Give Two Examples (Paper-I)

1. BGA: Anabaena azollae

Nostoc punctiforme
2. Archaebacteria: Methanobacterium thermoautotropicum
Thermoplasma spp
3 .Eubacteria:Thiobacillus thioxidans
Nitrobacter winogradskyi
4. Actinomyces:Streptomyces griseus
Streptomyces erythreus
5. Rickettsia: Rickettsia prowazekii
Rickettsia felis
6. Chlamydia: Chlamydia pneumoniae
Chlamydia psittaci
7. Mycoplasma; Mycoplasma genitalium
Mycoplasma hominis
8. Media for Cultivation of Actinomyces: Actinomyces Isolation Agar
Malt Extract Yeast Extract Agar
9. Media for Cultivation of Rickettsia:Yolk sac of developing Chick Embryo
Mouse Fibroblasts or HeLa Cell Line
10. Thermophilic Archaea metabolizing Sulphur:Sulfolobus acidocaldarius
Sulfolobus thuringenesis
11. Elemental Sulfur Metabolizers:Desulfovibrio desulfuricans
Desulfovibrio desulfomaculum
12. Media for Fungii Cultivation: Corn Meal Agar,PDA
Sabourauds Agar
13. Sulfur Oxidisers:Thiothrix unzii
Thiobacillus thioxidans
14. Gram negative Organisms: Salmonella typhimurium
Escherichia coli
15.Gram Positive Organisms: Bacillus megaterium
Bacillus cereus, polymyxa, anthracis
16.Nitrogrn fixers: Nitrobacter alkalicus
Bradyrhizobium japonicum
Azotobacter vinelandii
17. Diseases Caused by Chlamydia: Pneumonia,
Reiter’s Syndrome and Psittacosis
18. pHB Granules(Lipid) Producers: Bacilllus cereus
Pseudomonas fluorescens
19. Cyanophycin Granules Producers:Anabaena azollae
Nostoc punctiforme
20. Volutin Granules(Metachromatic) Producers: Spirillum volutans
Corynebacterium diptheriae
21. Genera of Yeast: Saccharomyces and Candida
22. Endoflagellar Movement Exhibitors (Spirochetes):Leptospira interrogans
Treponema pallidum
23. Cell Lines for Zoophages: Vero Cell Line from Monkey Kidney Cells
BHK-21 from hamsters
24. Phytophages: Brome Mosiac Virus
Gemini Virus,Cauliflower Mpsiac Virus
25. Viral assays: Hemagglutination Assay
Plaque Assay
26. Monotrichous Flagella: Pseudomonas aeruginosa
Bdellovibrio bacteriovorus
27. Amphitrichous Flagella:Aquaspirillum magnetotacticum
Aquaspirillum serpens
28. Peritrichous Flagella: Proteus vulgaris
Salmonella typhimurium
29. Lophotrichous Flagella: Vibrio fischeri
Pseudomonas fluorescens
30. Viral Capsid Symmetry: Helical-TMV
Icosahedral-Adenovirus
Complex or Binal-T4 phage
31. Basis of Classificatio of algae:-Properties and nature of Pigments
Type,number and morphology of Flagella
Methods of Reproduction
32. Organisms containing Mycolic Acid: Mycobacterium tuberculosis
Mycobacterium diptheriae
33. Amino Acids in Bacterial Cell wall:D-Alanine,D-Glutamine,L-Lysine
34. Components of Murein Layer: GlcNAc and MurNAc
35. Inclusion Bodies: Carboxysomes,Magnetosomes,Gas Vacoules,
Polyglycans,pHB Granules,Proteosomes
36. Pigments produced by Algae: Cyanophycin,phycobillins,Carotenoids
37. Largest Prokayotes:Thiomargarita nambiensis
Epulopiscium fishelsoni
38. Di-amino Acids in Peptidoglycan: D-Lysine and meso-DAP
39. Viral Peplomers: gp 120 , gp41, Hemagglutinin
 40. Cell reconition Molecules in Cell- Cell Interaction: cAMP
Lectins
41. Viral Specific Enzymes: RNA Dependanr RNA Polymerase
Neuraminidase,RT
42. Hemagglutinin Producing Viruses:-Bird Flu Virus
HIV Virus
43. Animal diseases Caused By Prions-Creutzfeldt-Jacob Disease and Kuru
Bovine Spongiform encephalopathy
44. Enxymes in ANIMAL Virus-Neuraminidase and RT
45. Diseases caused by Viriods-Potato Spindle Tuber
Hepatitis D and Exocortis
*Virulence factors by Bacillus anthracis-LPS(Exotoxin) and anthrax Spores

