Using the palm oil wood waste consider one of the greatest method to generate bioethanol from the

wood trunk rather than the normal way that used the palm oil fruit as a biomass generator, it also could save the environment by using the trunk of palm .(instead of burning it and damage our nature. (Wan et al., 2007 Woods consists generally of cellulose that attached in matrix with lignin, hemicelluloses and other substance, this structure effects the enzymatic hydrolysis rate on cellulose. Pretreatment by chemicals, physic-chemical or biological is needed to separate the engagement of these structure to obtain high yield amount of cellulose individually, enzymatic hydrolysis then can be applied to break the cellulose into small segment that will form the glucose and finally with suitable fermentation to the glucose we can obtain the bio fuel or bio ethanol from the palm trunk. (Minoru and .(Isao, 1984 Oil palm trunk tissue analysis usually based on soluble sugar (SS) that mainly form of sucrose followed by glucose and fructose, starch and acid-hydrolysable polysaccharide (PS) that mainly contain of glucose together with xylose and some fructose. Comparison of the total amount of sugar, free sugar and soluble ethanol can take many forms depend on height, site, age of the trunk, etc. In a 10 years old palm trunk we see that relative amount of the biomass is differ in the trunk of palm, petioles and primary roots at two sites that can be called site A and site B. It can be seen that the trunk contain the largest relative amount of the dry biomass by 100 mg/g on both site while it decrease on petioles and primary roots, respectively. It shows that the trunk of palm consider a great resource of generating the dry biomass or bio fuel ethanol and soluble sugars. The methods of measuring and analyze the components of palm wood can be done by sampling the trunk tissue as a first step, following by quantitative and qualitative analysis of carbohydrates to finally obtain a result of the distribution of carbohydrates within the trunk. Comparing the concentration of carbohydrates samples that were taken from the apical of trunk at different ages and from various location from the apex shows that the soluble ethanol sugar which is the main form of the carbohydrates desired was increasing rapidly through ages to reach it highest on eight years old fruiting palm while the starch and Polysaccharides (OP) were highest level at the vegetative palm and reducing the level by increasing of the trunk age of the fruiting palm for the starch and on other hand it was vary for the OP. It also shows the % of free sugars that exist on the trunk were increased by age to

reach it highest level 81.9% at the 8 years old fruiting palm. Comparison of samples that taken from various height up the trunk for an eight years old shows that the extractable sugars and the percentage of the free sugars reach to their highest level as the height increase until reach the apex, from the samples taken it shows that at the apex of 4.60 m the soluble sugars reach to it highest level amount (285 mg/g dry weight) while at height of 1.06 m it was only ( 137 mg/g), same goes to the free sugars that contain about (81.9%) at the apex of the trunk while at 1.06 m the percentage was ( 66.5%). The starch and OP was differ in gradient but it shows that it reach their highest level at half way up to the apex, as we can see that for starch it reach their highest level of ( 77 mg/g) at 2.26 m and the OP highest level is ( 53 mg/g) at 1.86 m height. Another comparison can be based on radial analysis on the same samples, it shows that the highest concentration of soluble ethanol sugars extracted and the free sugars is located in the center of the trunk by (233 mg/g and 76.9%), respectively. Showing also that the concentration of the soluble and free sugars reduce rapidly when reach to the outer cortex, in contrast, it reach to it is highest level for the starch and OP. It noticed that likely the carbohydrate levels in the trunk part will vary with different age and other condition of the palm, it also can be noticed that the soluble ethanol sugars extracted are related to the free sugars percentage in increasing and decreasing and differ from the starch and OP that they are more likely similar to each other. The eight years old palm if we compared it with a 13 years old palm samples we can see that the concentration level of the extractable sugars and free sugars will reduced while the starch and OP will remain similar, it can be conclude that the best extractable level concentration for the soluble sugars can be obtained from the eight years old palm, that contain total carbohydrates almost near to 37 kg which is calculated by adding the amounts of free sugars, starch and other polysaccharides, this value can be equivalent to generate about 20 kg of bunch dry .(matter or 38 kg of fuel facilities branch (FFB). (Henson et al., 1999 Extracting biomass ethanol can also be done from the sap of the old oil palm trunk. The sap of old oil palm trunk found to be contain a large quantity and amount of the glucose content, the concentration level in the inner part of the sap was about 85.2 g/L. The sap of old oil palm trunk is also full of multiple kinds of Vitamins, organic acids and mineral acids. Extracting ethanol and lactic acid ( which is an important and promising materials that can be used in many industrial and bio industrial purpose

such as; bio-plastic and poly-lactate) can be done by fermentation of the glucose in the sap by using brewing yeast strain, Saccharomyces cerevisiae Kyokai no.7 andhomolactic acid bacterium, Lactobacillus lactis ATCC19435, respectively. Existing sugars in the sap palm oil can be increased dramatically due to storage and logging method, it can be show the huge increasing in concentration of the total sugars on the sap from 83 mg/mL to 153 mg/mL after using the storage and logging method. The sugars contained in the sap can be divided into glucose, sucrose, fructose and galactose, that all can be easily fermented using an ordinary industrial yeast strains, that also can be produced the ethanol and lactic acid from the sap without any addition of nutrients at comparable rate and yield to the reference fermentation. .((Akihiko et al., 2010 Experiment for measuring the amount and concentration level of the bio ethanol that we can obtained from the palm oil trunk can be done in several way, collecting three samples of palm oil terena type and 25 years old age cane be used also as a samples 2 extract the desired bio ethanol. After saving the samples at an appropriate temperature and save place we can analyze first the moisture content in our samples before start measuring the concentration level. Determining the total sugars in the sap of the palm oil can be done by used Dubois method that applying phenol and sulfuric acid with a distilled water. After that we can apply the quantitative and qualitative analysis using chromatography method to obtain the results of the total sugars in the sap. The results show that the total sugars was higher in the inner part (A) than the intermediate (B) and outer (c) parts. Comparing the results of the palm oil trunk with sugar cane shows that palm oil trunk producing a higher percentage amount of bio ethanol yield approximately around (8.7-9.4 m3/ha) compared to the sugar cane that produced approximately around (6.5 m3/ha), the moisture contents in the oil palm trunk is slightly less percentage compared to the sugar cane as same as the fermentable sugars .(concentration in the sap. ( Yamada et al., 2010 Extraction of the bio ethanol fuel can be done also using the palm oil fiber. Palm oil fiber is firstly extracted from the empty fruit bunch (EFB) of the oil palm's, it treated then with a retting process that can be a mechanical or chemical retting process. The morphology and properties of the palm oil fiber it nearly similar to wood that containing the prime materials of cellulose, lignin and hemicelluloses. In the palm oil fiber we can see that the percentage range of cellulose is around 43-65, lignin

percentage range is 13-25., hemicelluloses 17 -34 and hololcellulose 68-86 this similar criteria to the woods can allow to use the same steps as firstly pretreatment with an appropriate method like using phenol-formaldehyde to separate the cellulose from the other materials part then apply an enzyme hydrolysis before employing the .(fermentation to get the desired biomass that is the bio ethanol. (Shinoj et al., 2011 As a conclusion, we observed that mainly extracting the bioethanol from palm oil wood or fiber processed likely in the same method and strategy applied, it mainly differ on the aged, height, radial or position of the extracting samples of the trunk palm oil. Palm oil trunk consider a promising resource on the biomass field and an important resource of obtaining and producing the sugars by different way of .(treatment. (Akihiko et al., 2010