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GENE-TRAK ®

For Salmonella DLP Assay

Results

Results

Positive Negative Positive Negative Control Control Sample Sample
Positive
Negative
Positive
Negative
Control
Control
Sample
Sample

Product Specifications

 

Sensitivity:

1-5 CFU/25g

Testing time:

1.5 hours (after 40-48 hour enrichment)

 

Test per kit:

Up to 98

Intended Use

Approvals:

AOAC-RI #961101

GENE-TRAK Salmonella Direct Labeled Probe (DLP) Assay provides for the detection of Salmonella spp. in food.

Materials Required But Not Provided

Available from Neogen*

 

The Test

1. GENE-TRAK photometer set

The DNA hybridization test employs Salmonella-specific DNA probes directly labeled with horseradish peroxidase and a colorimetric detection system for the detection of Salmonella in food samples following broth culture enrichment. A sample is considered negative for the presence of Salmonella if the absorbance value obtained is less than or equal to the established cutoff value for the assay. A sample is considered presump- tively positive for the presence of Salmonella if the absorbance value obtained is greater than the established cutoff value of the assay.

2. Test tube racks

 

3. Dipstick holders

4. Wash basins with lids

5. Lactose broth and/or other pre-enrichment media

6. Tetrathionate broth

 

7. Selenite cystine broth

8. Gram negative broth

9. Pipettes (1 mL and 10 mL)

 

10. Incubator at 36°C ± 1°

11. Incubator at 42°C ± 1°

1. Enrich food samples.

2. Add 0.2 mL of enriched samples (0.1 mL from each culture tube), negative control and positive control into separately labeled 12 X 75 mm glass tubes.

12. Heater block at 37°C and 65°C

13. 12 x 75 mm glass test tubes

14. Minute timer

 

15. Micropipette and tips, 200 – 1000 µL range (optional)

3. Add 0.1 mL lysis reagent to each sample tube. Shake for 5 seconds and incubate at 65°C for 5 minutes.

Not available from Neogen

 

4. Add 0.4 mL of hybridization solution and 0.2 mL of Salmonella probe solution to each tube.

1. Blender or homogenizer

2. Culture bottles for sample pre-enrichment

5. Place dipsticks into sample tubes. Raise and lower dipsticks 10 times to mix, and incubate at 37°C for 1 hour.

3. Culture tubes for 11 mL sample volume

4. Graduated cylinder

6. Wash dipsticks two times for 1 minute each at room temperature.

5. Absorbent paper

 

7. Transfer dipsticks to tubes containing 0.75 mL of substrate-chro- mogen and incubate at room temperature for 20 minutes.

* See Equipment and Accessories, pages 91–95

8. Remove dipsticks and add 0.25 mL stop solution.

9. Read results using a GENE-TRAK photometer.

See package insert for complete instructions

Ordering Information

 

Prod.#

Product description

6702

GENE-TRAK Salmonella DLP Assay - up to 98 tests

Sales & Service: 800/234-5333 (USA/Canada) or 517/372-9200 • Fax: 517/372-2006 • www.neogen.com

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