Jamonline /Jan-Feb 2(1) / 103108 Research Article

Lakshman Raju B & Rajesh G

Journal of Atoms and Molecules

An International Online Journal
ISSN 2277 1247

ESTIMATION OF GLIPIZIDE IN COMMERCIAL DRUGS BY RP HPLC

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Lakshman Raju Badugu1*, Rajesh Gunti1 M.Sc 2008 10 Batch Students, Department of Chemistry, Bhagwan Sri Satya Saibaba PG College, Tadikonda, A.P, India Revised on: 18-02-2012 Accepted on: 23022012

Received on: 10-01-2012 Abstract:

Glipizide is used together with diet and exercise to reduce blood glucose in patients with type 2 diabetes. Recommended starting dose is Glipizide 5 mg, taken once daily before breakfast. For elderly people or people with liver problems, the recommended starting dose is lower. A Glipizide overdose can cause low blood sugar (hypoglycaemia), Sweating Shakiness, Extreme hunger, Dizziness, Cold sweats, Blurry vision. The present study is used for the determination of the assay of Glipizide in the commercial samples. Among those Glicept contains maximum amount of Glipizide. Key Words: Glipizide, HPLC, Assay, Otsira, Glicept. Introduction: Glipizide is * Corresponding author Lakshman Raju Badugu, Email: lakshman.raju678@gmail.com Tel: + 91 9866508397 an oral medium-to-long drug from

acting anti-diabetic

the sulfonylurea class. It is classified as a second generation sulfonylurea, which means that it undergoes enterohepatic circulation. The structure on the R2 group is a much larger cyclo or aromatic group compared to the 1st generation sulfonylureas.

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Jamonline /Jan-Feb 2(1) / 103108 Drug Stricture

Lakshman Raju B & Rajesh G gas. Skin rashes can occur and cause itching, hives, or a diffuse measles-like rash. Rare but serious side and a effects low include sodium

hepatitis, jaundice,

concentration. Glipizide may also cause hypoglycemia. The risk of hypoglycemia increases when glipizide is combined with other glucose reducing agents. Figure 1.1: stricture of Glipizide. IUPAC Name: N-(4-[NMaterials and methods Methanol, Acetonitrile and Water used were HPLC Grade and were purchased from MERK Chemicals PVT LTD. For analysis of Drug, PEAK 7000 isocratic HPLC with fallowing configuration is used. Peak 7000 delivery system. Rheodyne manual sample switch (77251). Analytical column Kromosil 100-5 injector with

(cyclohexylcarbamoyl)sulfamoyl]phenethyl)5-methylpyrazine-2-carboxamide Insulin is a hormone that is made in the pancreas that, when released into the blood causes cells in the body to remove sugar (glucose) from the blood and reduces the formation of glucose by the liver. Patients with type 2 diabetes have high glucose (sugar) levels in their blood because the cells in their bodies are resistant to the effect of the insulin, and the liver produces too much glucose. In addition, in type 2 diabetes the pancreas is unable to produce the increased amounts of insulin that are necessary to overcome the resistance. Glipizide reduces blood glucose by stimulating the pancreas to produce more insulin. Glipizide is not a cure for diabetes. The usual starting dose when using immediate release tablets is 5 mg administered 30 minutes before a meal. The maximum dose is 40 mg daily. Doses higher than 15 mg per day should be divided. Side effects and

C18.250*46mm. Preparation of Mobile phase The suitable mobile phase for the estimation of Glipizide consist of Methanol : Acetonitrile : Water 40:40:20(v/v/v). Preparation of Standard Solution The standard drug sample were obtained from Torrent pharma, India. About 1 mg of Glipizide standard was accurately weighed and transferred into 10ml volumetric flask and makeup with sufficient mobile phase. The volumetric flask containing standard solution kept for sonication for 10min and is filtered

include headache,

dizziness, diarrhea,

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Jamonline /Jan-Feb 2(1) / 103108 through 0.45um Nylon 6,6 membrane filtered paper. Preparation of Sample Solution: Different formulations of Glipizide were purchased from local market and is dissolved in appropriate amount of mobile phase , the solution kept for sonication for 10min and is filtered through 0.45um Nylon 6,6 membrane filtered paper analysis. Procedure for analysis: Optimized chromatographic conditions for analysis of Glipizide are given the table 1. With the optimized chromatographic and is used as sample for Conclusion:

