Arundhati Ghosh, Ph.D.

Columbia, MD 21044 443-827-7122 (Cell) aghosh10@hotmail.com PROFILE Versatile biomedical scientist with extensive research experience in cancer biology and diverse training and research expertise in full-scale project lifecycles in microbiology, molecular biology biochemistry, and cell-signaling. Experienced and efficient in planning, coordinating and executing multiple projects in parallel , mentoring/training junior colleagues, maintaining lab inventory/equipment.

Efficient in presenting and writing scientific results, executing collaborations.

Dynamic, adaptable, goal-orientated, team player with excellent interpersonal and communication skills, with multicultural competencies, spanning professional/educational experience across three continents. STATUS Legal Permanent Resident of the USA. EDUCATION Ph.D. (1997-2002): Environmental Microbiology, Kings College, London, University of London (UK). B.Sc. (1994-1997): Biotechnology with a First Class Honours degree, Kings College, London (UK). PROFESSIONAL EXPERIENCE University of Maryland, Baltimore Postdoctoral fellow, Greenbaum Cancer Center, Dec 2009-Present. Studying the mechanisms by which the levels of ErbB2 protein are affected by the EBP1 (ErbB3-binding protein-1) and PAK1 levels in human breast cancer cells. Understanding this complex relationship and interplay may lead to better breast cancer therapies. Second project involved elucidating the affects of EBP1 protein binding to the ErbB2 promoter region after ligand stimulation and its affect on gene regulation. Postdoctoral fellow, Center for Vascular & Inflammatory Diseases, Jan 2007-Oct 2009 Investigated the effect of juxtacrine signalling on various signalling pathways in brain cancer cells. This work resulted in a journal publication in the Journal of Biological Chemistry in 2010. Studied the mechanism by which the interaction between the ligand, TWEAK, and its receptor, Fn14, results in invasion, metastasis and angiogenesis in brain cancers. Postdoctoral fellow, Mucosal Biology Research Center, Aug 2004-Jan 2007 Studied the effect of heat shock factor-1 (HSF-1) on cytokine gene expression under fever conditions. This work resulted in a journal publication in the American Journal of Physiology - Cell Physiology in 2010. Second project investigated which transcription factors were recruited to the 5UTR promoter site of TNFalpha during fever conditions. Understanding this process would help us better treat patients during sepsis conditions in the clinic using piperidine-cleaved DNA footprinting and ChIP. Postdoctoral fellow, Department of Pharmaceutical Sciences, April 2002-May 2004 Project involved the cloning, purification and initial structure-function characterization of ShuT (Shigella heme uptake protein) from Shigella dysenteria. Also generated new mutants for ShuT in the ligand-binding site to better understand the kinetics of hemebinding and release and generated anti-ShuT antibodies to study in-vivo expression levels in a siderophoredeficient mutant. This work resulted in a journal publication in Biochemistry in 2005.

University of London, London (UK). Ph.D. Student, University of London, Kings College, London, October 1997- April 2002. Project was concerned with elucidating the unknown structure, biochemistry and molecular biology of the secreted fungal enzyme copper-enzyme (laccase) from a leaf-litter degrading fungus M. galopus found in UK woodlands. Methods involved optimising fungal mycelium growth for enzyme production in various media and using various laccase enzyme inducers, Assaying various ligninolytic enzymes to quantify specific secreted protein like laccases, peroxidases, cellulases, mannanases and xylanases using spectroscopic and oxygen-rank electrode measurements. Developed a new technique for genomic DNA extraction from needle-litter colonised by M. galopus. Also modelled PCR-derived laccase fragment to determine amino acid/overall structural similarity of this novel laccase to other known basidiomycete laccases. This work resulted in a journal publication in Mycological Research in 2003. SCHOLARSHIPS AWARDED 1999-2000: NERC, Center for Ecology and Hydrology, Merlewood (UK), CEH-CASE studentship. 1998-2000: Overseas Research Awards Scheme (ORS) scholarship. 1997-2001: Kings College London studentship Scheme B scholarship. TECHNICAL SKILLS Biochemistry: Protein purification (Column chromatography including ion exchange, gel filtration, FPLC (AKTA system), affinity purification (Ni columns)). Radioactive assays using P32-ATP, SDS-PAGE, Western blotting, Zymograms, immunoprecipitation (IP), Co-IP, Circular Dichroism (CD), UV-Vis spectroscopy, EMSA (Electrophoretic Mobility Shift Assay), and ELISA. Molecular Biology: DNA extraction from fungal, bacterial and mammalian sources, bacterial transformation, mammalian transfections. PCR methodologies to create genomic cDNA library, gel electrophoresis, cloning, screening of phage libraries, site-directed mutagenesis, DNA footprinting, Chromatin immunoprecipitation assay (ChIP), real-time PCR (RT-PCR, qRT-PCR), Northern and Southern blotting. Cellular Assays: proliferation, growth, MTT, Scratch (wound-healing), Boyden Chambers invasion assays Modelling: Using Ras-Mol to investigate protein structure. Cell Culture: Extensive mammalian cell culture techniques known. Making transient and stable cell-lines, screening cultures. Basic FACS knowledge and fluorescence microscopy also known. Expertise in animal work: Worked with a animal (mice) model system of cancer. Presentation and Analysis: Proficient with Microsoft office, basic statistics and NCBI databases. OTHER SKILLS Ability to collaborate: I have collaborated with University faculty (Dr. R. Lapidus) for mouse work, and other department faculty for technical knowledge (cell sorting), skills and equipment use. Team leadership: Have effectively managed my own projects as well as delegated work for research technicians, fellow postdoc fellows and students. Mentoring: I have mentored, supervised and guided 3 research technicians in their projects, along with 5 graduate students, 7 summer interns, 3 medical fellows and 3 MSc students both at a technical level and also day-to-day activities to meet project deadlines. This has lead to successful completion of their projects (incl. PhDs), poster presentations and papers. Analytical and communication skills: I have analysed complex data sets critically, and presented findings at regular lab meetings, departmental meetings and poster presentations. This has also lead to paper publications and grants reports. I have also headed/collaborated in issues related to equipment use and management on a departmental basis. PROFESSIONAL MEMBERSHIPS 2009 to present: American Association for Cancer Research.

