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Biotechnology Final Year



Date of Examination
04 04 2007 07 04 2007 ---------------------

Immunology Animal Biotechnology Practical

50 50 50

Industrial Biotechnology Biotechnology Management & Industrial Relation Practical

50 50

09 04 2007 11 04 2007



Medical Biotechnology Agriculture & Forestry Biotechnology Practical

50 50

13 04 2007 16 04 2007









Unit 1- Introduction of Immunology: Unit 2- Types of Immunity: History of Immunology Scope of Immunology

Natural Immunity Acquired Immunity Active Immunity Passive Immunity Humoral Immunity Cell Mediated Immunity

Unit 3- Cell & Organs of Immune System (Responses & Their functions) Unit 4- Antigen: Unit 5- Antibody: Unit 6- Complement System: Structure Components Properties Functions Antibodies Structure Antibody Types Antibody Properties Functions of Immunoglobulins Production of Antibodies Antigen Types Haptenes Epitopes Factor Influencing Antigenicity

Unit 7- Antigen-Antibody Reaction In-vitro Tests: Precipitation Immunoelectrophoresis Haemagglutination Labeled Antibody o RIA o ELISA o Immuno-Fluroscent Techniques

Unit 8Unit 9-

(A) Hypersensitivity (B) Allergic Reactions (A) Blood Cell Components (B) ABO Blood Grouping Rh Typing

Unit 10- Vaccine: Vaccine & Immunization Active & Passive Immunization Types of Vaccines: o Inactivated o Attenuated o Recombinant Vaccines Peptide Vaccines DNA Vaccines

Animal Biotechnology
Unit 1- Scope of Animal Biotechnology Unit 2- Culture of Media: Simulating natural conditions for Growth of Animal cells Natural Media: Plasma Clot

Unit 3- (A) Primary Culture

(B) Secondary Culture

Biological Fluid Tissue Extract Importance of Serum in Media Chemical Defined Media

Cell Lines & Cloning Disaggregation of Tissue Isolation of Tissue Enzyme Disaggregation & Mechanical Disaggregation

Transformed Animal Cells & Continues Cell Lines

Unit 4- (A) Transfection of Animal Cell Lines

(B) HAT Selection (C) Selectable Markers (D) Transplantation of Cultural Cells

Unit 5- Expression of Cloned Proteins in Animal Cells: Expression Vector Over Production DSP of the Expressed Proteins

Unit 6- Production of Vaccines in Animal cells Unit 7- Monoclonal Antibodies: Unit 8- Growth Factors: Unit 9- Transgenic Animals: Transgenic Animals Techniques Applications Transgenic Mice & Sheep Promoting Proliferation of Animal Cells EGF, FGF, PDGF, IL-1, IL-2 NGF & Erythropoietin Production of Monoclonal Antibodies Application of Monoclonal Antibodies

Industrial Biotechnology
Unit 1(A) Introduction to Industrial Biotechnology (B) Basis Principals of Fermentation Technology Unit 2(A) Screening of Microorganism (B) Isolation of Microorganism (C) Maintenance of Strains Improvement Mutant Selection

Unit 3-

Recombinant DNA Methods

(A) Fermentation Media Natural Media Synthetic Media (B) Sterilization Techniques Heat Radiation Filtration Fermenter Process Aeration Agitation Temperature Regulation Filtration method

Unit 4-

Unit 5-

(A) Fermentation Types of Fermentation Solid State Submerged Fermentation Continues Fermentation (B) Immobilized Enzyme (C) Cell Bioreactors Process Development Shake Flask Fermentation Down Stream Processing (DSP) Distingration of Cells Separation of Products Extraction of Products Concentration of Products Purification of Products

Unit 6-

Unit 7Production of Microbial Products: (Brief account of the following products obtained by Industrial microbiological fermentation) Alcohol Alcoholic Beverage- Beer Organic Acid- Citric Acid Antibiotics- Penicillin Amino Acids- Glutamic Acid Vitamin B12 Brief Account of Steroids

