULE
The table below summarizes the schedule and coverage for each examination: Test Exam 1 Exam 2 Exam 3 Exam 4 Exam 5 NBME final Lectures covered 1-15 16-29 30-45 46-66 67-77 1-77 Date 1/23 2/12 3/06 4/12 5/02 5/09 Total Points 150 140 160 210 110 350

Total Course = 1,120

As soon as all students have taken each examination, a key will be made available to you. When any type of question other than machine-graded questions is used on a particular examination, the corresponding key will be posted on the main EdII bulletin board on G level. It is the policy of the Department of Biochemistry and Molecular Biology to administer fair and comprehensive examinations over the subject material taught, and to grade these examinations impartially. The Faculty will consider seriously all honest appeals and will attempt to explain to you why questions are graded as they are. If you feel that a keyed answer is incorrect, a signed written appeal explaining why it is incorrect should be submitted to Dr. Drake directly, either in his office in Barton 5R26, or in his mailbox slot in the main departmental office B405, 4th floor Biomedical Research Building. One appeal of any question will suffice. Frivolous appeals attempting to define, for example, what is is, will not be accepted by Dr. Drake. The appeal will be given to the Lecturer responsible for the question, and he or she will decide whether to accept or reject it. If the appeal is accepted by the Lecturer, all students' scores will automatically be adjusted. If denied, no further recourse is possible. The NBME final examination results are reported as Z-scores and will be converted to a percentage. This percentage will then be applied to the 350 points allocated to the NBME exam. At the end of the Medical Biochemistry course, letter grades will be reported to the Dean's Office on the following basis: > 1,008 points = A > 896 points = B > 840 points = C > 784 points = D < 784 points = F (90.00-100%) (80.00-89.99%) (75.00-79.99%) (70.00-74.99%) (0-69.99%)

Please note that Medical Biochemistry course grading does NOT employ Z-scores, nor will point totals achieved be rounded off. You will know exactly where you stand as the course progresses.

COURSE DESCRIPTION:
The Medical Biochemistry course is designed to provide the novice physician with a fundamental understanding of current concepts of human biochemistry and molecular biology. The course is revised each year to update and improve its content. Hopefully the progression of topics will develop the subject in a fashion useful to the student, especially if he/she feels insecure about chemical and biochemical concepts. The broad topical outline is as follows: 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. The structure and function of proteins in living organisms. The biochemical basis of genotypy and phenotypy and how modern molecular biology provides useful tools for the study of heredity and disease. The role of enzymes in metabolic function The central role of carbohydrate metabolism in energy conservation and utilization by living organisms. Utilization and biosynthesis of lipids by humans and other organisms. Integration of metabolism in the cell, organ and whole organism. Biochemical aspects of human nutrition. The metabolic economy of nitrogen-containing compounds, such as amino acids, purines and pyrimidines. The role of membranes in cell metabolism. The molecular basis of inherited genetic diseases and acquired diseases like cancer

Interspersed throughout the course will be periodic clinical correlation lectures co-ordinated by Dr. Straub, relating to the current topics of the main lecture series. These are meant to illustrate the application of biochemical concepts to a variety of clinical circumstances. Periodic examinations will assess your growing knowledge of the biochemical background necessary for you to become a scientifically informed physician.. No hard-copy, comprehensive syllabus materials will be distributed, however, the exact lecture slides/content will be accessible via your M1 class homepage. Each Lecturer has been asked to submit at least one hard copy of their lecture materials in order to facilitate the canning process (if implemented). A review session in the lecture hall is scheduled before each examination in the lecture hall. The lecturer(s) responsible for portions of that examination will be present to deal with any questions you may have. Attendance at review sessions is not mandatory, but it is encouraged for those needing clarification of any aspect of the lecture series.

