HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIA TITLE : PROCEDURE NO.

BONE MARROW: SAMPLE PREPARATION AND STORAGE HUSM/HEMA-UPT/STM-BM1 VERSION NO. VERSION DATE. 1 24.03.2011

APPROVED BY:

... ASSOC PROF DR ROSLINE HASSAN HEAD OF HAEMATOLOGY DEPARTMENT CONTROLLED COPY NO : 3 REGISTERED HOLDER HAEMATOLOGY LABORATORY RECORD OF REVIEW/AMMENDMENT

DATE

VERSION NO.

DETAIL OF AMMENDMENT

BY

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STANDARD TECHNICAL MANUAL

HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIA TITLE : PROCEDURE NO. BONE MARROW: SAMPLE PREPARATION AND STORAGE HUSM/HEMA-UPT/STM-BM1 VERSION NO. VERSION DATE. 1 24.03.2011

PREPARED BY DESIGNATION CHECKED BY DESIGNATION AUTHORISED BY DESIGNATION

: ANG CHENG YONG : TRAINING OFFICER / SCIENTIFIC OFFICER : DR SHAFINI MOHAMED YUSOFF : HAEMATOLOGIST : ASSOC PROF DR ROSLINE HASSAN : HAEMATOLOGIST/LAB DIRECTOR

1.

OBJECTIVE
To prepare the smears for staining purpose.

2.

METHOD
Bone Marrow spreading

3.

PRINCIPLE The smear should have head and trail

4.

REQUIREMENTS
4.1 EQUIPMENT 4.1.1 4.1.2 4.1.3 4.1.4 4.1.5

Glass slides Spreader Slide holder Gauze Petri dish

4.2 SPECIMEN 4.2.1 Aspirated bone marrow 4.2.2 Imprint 5 PROCEDURE

NO. 5.1
From bone marrow aspiration 5.1.1 5.1.2 5.1.3 Obtain aspirated marrow from the medical personnel immediately and expel it into petri dish Rotate the petri dish to look for the marrow fragment
Gently touches the marrow fragment onto a spreader then transfer it to the glass slide

ACTIVITY

RESPONSIBILITY

MLT/SO MLT/SO MLT/SO

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STANDARD TECHNICAL MANUAL

HAEMATOLOGY DEPARTMENT, HOSPITAL UNIVERSITI SAINS MALAYSIA TITLE : PROCEDURE NO.

5.1.4 5.1.5 5.1.6

BONE MARROW: SAMPLE PREPARATION AND STORAGE HUSM/HEMA-UPT/STM-BM1

VERSION NO. VERSION DATE.

1 24.03.2011
MLT/SO MLT/SO MLT/SO MLT/SO

Drag the fragment and leave a trail of cells behind the spreader Repeat the step 5.1.3 to 5.1.4 to get at least 7 slides for new cases and at least 3 slides for old cases Label the slides with i) Patient R/N and ii) Date of sample collection Allow the slides to dry in the air before stain

5.1.7

5.2

From imprint 5.2.1 5.2.2 5.2.3 5.2.4 Obtain bone biopsy from the medical personnel and placed it in the middle of one glass slide, Placed a second slide on top of the first slides and rolled it forward and backward gently for few times Repeated step 5.2.2 for few time to get at least 2 slides Label the slides with iii) Patient R/N and iv) Date of sample collection Allow the slides to dry in the air before stain

MLT/SO MLT/SO MLT/SO MLT/SO MLT/SO

5.2.5

5.3

For storage 5.3.1 5.3.2 Wrap tightly the additional air-dried unfixed and unstained smear with in aluminum foil Label it with i) Patients name ii) Patients R/N iii) Date of sample collection Storage it at 4 C-10 C for future use purposes
o o

MLT/SO MLT/SO

5.3.3

MLT/SO

REFERENCES
6.1 S. M. Lewis, B. J. Bain & I. Bates. (2006) Dacie and Lewis Practical Haematology, 10 edition. Churchill Livingstone.

th

6.2 S.H.Lee et al. (2008) ICSH guidelines for the standardization of bone marrow specimens and reports. International Journal of Laboratory Hematology 30, 349-364.

End of Document

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