LEMBAR KERJA PRAKTIKUM PEMERIKSAAN JAMUR TUJUAN Mengamati gambaran mikroskopik jamur dan membedakannya dari bakteri Mengamati

gambaran biakan jamur pada agar Sabouraud Memahami dan mengerjakan pembuatan preparat dan pengecatan jamur dengan cat Gram dan LPCB, dari spesimen jamur yang telah disediakan. DASAR TEORI Tulislah dasar teori berdasarkan pustaka sebanyak 150-200 kata.

ALAT Mikroskop Gelas obyek Deck glass Ose bulat Kertas label BAHAN Spesimen untuk pemeriksaan jamur Contoh preparat mikroskopik Candida sp Contoh biakan jamur pada agar Sabouraud : C. albicans, Rhizopus sp, Trichophyton sp, Trichophyton rubrum, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger Cat Gram A, B, C dan D Cat LPCB KOH 10-20 %
Laboratorium Mikrobiologi FK UII 1

SKEMA KERJA Pemeriksaan Jamur 0100090000032a0200000200a20100000000a201000026060f003a03574d4643010000 0000000100c9a40000000001000000180300000000000018030000010000006c000000 00000000000000001f000000420000000000000000000000744500006c69000020454d 46000001001803000012000000020000000000000000000000000000004c0b00001d10 0000cc000000230100000000000000000000000000000f1d0300e2700400160000000c 000000180000000a0000001000000000000000000000000900000010000000d8090000 f10e0000250000000c0000000e000080250000000c0000000e000080120000000c00000 001000000520000007001000001000000c9ffffff00000000000000000000000090010000 0000000004400022430061006c00690062007200690000000000000000000000000000 0000000000000000000000000000000000000000000000000000000000000000000000 00000000420048a7420010000000acaa42002ca84200824cf06aacaa4200a4a74200100 0000014a9420090aa4200544cf06aacaa4200a4a742002000000049641f6da4a74200ac aa420020000000fffffffffc028c00d0641f6dffffffffffff0180ffff0180efff0180ffffffff0000000 000080000000800004300000001000000000000006801000025000000372e900100000 20f0502020204030204ef0200a07b20004000000000000000009f000000000000004300 61006c0069006200720000000000000000006ca84200dee31e6de88df86dccab4200d8 a742009c38176d070000000100000014a8420014a84200e878156d070000003ca84200 fc028c006476000800000000250000000c00000001000000250000000c0000000100000 0250000000c00000001000000180000000c00000000000002540000005400000000000 000000000001f0000004200000001000000e4b9e140d6bee1400000000034000000010 000004c000000040000000000000000000000d8090000f10e00005000000020001f0020 00000046000000280000001c0000004744494302000000ffffffffffffffffd9090000f20e000 0000000004600000014000000080000004744494303000000250000000c0000000e000 080250000000c0000000e0000800e00000014000000000000001000000014000000040 0000003010800050000000b0200000000050000000c02fe02f801040000002e0118001c 000000fb020300010000000000bc02000000000102022253797374656d00004d532053 68656c6c20446c67000000000000000000000000040000002d010000040000002d0100 0004000000020101001c000000fb02f5ff0000000000009001000000000440002243616c 6962726900000000000000000000000000000000000000000000000000040000002d01 0100040000002d010100040000002d010100050000000902000000020d000000320a0a 0000000100040000000000f701fd0220000600040000002d010000040000002d0100000 30000000000

