BIS101/Draper Week#3 suggested problems and homework Problems suggest for the whole class these are not

t to be turned in, but are for practice/study aid. 8th edition Chapter 4: Chapter 5: 9th edition Chapter 4: Chapter 5: 10th edition Chapter 4: Chapter 5: 1, 3, 6, 10, 11, 18, 32, 35, 36 6, 8, 13, 17, 21, 23, 26 1, 3, 6, 10, 11, 18, 46, 49, 50 6, 8, 13, 17, 21, 23, 27 12, 14, 17, 21, 22, 29, 56, 59, 60 24, 26, 31, 35, 39, 41, 45

101D assignment #3 is listed on the next page (due the week of Jan 30). These questions are taken directly from past test. The key to the 101D assignment will be posted on SmartSite Feb 3 after 1:00pm.

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BIS101D/Draper Assignment#3 Due week of Jan 30

Name:_____________________________

1. After determining how many genes you have identified in the life-span screen, you turn your efforts to mapping the genes onto the worm genetic map. You start with the first complementation group that you have designated to be mutant alleles of the gene you now call age-1. Your preliminary mapping has determined that age-1 maps to linkage group II of the nematode genome. You next use three-factor mapping to refine the map position of age-1 on LG II. Your preliminary results suggest that age-1 is likely to map between the mutations dpy-5 (a recessive mutation that results in dumpy (i.e. short and squat) worms, and unc-11 (a recessive mutation that results in uncoordinated worms). The three point testcross you perform is as follows: Cross: female dpy+ unc age+ dpy unc+ age X dpy unc age male dpy unc age

The results of this test cross are as follows: wild-type dpy dpy age dpy unc dpy unc age unc unc age age Total 121 4 999 53 117 991 3 49 2337

a. (8 pts) Using the data above, determine the map order and the genetic distance between the three genes. Show your work.

b. (4 pts) Is there interference in this region of the genome? Calculate interference I = 1- coefficient of coincidence = (observed DCO)/(expected DCO) to determine your answer.

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2. The phage P1 is used as a generalized transducing phage in an experiment involving a donor strain of E. coli with the genotype leu+ phe+ ala+ and a recipient strain that is leuphe- ala-. In separate experiments, transductants are selected leu+ in experiment A, for phe+ in experiment B, and for ala+ in experiment C. Following selection, transductant genotypes for the unselected markers are identified. a. What compound(s) is/are added to the minimal medium to select for transductants in experiment A?

Selection experiment results below show the frequency of each genotype. Experiment A phe- alaphe+ alaphe- ala+ phe+ ala+ 26% 50% 19% 3% Experiment B leu- alaleu+ alaleu- ala+ leu+ ala+ 65% 48% 0% 4% Experiment C leu- pheleu+ pheleu- phe+ leu+ phe+ 71% 21% 0% 3%

b. Use the information in the table above to determine the order of genes on the donor chromosome.

c. Diagram the crossover events that form the most common cotransductant in experiment A.

d. In experiment B, why are there no transductants with the genotype leuala+?

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3. In a transduction experiment, progeny phage produced by the infection of a donor E. coli strain with the genotype met+ ala+ trp+ are used to transduce a recipient bacterial strain with the genotype met- ala- trp-. The treated recipient cell population is plated on minimal medium supplemented with alanine and tryptophan. A large number of colonies are obtained. a. What are the possible genotypes of the colonies growing on this initial plate?

b. Are the colonies on the initial plate transductants? Explain your reasoning.

The colonies on the initial plate are replica-plated onto to three different media. Plate A is minimal medium supplemented with tryptophan, plate B is minimal medium supplemented with alanine and, plate C is minimal medium only. c. What genotypes are possible among colonies that grow on each of the three test plates? Place your answers on the lines below. Plate A _______________________________ Plate B _______________________________ Plate C _______________________________ The growth results on the three test plates are as follows, plate A contains 112 colonies, plate B contains 11 colonies, while plate C contains no colonies at all. d. Use this information to determine the actual genotypes of colonies growing on plate A and plate B. Place your answers on the lines below. Plate A _______________________________ Plate B _______________________________

Copyright UC Regents Davis campus, 2005-12. All Rights Reserved. May not be re-distributed without prior written consent of course instructor.