Give Two Examples(Paper-II)

46. Peptide Hormones: Insulin, Oxytocin, Vassopressin, ACTH, LH
47. Fumctions of Vitamin A:Vison(in Vision or Rhodopsin cycle)
Maintainence of epithelial cells of skin,oesophagus
48. Vitamins Associated with ETC: VitK and Riboflavin
49. Sex Hormones : Testosterone and Estrogen
50. Thiamin Deficiency : Anorexia
Wernicke’s Encephalopathy
51. Vitamins involved in Amino Acid Metabolism: Pyridoxine (PLP)
Folic Acid
52. G Protein Mediated Signal transducing Hormone-Adrenaline
Nor-adrenaline

Give Two Examples(Paper-IV)

53. Bioindicators of Sterilization using autoclaves
Bacillus stearothermophilus
Staphylococcus aureus

54. Micro-organisms in Typical Processes:

Wine-Saccharomyces cerevisiae, Candida vini
Penicillin-Penicillium chrysogenum,Aspergillus nidulans
Cheese- Streptococcus cermoris, Streptococcus lactis, Penicillium roquefortii
Yoghurt-Lactobacillus bulgaricus,Streptococcus thermophilus
Bread-Lactobacillus sanfransisco, Saccharomyces exigllus
Vinegar-Acetobacter suboxydans,Acetobacter xylinum
Alcohol-Zymomonas mobilis, Kluveromyces spp.
Beer- S.cerevisiae , S.carlsbergensis
Glutamic Acid- Corynebacterium glutamicum
Micrococcus glutamicus

55. Table wines-Chablis, Sauter, Rhine(All White wines)

Charlet, Rose (All Red Wines)
56. Crude Antifoam Agents-silicone based oils,Olive and linseed oils
57. Antifoam agents or Foam Breakers(Chemical)- Oxazaline, Esters, Sulfonates
58. Amino Acids in Corn Steep Liquor-Glutamic acid and Lysine
59. Inhibitors for Citric Acid Prodn- Mn,Cu,Zn,PO42-
60. Agents for Cell Permeabilization-DMSO,Triton-X,EDTA,Toluene,Propanol
61. Types of Impellors(In Fermenter)-Disc Turbine
Vaned Disc
Open Turbine
62. Flocculating Agents-Tannic Acid, Titanium Tetra chloride,
Borax, Phosphoric Acid
63. Amino Acids in Cane Molasses-Alanine,Glycine,Aspartic Acid
64. Recalcitrant Industrial Waste-Organochlorines
TNT, Pentachlorophenols
Chlorophenoxy alkyl Carboxylic Acids
65. Types of Seal in Fermenter-Simple Stirrer, Mechanical, Stuffing Box
66. Stimulants For citric Acid Production-Ethanol , Methanol, Phytate et al
67. Valves Attached to the Fermenter-Safety, Butterfly, Piston, pressure control
68. Hard Cheese-Cheddar ,Brick and Roquefort cheese
69. Analyzers for detection of O2 in Exit Gases-Deflection analyzer
Thermal analyzer
70. Chelators in Fermentation Medium-Fe ,Zn ,Cu2+
2+ 2+

71. Yeasts for Malo-lactic Fermentation-Schizosaccharomyces pombe

Schizosaccharomyces malidevorans
72. Discolouration in Breads
a. Aspergillus niger-Brown to Black
b. Penicillium notatum-Green color
c. Monilia sitophila-Pink Pigmentation
Instrumentation-PaperII
1.Rotors in Centrifugation-Swing out Bucket Type
Fixed Angle Type
2.Soft β-emitters-Promethium,14C,35S, 3H(Tritium)
3.Support Media for electrophoresis-Agarose gel,
Polyacrylamide, Cellulose Acetate strips
4.Fluors in Scintillators : 2,5-Diphenyloxazole,Butyl-PBD
5.Monochromators in spectroscopy-Grating(Czerny-Turner)
Prism
6.Adsorbents in Chromatography-Silica,Powdered Charcoal,Alumina

Compiled by-
Shreerang R.Bhave
TYBSc Biotechnology’07-08
Thakur college,Kandivali