1994 to present: The Institute of Biology (UK). Arundhati Ghosh pg. 2

PUBLICATIONS Asrani, K., Brown, S.A.N., Tran, N.L., Ghosh, A., and Winkles, J.A. (2011). Constitutive and HRG1-Stimulated HER2 Signaling in Breast Cancer Cells Increases the Expression of Fn14, a Critical Regulator of Cell Invasive Capacity. (Manuscript in preparation). Ghosh, A. and Hamburger, A.W. (2011). The Regulation of Tamoxifen Sensitivity by a PAK1-EBP1 signaling pathway in ErbB2 overexpressing Breast Cancer Cells. Manuscript submitted to the British Journal of Cancer and under revision.

Hamburger A, Ghosh A, Awasthi S. PA2G4 (proliferation-associated 2G4, 38kDa). Atlas of Genetics and Cytogenetics Oncology and Haematology. September 2011. URL : http://AtlasGeneticsOncology.org/Genes/PA2G4ID41628ch12q13.html
Brown, S.A.N., Ghosh, A., and Winkles, J.A. (2010). Full-length membrane-anchored TWEAK can function as a juxtacrine signalling molecule and activate NF-kappaB pathway. Journal of Biological Chemistry 285 (23); pg 17432-17441. Cooper, Z.A.*, Ghosh, A.*, Gupta, A., Maity, T., Benjamin, I.J., Vogel, S., Hasday, J.D. and Singh, I.S. (2010). Febrile range temperature modifies cytokine gene expression in LPS-stimulated macrophages by differentially modifying NF-{kappa}B recruitment to cytokine gene promoters. American Journal of Physiology - Cell Physiology) 298 (1); c171- c181. *Equal authorship. Eakanunkul, S, Lukat-Rodgers, G.S., Sumithran, S., Ghosh, A., Rodgers, K.G., John H. Dawson, and Wilks, A. (2005). Characterization of the Periplasmic Heme-Binding Protein ShuT from the Heme Uptake System of Shigella dysenteriae. Biochemistry 44 (39); pg 13179-13191. Ghosh A, Frankland J.C., Thurston C.F., Robinson, C.H. (2003). Enzyme Production by Mycena galopus mycelium in artificial media and in Picea sitchensis F1 horizon needle litter. Mycological Research 107 (8); pg 996-1008. PRESENTATIONS

Awasthi, S., Ghosh, A., Wilson, G.M., Hassel, B.A. and hamburger, A. (2011). The ErbB3/4 receptor ligand Heregulin negatively regulates ErbB2 and erbB3 receptor levels. AACR Annual Meeting. March 31-April 4, 2012. Chicago, IL.
Cooper, Z., Ghosh, A., Gupta, A., Maiti, T., Benjamin, I. J., Vogel, S.N., Hasday, J.D., and Singh, I.S. (2009). Febrile range temperature modifies cytokine gene expression in LPS-stimulated macrophages without affecting upstream signalling events. 31st Annual Graduate Research Conference. University of Maryland, Baltimore. Ghosh A., Singh, I.S and Hasday, J.D. (2007). Febrile range hyperthermia represses TNF expression but does not affect Toll-like receptor signaling cascades upon LPS stimulation in murine macrophages. Experimental Biology meeting abstract (Section: cytokine and growth factor signaling). Eakanunkul, S, Ghosh, A., Lukat-Rodgers, G.S., Sumithran, S., Rodgers, K.G., John H. Dawson, and Wilks, A. Characterization of the periplasmic heme-binding protein ShuT from Shigella dysenteriae. (2006). 28th Annual Graduate Research Conference. University of Maryland, Baltimore. Eakanunkul, S., Ghosh, A., Lukat-Rodgers, G.S., Sumithran S., Rodgers, K.R., Dawson, J. H. and Wilks, A. (2005). Characterization of the periplasmic heme-binding protein, ShuT from Shigella Dysenteriae. 27th Annual Graduate Research Conference. University of Maryland, Baltimore. Ghosh, A., and Thurston, C. (1998). M. galopus laccase production in different media. Postgraduate Research Day. Kings College London (UK). REFERENCES

Provided upon request.

Arundhati Ghosh

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