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GENE-TRAK®
Salmonella Assay

Results

Positive Negative Positive Negative

Control Control Sample Sample

Product Specifications
Sensitivity: 1-5 CFU/25g
Testing time: 2 hours (after 40-48 hour enrichment)
Tests per kit: Up to 98
Approvals: AOAC Official Method #990.13
Intended Use
GENE-TRAK Salmonella Assay provides for the detection of Salmonella Materials Required But Not Provided
spp. in food. Available from Neogen*
1. GENE-TRAK 450 nm photometer and standard set
The Test 2. Test tube racks
The DNA hybridization test employs Salmonella-specific DNA probes 3. Dipstick holders
and a colorimetric detection system for the detection of Salmonella 4. Small wash basins
species in food samples following broth culture enrichment. A sample 5. Lids for small wash basins
is considered negative for the presence of Salmonella if the absorbance 6. Dilution chart
value obtained is less than or equal to the established cutoff value for 7. Lactose broth and/or other pre-enrichment media
the assay. A sample is considered presumptively positive for the pres- 8. Tetrathionate broth
ence of Salmonella if the absorbance value obtained is greater than the 9. Selenite Cystine broth
established cutoff value of the assay. 10. Gram Negative broth
11. Incubator at 35°C ± 1°
Test Procedure 12. 12 x 75 mm glass test tubes
1. Enrich food samples. 13. Minute timer
2. Add 0.5 mL of enriched samples (0.25 mL from each culture 14. Pipettor and tips, 100 µL - 1 mL range
tube), negative control and positive control into separately labeled 15. 10 mL sterile serological pipettes
12 x 75 mm glass tubes. 16. 10 mL pipette pump
3. Add 0.1 mL lysis reagent to each sample tube. Shake for 5 seconds 17. Automated tube reader (optional)
and incubate at room temperature for 5 minutes. 18. Heater block at 65°C ± 1°
4. Place rack of tubes in a 65°C water bath. Add 0.2 mL Salmonella
probe solution to each tube. Not available from Neogen
5. Place dipsticks into sample tubes. Raise and lower dipsticks 5 1. Blender or homogenizer
times to mix and incubate at 65°C for 1 hour. 2. Culture bottles for sample pre-enrichment
6. Wash dipsticks for 1 minute at 65°C. 3. Culture tubes for 11 mL sample volume
7. Transfer the dipsticks to tubes containing 0.75 mL of enzyme 4. Graduated cylinder
conjugate and incubate for 20 minutes at room temperature. 5. Absorbent paper
8. Wash dipsticks two times for 1 minute each at room 6. Covered water bath 65°C ± 1° (if not using a heater block)
temperature. * See Equipment and Accessories, pages 91–95
9. Transfer dipsticks to tubes containing 0.75 mL of substrate-
chromogen and incubate for 20 minutes at room temperature.
10. Remove dipsticks and add 0.25 mL stop solution. Read results
using a GENE-TRAK photometer.

Ordering Information
Prod.# Product description
6701 GENE-TRAK Salmonella Assay - up to 98 tests

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