BOOKS The required textbook for this course is "Harper's Biochemistry", Twenty-fifth Edition, by Robert K. Murray, Daryl K. Granner, Peter A. Mayes, and Victor W. Rodwell, Appleton and Lange, Norwalk, CT, 2000. ISBN:0-8385-3684-0. The lecturers will use illustrations and assignments from this text whenever possible, but often will supplement this information from other sources. There is also a Board Review Series book entitled "Biochemistry" (ISBN 0-683-05594-1) by Dawn B. Marks that is also recommended but not required. This book contains sample board type questions that may be helpful for selfassessment as you learn the subject of biochemistry. Because individual students have different backgrounds in chemistry and biochemistry, no one textbook will be suitable for all students. The required text has been selected for its coverage, readability and illustrations. In addition, no textbook currently available covers every topic of importance for this course. The individual lecturers will attempt to point out those areas you should understand. Other textbooks you may wish to consult from time to time and that have been used for various courses in our department in the past include: "Basic Medical Biochemistry, A Clinical Approach", by Dawn B. Marks, Allan D. Marks and Colleen M. Smith, Williams and Wilkins, Baltimore, 1996. ISBN 0-683-05595-X "Principles of Biochemistry", 3rd Edition, by Albert L. Lehninger, David L. Nelson and Michael M. Cox, Worth Publishers, New York, 2000. ISBN:1-57259-153-6. "Textbook of Biochemistry with Clinical Correlations", 4th Edition, Thomas M. Devlin, Ed., Wiley-Liss, Inc., New York, 1997. ISBN: 0-471-15451-2. Biochemistry, by Donald Voet and Judith Voet, Wiley-Liss, Inc., New York, 1995 ISBN: 0-471-58651-X. "Principles of Biochemistry", by Geoffrey Zubay, William W. Parson and Dennis E. Vance, William C. Brown Publishers, Dubuque, IA, 1995. ISBN:0-697-14275-2. "Biochemistry", Fourth Edition, by Lubert Stryer, W.H. Freeman and Co., New York, 1995. ISBN:0-7167-2009-4. "Biochemistry", 2nd Edition, by Pamela C. Champe and Richard A. Harvey, J.B. Lippincott Co., Philadelphia, PA, 1997. ISBN: 0-397-51091-8. Useful as a review for the NBME and other parts of the course.

Multimedia Supplements A CD tutorial, UNDERSTAND! Biochemistry (v. 1.1, Mona Group) is available for check out in the Learning Resources Center of the UAMS Library. Most of the topics presented in this course, as well as cell biology topics covered in Microanatomy, can be reviewed using a multimedia approach. Also included on this CD is an extensive series of website links related to each section of biochemistry. We do not officially endorse any of these sites, and they should be used for review purposes only. REMEMBER, the information presented in each lecture and Harpers Biochemistry will be the primary sources for test questions. Another CD available in the LRC is a supplement to the 4th edition of Stryers Biochemistry. There are a few animations on this CD that may be useful to you. Counseling/Office Hours The College of Medicine Policy for counseling students in academic difficulty is described in your Student Handbook. The Department of Biochemistry and Molecular Biology subscribes to this policy. Dr. Drake is available for counseling by appointment whenever you need counseling or assistance with any aspect of Medical Biochemistry, not just when you are having academic difficulty. In addition, Dr. Drake will make every attempt to be available at set office hours from 3:00 4:00 p.m. on Tuesdays and Thursdays. During these times, notification of an intended visit would be appreciated, but not necessary. Resolution of Problems If you encounter a procedural problem or an issue of fairness regarding the Medical Biochemistry course and its administration, bring this matter immediately to the attention of the Course Director, Dr. Drake. If you feel that the issue is not resolved at this level, discuss it further with Dr. Alan Elbein, Chairman of the Department of Biochemistry and Molecular Biology. The Department cannot resolve such issues unless it is aware of them. We are committed to providing a fair and high quality educational experience in the Medical Biochemistry course.

Faculty Offices and Phone Numbers The Departmental Offices are located in the Biomedical Research Center, except where indicated otherwise. Lecturer Dr. Rick Drake Dr. Gary Bannon Dr. Tim Chambers Dr. Randy Haun Dr. Kamal Mehta Dr. Donald Mock Dr. Kevin Raney Dr. David Straub Dr. Alan Elbein, Chairman Biochemistry Office Biochemistry FAX Number Office Location Barton 5R26 B421C B405F B421D B421F Barton 6R08A B421B VA6A1113 B405A B405 Phone Number 686-5419 686-5787 686-5755 686-8594 686-8053 526-4201 686-5244 686-5866 686-5176 686-5196 686-8169