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North Maharashtra University, Jalgaon.

Class:- F.Y.B.Sc.

Subject: Microbiology

With Effect from June, 2009

The board of studies in Life Sciences in its meeting held on 15.04.2009

resolved to accept the revised syllabus for F.Y.B.Sc. (Microbiology) as per guidelines

of Academic Council and with references to UGC model curriculum. The courses

codes and titles for the courses are as given below.

Mb: Microbiology

YSC: Y- Year, S – Semester, C – Course No.



Title of the Course







Mb 111

Elementary Microbiology





Mb 112

Fundamental Methods in Microbiology





Mb 121

Growth & Ultra Structure of Bacteria





Mb 122

Isolation, Characterization & control of Microorganism





Mb 103

Practical Course

1 & 2




North Maharashtra University, Jalgaon

Syllabus for F.Y.B.Sc. Microbiology

(With effect from June 2009)

Mb 111: Elementary Microbiology

Unit I- Types of Microorganism

(12 L)

(General characteristics and Significance of

Following microorganisms)

A) Bacteria

B) Archaebacteria


C) Algae

D) Fungi

E) Protozoa


F) Viruses



Unit II-Scope of Microbiology

(12 L)

a) Microbes in food and Dairy industries

b) Industrial products

c) Genetic engineering and biotechnology

d) Environment

e) Agriculture

f) Bioterrorism

Unit III-History of Microbiology

(16 L)

a) Discovery of Microbial world

b) Spontaneous generation-controversy

c) The golden age of microbiology

i) Fermentation and Pasteurization

ii) The Germ theory of Disease

iii) Vaccination

iv) Chemotherapy- synthesis drugs and Antibiotics

Mb-121: Growth and Ultra structure of Bacteria.

Unit IV – Growth and Reproduction of Bacteria

(18 L)

a) Concept of Growth

b) Reproduction (binary fission, Budding, Fragmentation

Formation of Conidiospores and Sporagiospores)

c) Growth rate and generation time

d) Mathematical expression of growth

e) Growth curve of bacterial population and it’s practical Applications

f) Quantitative measurement of bacterial growth (Total Cell count, viable count- biomass determination, significance of growth measurement)

Unit V-Morphology and Fine Structure of Bacteria

a) Morphology of Bacteria

i) Size and shape

ii) Arrangements

(22 L)

b) Ultra structure of Bacteria

c) Structure, function and chemical composition of the Following

i) Capsule

ii) Flagella

iii) Pili and Fimbriae

iv) Cell Wall (Gram positive &Gram negative)

v) Cell or cytoplasmic membrane

vi) Mesosome

vii) Cytoplasm, Nucleoid (bacterial chromosome) and ribosome’s.

viii) Cytoplasmic inclusion –volutine granules, PHB granules, glycogen, carbohydrates, Magnetosome, Gas vacicals, chlorosome, sulphur granules.

d) Spore and Cyst

i) Endospore and Exospores

ii) Germination and Sporulation of endospore


1. Modi, H.A. Elmentary microbiology Vol .I,Akta Prakashan,Nadiyad.

2. Modi, H.A. Elmentary microbiology Vol.II,Akta Prakashan,Nadiyad.

3. Dubey, R.C.and maheshwari,A. Text Book of Microbiology,S.Chand Publications New Delhi.

4. Tortora,Funke & Case Microbiology-An Introduction ,8 Edn, Pearson Education, Delhi.

5. Pawar and Daginawala, General Microbiology Vol.II. Himalaya Publishing House,Mumbai.

6. Stainer, R.Y.Iingraham,J.L. Wheelis M.L.PainterR.K.(1995) General microbiology, 5 Edition,MacMillan Press Ltd.London.

7. Pelczar,m.j.Chan ECS,Krieg NR(1998)5 Edition,Tata McGraw Hill Publication Co.Ltd.New DELHI.

8. Salle, S.J.(1974).Fundamental Principals of Bacteriology, Tata McGraw Hill Publication Co.Ltd.New DELHI.

9. Frobisher M.Hinsdill,Crabtree and Goodherat(1974) Fundamentals of Microbiology, 9 Edition,WB Saunders Co.USA.

Mb-112: Fundamental Methods in Microbiology

Unit I -: Microscopy

(12 L)

a) Definition

b) Comparison of Bright field and Dark Field Microscope

c) Lenses of cofound microscope

d) Image formation in compound microscope.

e) Numerical aperture and immersion oil

f) Resolving power

g) Magnification

h) Aberration.

