Mouse Anti-Cytokeratin 18 M30 CytoDEATH Monoclonal Antibody, Unconjugated, Clone M30 from Roche Applied Science

Product Mouse Anti-Cytokeratin 18 M30 CytoDEATH Monoclonal Antibody, Unconjugated, Clone M30

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Roche Applied Science Get Quote 12140322001 50 tests Unconjugated Request Info Request Info Monoclonal Antibody Cytokeratin 18 Clone M30 Mouse Roche Applied Science: Product Info

Description

Assay time: 2 hours for immunofluorescence on cells, 3.5 hours for staining of tissues (excluding dewaxing) Sample material: Adherent cells, tissue samples (routinely fixed and paraffin-embedded tissue sections, cryostat sections) Specificity: The M30 CytoDEATH antibody binds to a caspasecleaved, formalin-resistant

M30 CytoDEATH

Mouse monoclonal antibody (Clone M30) for the detection of apoptosis in epithelial cells (caspase cleavage product of cytokeratin 18)Formalin grade Cat.No. Pack Size Qty Price

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12140322001 50 tests 12140349001 250 tests

223.23 533.81

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Application
Use the M30 CytoDEATH antibody for the determination of early apoptosis in cells and tissue sections. A specific epitope of cytokeratin 18, which is presented after cleavage by caspases, is detected.

Benefits

Convenient: Easy-to-use standard protocol Clear results: Apoptotic cells are most easily distinguishable from normal cells Early detection of apoptosis: Detects caspase-cleaved Cytokeratin 18; caspase activity is one of the earliest apoptosis markers Useful for paraffin-embedded tissue: Useful for routinely fixed tissue samples; retrograde studies are possible

Product Description
Assay time: 2 hours for immunofluorescence on cells, 3.5 hours for staining of tissues (excluding dewaxing) Sample material: Adherent cells, tissue samples (routinely fixed and paraffin-embedded tissue sections, cryostat sections)

Specificity: The M30 CytoDEATH antibody binds to a caspasecleaved, formalin-resistant epitope of the cytokeratin 18 (CK 18) cytoskeletal protein. The immunoreactivity of the antibody is confined to the cytoplasm of apoptotic cells.

Background Information
During apoptosis, vital intracellular proteins are cleaved. The proteases that mediate this process are called caspases (Cysteinyl-aspartic acid proteases). Caspases are expressed as zymogenes, which are activated by different apoptosis inducers. Once activated, a single caspase activates a cascade of caspases. Until recently, cytokeratins, in particular cytokeratin 18, were not known to be affected by early events of apoptosis. Recently, it has been shown that the M30 antibody recognizes a specific caspase-cleavage site within cytokeratin 18, which is not detectable in native CK18 of normal cells. Consequently, the M30 CytoDEATH antibody is a unique tool for the easy and reliable determination of very early apoptotic events in single cells and tissue sections.

Contents
Mouse monoclonal antibody (clone M30), lyophilized, stabilized, formalin grade.

Typical Experiment
Short protocol For formalin-embedded tissue:

Dewax formalin-fixed, paraffin-embedded tissue sections. Retrieve antigen by heating in citric-acid buffer. Add M30 antibody. Add Anti-mouse-biotin. Add Streptavidin-POD. Add substrate solution (DAB or AEC). Counterstain with Harris hematoxylin. Analyze under a light microscope.

For immunofluorescence on cells:

Fix cells. Add M30 antibody. Add Anti-mouse-Ig-fluorescein. Analyze under a fluorescence microscope.

Figure 2: Human colon tissue (research sample) demonstrating positive staining for caspase-cleaved cytokeratin 18. Detection of apoptosis with M30 CytoDEATH, anti-mouse-biotin, streptavidin-HRP, and AEC (substrate). Counterstained with hematoxylin. M30 staining (brown) appears in the cytoplasm of the cell. This assay works well in combination with TUNEL reactions.