SHORT TERM VOCATIONAL

CERTIFICATE COURSE
(3 Months Duration)

PHLEBOTOMY TECHNICIAN
(SAMPLE COLLECTION)

Prepared by

Neha Malik, M.Sc (Biotechnology), Faculty, Modern

Medical Technologies, Himayathnagar, Hyderabad
&

B. Uma Rani, M. Sc (Nursing),

CF, GJC Shadnagar

Course Coordinator:
R. JYOTHSNA RANI
Lecturer in SIVE,
Commissioner of Intermediate Education, Hyderabad
 Phlebotomy Technician (Sample Collection)

1. Name of the Course : PHLEBOTOMY TECHNICIAN

2. Sector : Para Medical

3. Course Code : PMPT
4. Qualification : S.S.C Pass/ Fail
5. Duration : 3 Months (120 hrs)
Introduction

Phlebotomy Technician program is designed to teach the knowledge in

technical and procedural aspects of basic phlebotomy, including collection of
blood specimens and venipuncture required to become a Phlebotomy Technician.
The Phlebotomy Technician program includes theory and hands-on instruction.
The program will teach students the concepts of Introduction to Phlebotomy &
Infection Control, Legal issues in Healthcare, Introduction to Human Anatomy &
Physiology, Phlebotomy Equipment & Supplies, Phlebotomy Procedures, and
Phlebotomy Fundamental Essentials. This program is designed for leaners who
want to advance their career, or interested in starting a career in the medical field
to become a Phlebotomy Technician.

Objectives of the Course:

 To develop individuals with scientific, technical, innovative and

sophisticated knowledge and skills for medical laboratory
technology related works.
Skills
 To train the student to understand the needs of the management of
the medical labs.
 To train the student to understand the needs and purpose of record
keeping of all the equipments, materials and the patient’s records.
 To train the students to understand the needs of medical lab
equipments sterilization.
Job Opportunities:
 Phlebotomy work is increasing in the medical field, so as the paucity
of Phlebotomist is increasing as there is increase in number of
Hospitals, medical Colleges along with Diagnostics Centres
 Majority of Phlebotomists are employed in the Hospitals or self-
employed (establish own Medical Lab)
 Demand of Phlebotomists in abroad is ever green and can work at
flexible times.
 Phlebotomy Technician in medical laboratories.
Scheme of the Course:

Module Theory On Job Total

Training/Practical
Hours Weitage Hours Weitage Hours Weitage
I 48 40% 72 60% 120 100%

THEORY: 48:00 HRS. MARKS: 30.

S.No. List of Contents No. of periods in Hours.

1. Introduction to Phlebotomy & 6 Hours

Infection Control
2 Legal Issues in Healthcare 5 Hours
3 Introduction to Human Anatomy & 7 Hours
Physiology
4 Medical Terminology 4 Hours
5 Phlebotomy Equipment & Supplies 8 Hours
6 Phlebotomy Procedures I & II 12 Hours
7 Phlebotomy Fundamental Essentials 6 Hours
Total Hours 48 Hours

Unit-I:

Introduction to Phlebotomy and infection control

 Introduction and duties of Phlebotomy technician

 Training, professionalism, License, certification
 Areas of employment
 Laboratory departments
 Occupational Safety and Health Hazard Administration [OSHA]
 Health care safety hazards
 Latex allergy and prevention
 Chain and modes of infection
 Identifying potentially infectious patients like: Contact, Droplet, Airborne
precautions
 Breaking the chain of Infection-Hand hygiene
 Personal Protective Equipment [PPE]-Types & Functions of PPE
 Standard precautions

Unit-II

Legal Issues in Health Care

 Civil law, Tort law

  Negligence / Malpractice
 What is standard of care?
 Basic elements of Negligence
 Types of damages
 Types and sources of laws consent
 Patient’s rights
 Patient self-determination act, advance directives, false imprisonment
 Scope of practice, good Samaritan law, and uniform anatomical gift act
 American with Disabilities Act [ADA]
Unit-III

Introduction to Human Anatomy and Physiology

 Vascular system
 Human blood and Connective tissue
 Formed elements and proportion of Blood
 What is [RBC] / [WBC]?
 Types and Functions of RBC, WBC
 Process of Phagocytosis and Hemostasis
 Blood plasma and serum
 Antibody and Antigen
 Blood transfusion and Blood group
 Blood vessels
 Arterial system: Function and structure - Vasodilation, Vasoconstriction
 Venous system: function and Structure
 Capillaries: Functions
 Veins for phlebotomy
 Body planes
 Directional terms
 Movement terminology
Unit-IV

Medical Terminology

 A-V Alphabet Medical Terminology

Unit-V

Phlebotomy Equipment and Supplies

 Gloves
 Tourniquet
 Alcohol pads
 Gauze
  Bandage
 Needles
 Needle holder
 Sharp container
 Evacuated blood collection tubes &inversion technique
 Blood specimens in Phlebotomy
 Tube additives
 Blood collection color coded tubes
 Order of draw
 Dermal puncture
 Equipment & supplies required for dermal puncture
 Container
 Capillary tubes
 Lancet
 Warming device
 Dermal puncture order of flow
 Centrifuge
 Specimen processing
Unit-VI