* Pengecatan LPCB 0100090000032a0200000200a20100000000a201000026060f003a03574d4643010000 0000000100c9a40000000001000000180300000000000018030000010000006c000000 00000000000000001f000000420000000000000000000000744500006c69000020454d 46000001001803000012000000020000000000000000000000000000004c0b00001d10 0000cc000000230100000000000000000000000000000f1d0300e2700400160000000c 000000180000000a0000001000000000000000000000000900000010000000d8090000 f10e0000250000000c0000000e000080250000000c0000000e000080120000000c00000 001000000520000007001000001000000c9ffffff00000000000000000000000090010000 0000000004400022430061006c00690062007200690000000000000000000000000000 0000000000000000000000000000000000000000000000000000000000000000000000 00000000420048a7420010000000acaa42002ca84200824cf06aacaa4200a4a74200100 0000014a9420090aa4200544cf06aacaa4200a4a742002000000049641f6da4a74200ac aa420020000000fffffffffc028c00d0641f6dffffffffffff0180ffff0180efff0180ffffffff0000000 000080000000800004300000001000000000000006801000025000000372e900100000 20f0502020204030204ef0200a07b20004000000000000000009f000000000000004300 61006c0069006200720000000000000000006ca84200dee31e6de88df86dccab4200d8 a742009c38176d070000000100000014a8420014a84200e878156d070000003ca84200 fc028c006476000800000000250000000c00000001000000250000000c0000000100000 0250000000c00000001000000180000000c00000000000002540000005400000000000 000000000001f0000004200000001000000e4b9e140d6bee1400000000034000000010 000004c000000040000000000000000000000d8090000f10e00005000000020001f0020 00000046000000280000001c0000004744494302000000ffffffffffffffffd9090000f20e000 0000000004600000014000000080000004744494303000000250000000c0000000e000 080250000000c0000000e0000800e00000014000000000000001000000014000000040 0000003010800050000000b0200000000050000000c02fe02f801040000002e0118001c 000000fb020300010000000000bc02000000000102022253797374656d00004d532053 68656c6c20446c67000000000000000000000000040000002d010000040000002d0100 0004000000020101001c000000fb02f5ff0000000000009001000000000440002243616c 6962726900000000000000000000000000000000000000000000000000040000002d01 0100040000002d010100040000002d010100050000000902000000020d000000320a0a 0000000100040000000000f701fd0220000600040000002d010000040000002d0100000 30000000000

Membuat mikrokultur (slide culture)

Laboratorium Mikrobiologi FK UII

0100090000032a0200000200a20100000000a201000026060f003a03574d4643010000 0000000100c9a40000000001000000180300000000000018030000010000006c000000 00000000000000001f000000420000000000000000000000744500006c69000020454d 46000001001803000012000000020000000000000000000000000000004c0b00001d10 0000cc000000230100000000000000000000000000000f1d0300e2700400160000000c 000000180000000a0000001000000000000000000000000900000010000000d8090000 f10e0000250000000c0000000e000080250000000c0000000e000080120000000c00000 001000000520000007001000001000000c9ffffff00000000000000000000000090010000 0000000004400022430061006c00690062007200690000000000000000000000000000 0000000000000000000000000000000000000000000000000000000000000000000000 00000000420048a7420010000000acaa42002ca84200824cf06aacaa4200a4a74200100 0000014a9420090aa4200544cf06aacaa4200a4a742002000000049641f6da4a74200ac aa420020000000fffffffffc028c00d0641f6dffffffffffff0180ffff0180efff0180ffffffff0000000 000080000000800004300000001000000000000006801000025000000372e900100000 20f0502020204030204ef0200a07b20004000000000000000009f000000000000004300 61006c0069006200720000000000000000006ca84200dee31e6de88df86dccab4200d8 a742009c38176d070000000100000014a8420014a84200e878156d070000003ca84200 fc028c006476000800000000250000000c00000001000000250000000c0000000100000 0250000000c00000001000000180000000c00000000000002540000005400000000000 000000000001f0000004200000001000000e4b9e140d6bee1400000000034000000010 000004c000000040000000000000000000000d8090000f10e00005000000020001f0020 00000046000000280000001c0000004744494302000000ffffffffffffffffd9090000f20e000 0000000004600000014000000080000004744494303000000250000000c0000000e000 080250000000c0000000e0000800e00000014000000000000001000000014000000040 0000003010800050000000b0200000000050000000c02fe02f801040000002e0118001c 000000fb020300010000000000bc02000000000102022253797374656d00004d532053 68656c6c20446c67000000000000000000000000040000002d010000040000002d0100 0004000000020101001c000000fb02f5ff0000000000009001000000000440002243616c 6962726900000000000000000000000000000000000000000000000000040000002d01 0100040000002d010100040000002d010100050000000902000000020d000000320a0a 0000000100040000000000f701fd0220000600040000002d010000040000002d0100000 30000000000 Tugas praktikan : Membuat preparat dari spesimen jamur yang tersedia (2 spesimen) Melakukan pengecatan preparat dengan cat Gram dan LPCB Menggambar hasil contoh preparat mikroskopik Candida sp Menggambar hasil contoh biakan jamur pada agar Sabouroud: C. Albicans, Rhizopus sp, Trichophyton sp, Trichophyton rubrum, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger HASIL

Laboratorium Mikrobiologi FK UII

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Laboratorium Mikrobiologi FK UII