ANNEX II Technical specification for Microstation equipment and a training

UNDP/GEF PROJECT Regional Methyl Bromide, PIMS 3233, ATLAS 46902 Total Sector Methyl Bromide Phase Out in Countries with Economies in Transition Technical specification for Microstation equipment batch 2, BG: The UNDP Regional office in Bratislava wishes to procure equipment and consumables for the identification of soilborne fungi and bacteria, both plant pathogens and potential biological control organisms. The required items and quantities are listed in the attached Annexes A, B and C. All the equipment and materials shall be certified for use in Europe countries / target country. The contractor shall prove that the equipment meets the specifications provided bellow. The contractor shall include in the bid full documentary evidence of this. Specifications for Microstation equipment batch 2, BG: A: MicroStation equipment, databases and consumables Item MicroStation system without computer, 220 VAC Quantity Specification 1 unit Biolog MicroStation System for rapid identification of filamentous fungi, aerobic bacteria and anaerobic bacteria; Including MicroLog 3 Software, electronic user guide, reader, 8 channel pipettor, turbidimeter; Capable of reading all Biolog MicroPlate configurations; Must be fully compatible with the computer system described in Annex D below; 220 volts; power supply suitable for Bulgaria; Warranty of at least one year; 1 unit MicroStation System upgrade 4.2, for system enhancement; Including ownership and registration of databases and new turbidity standards; Must be fully compatible with the computer system described in Annex D below; Biolog FF database for the identification of filamentous fungi, based on the characteristic patterns arising from an isolates reaction to

MicroStation System Release 4.2 upgrade

FF Database for the identification of filamentous

1 database

Item fungi

Quantity

DP Database for the identification of aerobic bacteria and pathogens 1 database

Specification different types of carbon sources; Identification profiles for more than 500 species of fungi from more than 120 genera; including profiles for species of Alternaria, Beauveria, Colletotrichum, Fusarium, Gliocladium, Mucor, Myrothecium, Paecilomyces, Phoma, Rhizopus, Talaromyces, Trichoderma and Verticillium, among many others; Including database software and appropriate turbidity standards; Suitable for use with the Biolog MicroStation listed above; Suitable for operation on the computer system described in Annex D below; Supplied on CD-ROM;

Biolog DP combination database for the identification of aerobic bacteria and pathogens (to be used with GN2 and GP2 MicroPlates); Identification profiles for more than 800 species of aerobic bacteria Including database software and appropriate turbidity standards; Suitable for use with the Biolog MicroStation listed above; Fully suitable for operation on the computer system described in Annex D below; Supplied on CD-ROM; Biolog AN database for the identification of anaerobic bacteria, Database containing identification profiles for more than 350 species of anaerobic bacteria; Including database software and appropriate turbidity standards; Suitable for use with the Biolog MicroStation listed above; Fully suitable for operation on the computer system described in Annex D below Supplied on CD-ROM; FF MicroPlate identification test panels for filamentous fungi; Suitable for use with the Biolog MicroStation components listed in this table;

AN Database for the identification of anaerobic bacteria

1 database

FF MicroPlates for filamentous fungi

100 plates

Page 2

Item GN2 MicroPlates for gram-negative aerobic bacteria

Quantity 30 plates

Specification GN2 MicroPlate identification test panels for gram-negative aerobic bacteria; Suitable for use with the Biolog MicroStation components listed in this table; GP2 MicroPlate identification test panels for gram-positive aerobic bacteria Suitable for use with the Biolog MicroStation components listed in this table; AN MicroPlate identification test panels for anaerobic organisms; Suitable for use with the Biolog MicroStation components listed in this table;

GP2 MicroPlates for gram-positive aerobic bacteria

30 plates

AN MicroPlates for anaerobic bacteria

100 plates

FF-IF filamentous fungi inoculating fluid

100 tubes Sterile inoculating fluid suitable for filamentous fungi; Suitable for use with the Biolog MicroStation components listed in this table; 16 ml per tube (20 x 150 mm tube); 60 tubes Sterile inoculating fluid suitable for gramnegative/gram-positive organisms; Suitable for use with the Biolog MicroStation components listed in this table; 19 ml per tube (20 x 150 mm tube);

