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Practical 1 Morphological Structure of Cell 3 hours
Practical 2 Micrometer of the cell 3 hours
Practical 3 Cytochemistry and Cytophysiology 3 hours
Practical 4 Cell Mitosis 3 hours
Practical outline:
Practical 1 Morphological Structure of Cell
I. Objective:
⒈The student will master the following skills:
⑴ preparation of cells for examination on the microscope
⑵ staining cells with standard stains
⑶ setting up the microscope for contrast enhancement
⒉ The student will be able to:
⑴ acquaint with the structure of light microscope
⑵ Learn proper handling and become familiar with the use of light microscopes for biological
investigation.
⑶ Master the morphological structure character of cell under the light microscope.
II. Content:
⒈ The usage of optical microscope
⒉ Practise using general optical microscope and observe differient type of tissue cell.
⒊ The main practical slides are provided with:
⑴ human blood smear
⑵ toad blood smear
⑶ mouse small intestine pillar epidermal cell
⑷ skeleton muscle slide
⒋ Manipulation
Make onion epidermal cell temporarily slide
III. Homework:
⒈ Please write down the procedure of the manipulation.
⒉ Make drawing of the onion epidermal cell and label the structure.
II. Content:
⒈ Observation of cellular chemistry composition by optical microscope
1
⑴ glycogen---animal liver smear, PAS reaction
⑵ acid protein---toad blood smear, acid fixed green staining
⑶ alkaline protein---toad blood smear, alkaline acid fixed green staining
⑷ DNA--- animal liver smear, fulgen staining
⑸ lipid---animal liver smear, Sudan Ⅲ staining
⑹ Alkaline phosphoric acid kinase---mouse kidney slide
⒉ Manipulation
Make toad blood smear, using methyl green-pyronin method to show DNA and RNA.
III. Homework:
⒈ Procedure of the manipulation.
⒉ Make drawing of the toad blood smear to show the position of the DNA and RNA.