Unit II: Biological Staining

(12 L)

a) Concept of stains and Dyes

b) Uses of staining

c) Acidic and basic dyes

d) Mordents.

e) Simple and differential staining (Acid Fast and Gram Staining)

Unit III: Cultivation of Microbes

(16 L)

a) Bacteria

i) Nutritional requirements of bacteria.

ii) Media –Common ingredients

-Construction of culture medium

-Types of Media

iii) Enrichment culture method.

iv) Cultivation of Anaerobic bacteria.

b) Viruses

i) Cultivation of animal viruses (in living animals, Embryonated eggs and cell cultures.)

ii) Cultivation of bacteriophage in laboratory.

Mb-122: Isolation, characterization and Control of Microbes

Unit IV: Pure culture technique and identification of Bacteria

(16 L)

a) Isolation by plating method ( Streak plate, pour plate Spread plate and roll tube method)

b) Isolation in liquid media

c) Two membered cultures

d) Cultural characteristics on solid and in liquid media.

e) Biochemical Characteristics.

Unit V: Control of Microorganisms

(22 L)

a) Fundamentals of Control.

b) Mode of Action of antimicrobial agents.

c) Physical agents – High Temperature, Low temperature, Desiccation, Osmotic PRESSURE, Radiation, Ultraviolet lights,X- rays, Gamma rays,Cathod rays, surface tension and interfacial tension, filtration.

d) Chemical agents : Characteristics of an ideal antimicrobial chemical agent, Definition of Sterilization, Disinfectant, Antiseptic, Sanitizer, Germicidal, Bactericides.

Major groups of chemical antimicrobial agents with practical allocation and mode of



1. Phenol and phenolic compound.

2. Alcohol

3. Halogen

4. Heavy metals and their compounds

5. Dyes

6. Detergents

7. Quaternary ammonium compounds

8. Aldehydes

9. Gaseous sterilization

Practical course: Basic Techniques in Microbiology


Use and care of compound Microscope.


Introduction of laboratory Instruments. Water bath,Autoclave,Hot air oven,pH meter, Weighing balance Specrtophotometer, Centifuge Refrigerator.


Introduction and use of common Laboratory Glass Wares:

i) Test tube, culture tube, screw-capped tubes

ii) Petri dish.

iii) Pipettes

iv) Pasture pipette

v) Erlenmeyer flask

vi) Numeric flask

vii) Glass spreader


Monochrome staining.


Negative staining


Gram staining


Acid fast staining


Hanging drop technique


Preparation of culture medium-(a)nutrient broth and agar,(b)MacConkeys broth and agar


Isolation of bacteria by streak plate technique


Enumarition of bacteria by pour plate technique from soil

sample (serial dilution)


Enumerations of bacteria by serial dilution and spread plate technique from soil sample .

13. Study of commen colony characteristics of (a)E.coli,(b)Staph.aureus.

14. Biochemical test for Identification of bacteria (a) Sugar,GlucoseLactose,Sucrose,Maltose (b)H2s test byT.S.I. and Lead acetate paper technique.

15. IMViC Test.

16. Effect of temperature on growth of bacteria.

17. Effect of pH on growth of bacteria.

18. Effect of heavy metal on growth of bacteria.

19. Demonstration of permanent slides (Anabena, chlorell, mucor, yeast, penicillium, amoeba, paramecium).

20. Growth curve of E.coli.


1. Dubey R.C.and Maheshwari D.K. 2004 Practical Microbilogy, S.Chand and Co. Delhi.

2. Aneja K.R.(1996) Experiments in Microbiology, 3 Edition Wishwa Prakashan,New Delhi.

3. Deshmukh A.M. (1997) 1 Edition, Handbook of Media, Stain and reagents in Microbiology pama publications.

4. Goud R.S. AND Gupta G.D.Practical microbiology, Nirali prakashan, pune.