Certicate of Analysis: Lyophilized Microorganism Specication and Performance Upon Release Specications Microorganism Name: Enterococcus faecalis Catalog

Number: 0366 Lot Number: 366-97 Reference Number: ATCC 29212* Purity: Pure Recovery: > 1000 CFUs per Pellet Passage from Reference: 4 Expiration Date: 2012/05 Performance Macrosopic Features: Small to medium, gray/white, translucent, smooth, circular with entire edge Microscopic Features: Gram positive ovoid cells, mostly in pairs or short chains Vitek GP Phenotypic Features Results D-AMYGDALIN + PHOSPHATIDYLINOSITOL PHOSPHOLIPASE C D-XYLOSE ARGININE DIHYDROLASE 1 + BETA-GALACTOSIDASE ALPHA-GLUCOSIDASE + Ala-Phe-Pro-ARYLAMIDASE CYCLODEXTRIN L-Aspartate ARYLAMIDASE + BETA GALACTOPYRANOSIDASE ALPHA-MANNOSIDASE PHOSPHATASE Leucine ARYLAMIDASE L-Proline ARYLAMIDASE BETA GLUCURONIDASE ALPHA-GALACTOSIDASE L-Pyrrolydonyl-ARYLAMIDASE + BETA-GLUCORONIDASE Alanine ARYLAMIDASE + Tyrosine ARYLAMIDASE + D-SORBITOL + UREASE POLYMIXIN B RESISTANCE + D-GALACTOSE + D-RIBOSE + L-LACTATE alkalinization LACTOSE N-ACETYL-D-GLUCOSAMINE + D-MALTOSE + BACITRACIN RESISTANCE + NOVOBIOCIN RESISTANCE + GROWTH IN 6.5% NaCl + D-MANNITOL + D-MANNOSE + METHYL-B-D-GLUCOPYRANOSIDE + PULLULAN D-RAFFINOSE O/129 RESISTANCE (comp.vibrio.) + SALICIN + SACCHAROSE/SUCROSE + D-TREHALOSE + ARGININE DIHYDROLASE 2 + OPTOCHIN RESISTANCE + Medium: SBAP Method: Gram Stain Other Features/ Challenges: Results Catalase (3% Hydrogen Peroxide): negative Bile Esculin Agar: positive Streptomycin (300 mcg - Disk Susceptibility): 14 - 20 mm Gentamicin (120 mcg - Disk Susceptibility): 16 - 23 mm SXT (1.25/23.75 - Disk Susceptibility): >/= 20 mm BHIA w/Vancomycin (6 mcg/ml): Sensitive Additional Information Release Information: Quality Control Technologist: Christine Condon Release Date: 2010/6/25 Disclaimer: The last digit(s) of the lot number appearing on the packing slip is merely a packaging event number. The lot number displayed on this certicate is the actual base lot number.

Brad Goskowicz, President AUTHORIZED SIGNATURE

Note for Vitek: Although the Vitek panel uses many conventional tests, the unique environment of the card, combined with the short incubation period, may produce results that differ from published results obtained by other methods. * The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark and the ATCC catalog marks are trademarks of ATCC. Microbiologics, Inc. is licensed to use these trademarks and to sell products derived from ATCC cultures. 2009 Microbiologics, Inc. All Rights Reserved. 217 Osseo Avenue North Saint Cloud, MN 56303 DOC.286 REVISION 2010.May.20/dt/ml

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