GN/GP-IF Gramnegative/gram positive inoculating fluid

AN-IF anaerobic bacteria inoculating fluid

100 tubes Sterile inoculating fluid suitable for anaerobic organisms; Suitable for use with the Biolog MicroStation components listed in this table; 14 ml per tube (20 x 125 mm tube); 500 grams (1 jar) 500 grams (1 jar) 50 droppers Universal growth agar, Biolog BUG; Suitable for use with the Biolog MicroStation components listed in this table; Universal anaerobe growth agar, Biolog BUA; Suitable for use with the Biolog MicroStation components listed in this table; Droppers containing sodium thioglycolate; Suitable for use with the Biolog MicroStation components listed in this table; Transfer pipettes with graduated tip;
Page 3

Biolog universal growth agar

Biolog universal anaerobe growth agar Sodium thioglycolate droppers Transfer pipettes

400

Item

Quantity pipettes (1 box) 9 inch, sterile;

Specification

Ovation pipette tips Reservoirs for use with 8-channel pipette Long Swabs individually wrapped swabs Streakerz pointed wooden streaking sticks

1920 tips Ovation pipette tips; (2 boxes) sterile, racked; Reservoirs for use with 8-channel pipette; 300 reservoirs Sterile; (3 cases) Cotton tipped long swabs; 200 7 inch, sterile, individually wrapped; swabs (2 boxes) 1000 sticks (1 box) Streakerz, pointed wooden streaking sticks; 6 inch, sterile, 20 sticks per bag;

B: Upgrade to Gen III database and related materials Item Gen III Database for aerobic bacteria Quantity Specification 1 Biolog Gen III database for the identification of database gram negative and gram positive aerobic bacteria; Identification profiles for more than 500 species of gram negative bacteria; including species of Burkholderia, Erwinia, Pseudomonas and Xanthomonas, among many others; Identification profiles for more than 300 species of gram positive bacteria, including species of Bacillus, Clavibacter and Paenibacillus, among many others; Including database software and appropriate turbidity standards; Suitable for use with the Biolog MicroStation listed in Annex A; Fully suitable for the computer system described in Annex D below; Supplied on CD-ROM; 60 plates Gen III MicroPlate identification test panels for gram positive and gram negative aerobic bacteria; Suitable for use with the Biolog MicroStation components listed in Annex A; IF-A sterile inoculating fluid suitable for gramPage 4

Gen III GN/GP MicroPlates for aerobic bacteria

IF-A inoculating

60 tubes

fluid for Gen III MicroPlates (aerobic bacteria) IF-B inoculation fluid for Gen III MicroPlates IF-C inoculation fluid for Gen III MicroPlates Inoculatorz applicator swabs 20 tubes

negative/gram-positive organisms; Suitable for use with Gen III MicroPlates; IF-B sterile inoculating fluid suitable for gramnegative/gram-positive organisms; Suitable for use with Gen III MicroPlates; IF-C sterile inoculating fluid suitable for gramnegative/gram-positive organisms; Suitable for use with Gen III MicroPlates; Inoculatorz cotton tipped swabs for Gen III MicroPlates; individually wrapped, sterile;

20 tubes

100 swabs (1 box)

C: On-site training for MicroStation system Item Specification Provide on-site training in the use of the MicroStation equipment and related consumables; Training to be carried out by a trainer who has good working knowledge of the MicroStation system; Training to be carried out in Plovdiv, Bulgaria. Suppliers are requested to submit a list of topics that will be covered in the training sessions, and information that demonstrates the trainer has relevant qualifications and experience to carry out training for Biolog MicroStation systems

Provision of training for the use of MicroStation system

Information on computer compatibility All of the software and equipment listed in Annex A and B must be entirely suitable for operation with the following computer systems: Pentium PC with 512 cache, 233 mHz minimum 128 MB RAM minimum 250 MB free hard disk space High-density floppy drive CD-ROM drive: 12-24x speed Microsoft-compatible two-button mouse Monitor: 800 x 600 pixel resolution: 6 bit, 256 colour Microsoft Windows NT, Windows 2000 or Windows XP
Page 5

The bidder should also provide: -

Certificate of quality standard Service guarantied in Bulgaria Guarantee at least 2 years drawings and photos of the equipment Transport to Bulgaria to the indicated delivery place

Delivery period 6 weeks after signing the contract Delivery place: Agricultural University of Plovdiv 12, Mendeleev Blvd. 4000 Plovdiv Bulgaria

Page 6