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What are
Staphylococci:
Gram positive cocci ( from Greek staphyle, means bunch of grapes ) that occur singly and in pairs, short chains and irregular grape-like clusters.
Gram-positive cocci, nonmotile, facultative anaerobes Cells occur in grapelike clusters because cells division occurs along different planes and the daughter cells remain attached to one another Salt-tolerant: allows them to tolerate the salt present on human skin Tolerant of desiccation: allows survival on environmental surfaces (fomites)
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Staphylocoous appear as
Gram stained smear showing Gram positive cocci with cluster formation indicates presence of Staphylococci
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III.
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Staphylococcus aureus: is the staphylococcus which has the ability to clot plasma, which is coagulase positive. More than 80% of Staphylococcus aureus strains produce beta-lactamases.
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Coagulase test for Identification of Staphylococcus Normal coagulation of plasma Thrombin Fibrinogen Fibrin (soluble ) (insoluble)
*EDTA Rabbit plasma is preferred for the free or tube coagulase test because it contains a large amount of CRF
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Plate 1a: Staphylococcus aureus resistant to penicillin (P0.5) only. Note the annular radius of the zone of inhibition of 9.5 mm around the cefoxitin (FOX 10) disc.
M.R.S.A.
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The programme is created for Undergraduate students in Medical Colleges as a part of Applied Microbiology exercises Email
doctortvrao@gmail.com
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