Phlebotomy procedure
Competence check list
1) Gloves removal
2) Bleeding time competency
3) Glucose testing competency
4) Capillary tube blood collection procedure
5) Blood smear
6) Vein puncture using multi sample needle [method]-butterfly needle method
Unit-VII

Phlebotomy fundamental essentials

 Vein puncture complications
 Areas of concerns
 Tourniquet test
 How to avoid hemolysis?
 Specimen labeling
 Specimen handling [light, time, temperature]
 Specimen transporting
 Precautions to be considered
 Rejection of specimen
 Test requisition
 Blood collection from pediatric and neonates
 Blood sugar & culture collection
  Therapeutic Drug Monitoring [TDM]
 Urine, stool, sputum collection
 Safety data sheets
 Incident report
On Job Training (Practical)
Time: 72:00 hrs. 70 marks
TOPICS NO. OF PERIODS IN
HOURS.
1.Introduction to Phlebotomy. 12
1.1 Describe the role of a phlebotomy
technician.
1.2 Discuss the areas of employment of a
phlebotomy technician.
1.3 Describe hazards faced by the workers.
1.4 Describe standard precautions.
1.5 Discuss and demonstrate the use of
biohazard container in phlebotomy.
1.6 Discuss and describe bloodborne
pathogen standards.
1.7 Identify special considerations in
phlebotomy.
1.8 Demonstrate techniques of performing
venipuncture.
1.9 Demonstrate techniques of performing
dermal puncture.
1.10 Discuss functions of human body
systems.
1.11 Identify sites of venipuncture.
2. Infection, prevention& precaution. 10
2.1Explain chain of infection.
2.2 Discuss latex allergy and prevention.
2.3 Discuss modes of infection transmission.
2.4 Explain breaking of chain of infection.
2.5 Demonstrate hand hygiene.
2.6 Identify and demonstrate the personal
protective equipment.
2.7 Select correct personal protective
equipment.
2.8 Demonstrate the correct order of
wearing personal protective equipment.
2.9 Discuss post exposure to blood borne
pathogens.
3. Phlebotomy equipments& supplies. 8
3.1Identify phlebotomy equipment used for
performing phlebotomy.
3.2 Identify phlebotomy supplies used for
performing phlebotomy.
3.3 Describe correct specimen transport,
handling, and processing procedures.
3.4 Apply the knowledge learned to fulfill
the job responsibilities of an entry level
phlebotomy technician.

4. Identification &Demonstrate methods. 12

4.1. Identify and demonstrate gloves removal
techniques.
4.2. Identify and demonstrate bleeding time
competency.
4.3. Identify and demonstrate glucose
testing competency.
4.4. Identify and demonstrate capillary tube
blood collection procedure.
4.4. Identify and demonstrate preparing a
blood smear.
4.5. Identify and demonstrate venipuncture
using a multisampling needle (method).
4.6. Identify and demonstrate venipuncture
using a butterfly needle (method).
4.7. Identify and demonstrate venipuncture
using a syringe (method).
5. Phlebotomy Complications, Proper 10
Specimen handling, Transport & Rejection.
5.1. Discuss phlebotomy complication.
5.2. Discuss the areas of concerns on
phlebotomy.
5.3. Discuss and demonstrate tourniquet test.
5.4. Discuss on how to avoid hemolysis.
5.5. Discuss and demonstrate proper
specimen handling techniques.
5.6. Discuss and demonstrate proper
specimen transporting.
 5.7. Discuss the precautions to be
considered. 5.8. Identify and discuss
rejection of specimen.
5.9. Identify phlebotomy test requisition.
6. Sample collections &Testing. 12
6.1. Discuss and demonstrate blood
collection from pediatric and neonates.
6.2. Discuss and demonstrate blood sugar
tests.
6.3. Discuss and demonstrate blood cultures.
6.4. Discuss and demonstrate blood
collection for legal purposes.
6.5. Explain and discuss therapeutic drug
monitoring.
6.6. Discuss and demonstrate urine specimen
collection.
6.7. Discuss and demonstrate stool specimen
collection.
6.8. Discuss and demonstrate sputum
specimen collection.
6.9. Discuss and demonstrate throat swab
specimen collection.
7. Blood donation procedure & Data 8
preparation.
7.1. Discuss and demonstrate blood donation
procedure.
7.2. Discuss and demonstrate safety data
sheets.
7.3. Describe incident report.
7.4. Demonstrate proper documentation
skills.

Qualifications Required for Teaching Faculty:

1.M.D Pathology /Microbiology/Biochemistry/DCP.

2. M.Sc Microbiology / Biochemistry / / Biotechnology/ MLT