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GeneQuence™
Listeria

Results

Approvals: AOAC-RI #010403

HPB Canada MFLP-13

Materials Required But Not Provided

Available from Neogen*
Intended Use 1. Microwell reader to read at 450 nm
GeneQuence Listeria provides for the detection of Listeria spp. in food 2. Incubators at 30°C ± 1° and 35°C ± 1°
and environmental samples. 3. 12 x 75 mm glass test tubes and racks
4. 20-200 µL micropipettor and tips
The Test 5. Minute timer
The DNA hybridization test employs Listeria-specific DNA probes 6. Heater block at 37°C ± 1°
which are directly labeled with horseradish peroxidase. A colorimetric 7. Heater block or incubator at 45°C ± 1°
endpoint is then used for the detection of Listeria spp. in food samples 8. 500 mL wash bottle
following enrichment. A sample is considered negative for the presence 9. 10 mL pipette pump
of Listeria if the absorbance value obtained is less than 0.10. A sample 10. 10 mL sterile serological pipettes
is considered presumptively positive for the presence of Listeria if the 11. Multichannel adjustable pipettor
absorbance value obtained is equal to or greater than 0.1. 12. Modified LCA and ceftazidime
13. UVM modified Listeria enrichment broth
Test Procedure 14. Buffered Listeria enrichment broth base (BLEB base)
1. Enrich food or environmental samples. 15. Oxford Listeria agar base
2. Add 0.2 mL of enriched samples and 0.2 mL negative and positive 16. BLEB base supplements
controls to separately labeled 12 x 75 mm glass tubes. 17. Oxford medium antimicrobics (OXA), FDA
3. Add 0.05 mL pretreatment reagent and 0.05 mL lysis reagent to
Not available from Neogen
each sample tube. Shake for 5 seconds, then incubate at 37°C for
1. Blender or homogenizer
5 minutes.
2. Phosphate-buffered saline (PBS)
4. Transfer 0.15 mL of lysed samples to designated microwells.
3. Modified Oxford medium (MOX), FSIS
5. Add 0.125 mL of Listeria probe hybridization solution to each
4. Sodium pyruvate supplement
microwell, mix 5 times with the pipettor, then incubate at 45°C
5. Culture bottles for sample pre-enrichment
for 1 hour.
6. 500 mL graduated cylinder
6. Wash wells 5 times with wash buffer.
7. Sterile cotton swabs and absorbent paper
7. Add 0.15 mL substrate chromogen solution to each microwell and
8. Apparatus for plate washing with vacuum source
incubate at room temperature for 20 minutes.
9. 2 mL disposable graduated pipettes
8. Add 0.05 mL of stop solution to each microwell.
10. Repeater syringe and tips to dispense 50 µL, 100 µL, 125 µL
9. Read absorbance at 450 nm using a plate or strip reader.
and 150 µL volumes (optional)
See package insert for complete instructions 11. Water bath at 37°C (if not using heater block)
12. 12 x 75 mm sterile glass test tubes with caps
Product Specifications 13. Petri plates, 100 x 15 mm
Sensitivity: 1-5 CFU/25g * See Equipment and Accessories, pages 91–95
Testing time: 1 hour, 53 minutes
(after 42-48 hour enrichment)
Ordering Information
Tests per kit: Up to 93
Prod.# Product description
6708 GeneQuence Listeria - up to 93 tests

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