Fantastic Properties of Nano-bubbles

Kaneo Chiba Masayoshi Takahashi

Burstatthesurface Disappear Continue Shrinking

Goesup rapidly

Ordinarybubble (Macrobubble)

Microbubble

Nanobubble

Nano-bubble production by collapsing microbubble

Waste water treatment
OH Microbubble

Collapse
OH Physical stimulus

Decomposition of organic chemicals Radical generation

Nanobubble production
Microbubble

with salt (electrolytes)

Collapse

Nano-bubble
Physical stimulus

Nono-bubble generator

Microbubble Nano-bubble

Physical stimulus

Analysis of nano-bubble by Dynamic light scattering (DLS)

20 18 16 14 12 10 8 6 4 2 0 76 93 113 138 168 204 249 304 370

Particle size (nm)

The deference of size between MB and NB

Microbubble 10 30m

Nano-bubble < 200nm

The deference of life time between MB and NB

Disappear Continue Shrinking Microbubble Nanobubble

Several minutes

Several months Why ?

Electrode (+)

Light

Electrode ( +)

Water + microbubble Electrophoresis cell Microscope

Movement of microbubbles in electrophoresis cell

Change in potential of microbubble over time

time Ions Accumulation of surrounding ions

-80

Ions Microbubble

with salt (electrolytes)

time

potential mV

-70 -60 -50 -40 -30 -20 0 5 10 15 20 25 30

Diameter m

Stabilization mechanism of nanobubble (assumption)

Nano-bubble
Negatively charged

Aqueous solution

Surrounded by accumulated ions (counter ions) Salting out Prevention of gas dispersion

Nano-bubble generator

Normal ozone water vs. Ozone nano-bubble

Ozone water ; disinfection & sterilizing Food industry, medical Normal method

Ozone Bubbling Nano-bubble

1.5mg/L

0.1mg/L 1 hour

Collapse

1.5mg/L Micro-bubble Nano-bubble

More than 1.0mg/L 100 days 13

How to treat the problem of norovirus

Norovirus
During cultivation

Norovirus symptoms:
vomiting, diarrhea, a low-grade fever, chills, headache, muscle aches

Oyster
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Conventional method

Oyster
Chlorine

Norovirus

Chlorine ( high concentration ) ----- not good for foods

Oyster

Ozone water Ozone = strong oxidation ability Virus inactivation

Norovirus

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Oysters in ozone nanobuble water for 1 day

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Before ozone nanobubble treatment

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After ozone nanobubble treatment

More than 99% of feline calicivirus in the body --- inactivated

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Practical method for inactivation of norovirus by ozone nano-bubble

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Active effect for fishes

Carps : recovering from Carp herpes ( serious disease )

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Oxidation ability of ozone nano-bubble as a form of mist

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more than 80% of original oxidation ability reduction of smell and bacteria from atmospheres
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Oxygen nanobubble

Collapse of oxygen microbubble

About 1% salt Recovery of weakened fishes

Active effect for creatures

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Nano-Bubble Aquarium

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Astonishing effects on plant

Orchid in oxygen NB water for more than 1 month

Still alive

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Initial growth of plants

White radish Broccoli

White radish Broccoli

Tap water

Oxygen nano-bubble water

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Active effect for oysters

Room temperature

-20 for 1 day in oxygen NB

Still alive

After 1 hour 27

Application of nanobubble in medicine Prevention of arteriosclerosis (hardening of blood vessel )

Oxygen nanobubble inhibits mRNA expression induced by TNF in RAOECs ICAM1

VCAM1
Heart attack

GAPDH
control TNF alpha control TNF alpha

Stroke

Culture aorta endothelium of rat Cytokine stimulationTNF alpha

With oxygen nanobubble

Department of Cardiology, Jichi Medical University

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Application of nanobubble in medicine Preservation of living tissue / organ

Vagus nerve of rat : after 1 week Isotonic NaCl solution Oxygen nanobubble

Tokyo Medical and Dental University

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Thank you very much

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