Unit 8-

Enzyme Biotechnology Characteristics of Enzymes Amylases Industrial uses of Enzymes Detergents Leather

Beverage Food Pharmaceutical Bioreactors for Enzyme Production Stirred Tank Membrane reactors Continues flow reactors Yogurt Buttermilk Idly Dosa Cheese Tempeh

Unit 9-

(A) Fermented Foods: (B) Microbial Foods: Single Cell Protein Single Cell Oil

Unit 10- Plant Cell Suspension Culture for the Production of Food Addictives: Saffron Capsaicin Unit 11- Techniques of Mass Culture of Algae: Spirulina Unit 12- (A) Microbial Polysaccharides & Polyesters (B) Production of Xanthan Gum (C) Production of Polyhydroxyalkonoids

Biotechnology Management & Industrial Relation

Unit 1- (A) Biosafety Guidelines & Regulations (B) Patent Related Issues (C) Trading Procedures (D) Copyright (E) IPR Legal & Ethical Issues Concerning Genetic engineering (F Biological Contamination & Bio-Waste Disposal Unit 2(A Bio-Industry Establishment

(B) Examples of Successful Industries in India & Abroad on Biotechnological Processes (C) Recent Trades in the Development of Bio-Industry (D) Bioprocess Costing (E) Project Evaluation Unit 3(A) Biotechnological Institutes in India & World

(B) Their Contribution to Biotechnology (C) Industrial Application of Biotechnology (D) Transfer & Adaptation of Technologies (E) Training of Qualified Personnel (F) International Cooperation in the Field of Biotechnology (G) Scope & Status of Biotechnology Industry in India Unit 4(A) GATT


Medical Biotechnology
Unit 1- Biotechnology in Medicine: Animal & Human health care, Vaccines, Diagnosis including Gene Replacement Therapy, Transplantation of Bone Marrow & Artificial Skin,

Unit 2- Hybridoma Technology: Unit 3- Clinical Immunology: Unit 4- Applied Immunology: Vaccines, T-cell Cloning, Immuno-diagnostics: Immuno-fluorence, Flow Cytometry, RIA, ELISA, Immuno-blotting, Immune-deficiency, AIDS, Transplantation Immunology, Tumor Biology, Introduction, Production of Monoclonal Antibodies, Antibody Engineering using manipulation (Fv, Fab, Fc), Alternative to Hybridoma technology,

Unit 5- Newer Trades:

Genetic Counseling (Antenatal diagnosis, Foetus sexing), Forensic Medicine: Identification of murderers & Rapists etc. Using DNA Fingerprinting technique.

Agriculture & Forestry Biotechnology

Unit 1- Application of Tissue culture in Agriculture: Unit 2- N2 Fixation: N2 Cycle, Sites of N2 Fixation, Self regulatory System, Mechanism, Symbiosis, Factors, Affecting nodules, Development: nod gene, nif gene, nif gene cloning, Transgenic plants, Improvement, Hybrids, Production, Preservation of Synthetic seeds, Production of Disease Resistant Plants, Production of Stress Resistant Plants,

Unit 3- Horticulture: Clonal Propagation of economically impotent plants, Vermicompost, Phosphate Solubilizers, Blue Green Algae, Procedure of Biofertilizers,

Unit 4- Biocontrol of Plant Pathogens, Pests & Weeds: Host Pathogens relation, Predation and Parasitism, Uses of Bt gene, Crop rotation, Microbial pesticides: Bacteria, Virus, Fungi,, Botanical Insecticides: Nicolinoids, Quassincids, Pyrethrins, Rotinoids, Weedicides, Insect as Biocontrol agents,

Unit 5- Cryobiology:

Cryopreservation Methods, Plant Cell Bank, Pollen Bank Uses and Future Prospects of Cryopreservation.