Lakshman Raju B & Rajesh G

The concentration of Glipizide in Glicept is maximum when compare to other samples, It contain 91.4% of Glipizide, where as the concentration is minimum inGlimet, it contain 63% of Glipizide. Maximum number of impurities were detected in Gynase. The over dosage of Glipizide causes side effects like low blood sugar (hypoglycemia), Sweating Shakiness,Extreme hunger, Dizziness,Cold sweats, Blurry vision. so people can aware of these chemical composition in the

commercially available forms and use the proper dosage for their better recovery and reduce the side effects. References: 1 Swaroop R. Lahoti, Prashant K, Puranik, Ashish, A. Heda, Development and Validation of RP-HPLC Method for Analysis of Glipizide in Guinea Pig Plasma and its Application to

conditions for the HPLC were set for Glipizide, a study base line was recorded. After the stabilization of the base line for about 30min, successive aliquots of the sample solution were injected separately and chromatograms were recorded. This

procedure was applied for every commercial sample. 2 Results and discussion: The standard chromatograms and sample were shown in figure 1.2 and 1.3. The assay for different commercial samples of Glipizide were estimated by using the fallowing formula, and results were shown in table 1.A 3

Pharmacokinetic Study. Hkan Emilsson, High-performance liquid chromatographic determination of

glipizide in human plasma and urine, Journal of Chromatography B: Biomedical Sciences and Applications Volume 421, 1987, Pages 319-326. Bae, Jung-Woo; Kim, Nam-Tae; Choi,

Chang-Ik; Kim, Mi-Jeong; Jang, ChoonGon; Lee, Seok-Yong, HPLC Analysis of Plasma Glipizide and its Application to Pharmacokinetic Study, Journal of Liquid

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Jamonline /Jan-Feb 2(1) / 103108 Chromatography & Related Technologies, Volume 32, Number 13, January 2009. 4 Gupta S, Bansal G, Validated stabilityindicating HPLC-UV method for

Lakshman Raju B & Rajesh G

simultaneous determination of glipizide and four impurities, J AOAC Int. 2011 Mar-Apr;94(2):523-30. 5 S.D. Jadhav, A.V.Chandewar,R.L.

Bakal,A. Dewan, Development Of Hplc Method And Validation For The

Estimation Of Glipizide And Metformin Hydrochloride In Combined Dosage

Form.International Journal of Pharma Vol 2, No. 2,March -June 2011 6 Xiao-Hua Zhao, Bo Song, Da-Fang

Zhong, Shu-Qiu Zhang, Xiao-Yan Chen, Simultaneous determination of metformin and glipizide in human plasma by liquid chromatography-tandem mass

spectrometry, Yao xue xue bao Acta pharmaceutica Sinica (2007),Volume: 42, Issue: 10, Page s: 1087-1091.

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Jamonline /Jan-Feb 2(1) / 103108 S.No 1 Parameter Mobile phase

Lakshman Raju B & Rajesh G Condition Methanol:Acetonotrile:Water (40:40:20)

2 3 4 5 6 7 8 9

Column Wavelength Injection volume Run time Flow rate Retention time Temperature Pump pressure

Kromosil C18 256nm 20ul 6 min 1ml/min 3.62 Ambient 3000psi

Table 1: Optimim Conditions of Chromatography

Figure1.2: standard chromatogram of Glipizide. All rights reserved 2011 www.jamonline.in 107

Jamonline /Jan-Feb 2(1) / 103108

Lakshman Raju B & Rajesh G

Figure1.3: sample chromatogram of Glipizide (Brand name:Bigunyl).

S.No 1 2 3 4 5 6 7

Sample Standard Otsira Bigunyl Glicept Glimet Gynase Dlipizide

RT 3.62 3.60 3.79 4.32 4.12 3.81 3.58

%Assay 100 86.109 70.101 91.398 62.914 75.627 76.516

Table 1.A: Assay results of Glipizide in different commercial formulations.

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