Resume

Sharmin Sultana
Phone Number : E-mail address : Mailing Address : +1 306-261-2925 sharmin76du@gmail.com 113, Avenue U South Apartment: 15 Saskatoon, Saskatchewan S7M 3B8, Canada

OBJECTIVE
I am highly motivated person with the ability of working hard by sincerity, creativity, dynamism and skill to develop new ideas, leading them to successful outcomes, looking for a position to apply my skills in a reputed organization.

CURRENT STATUS
Staying in Canada with valid work permit.

EDUCATION
Exam Master of Science (M.Sc) in Biochemistry and Molecular Biology Bachelor of Science (B.Sc) in Biochemistry and Molecular Biology Higher Secondary Certificate (HSC) Secondary School Certificate (SSC) Institution University of Dhaka Yea r 2009 Grade First Class 6th position First Class 7th position A+ (5.00 out of 5.00) A (4.75 out of 5.00)

University of Dhaka

2008

Ideal School and College, Motijheel Adarsha Girls High School

2004 2002

EMPLOYMENT HISTORY
06/2011 Present: Research Officer, Immunology laboratory, LSD, ICDDR,B, Bangladesh 09/2009 01/2011: Elective Research Fellow, Immunology laboratory, LSD, ICDDR,B, Bangladesh 04/2008 05/2009: Fellow Student, Molecular Biology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh

RESEARCH EXPERIENCES
I have done research work in different field of Immunology laboratory at the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B) under the supervision of Dr. Firdausi Qadri from November09 to December' 11. My Masters thesis work which was titled Detection of genetic diversity of heat labile toxin (LT) expressed by enterotoxigenic Escherichia Coli (ETEC) strains isolated from human. Main target of this work was vaccine development against ETEC diarrhea. After my thesis work I have done Mismatch Amplification Mutation assay PCR (MAMA PCR), AKI ELISA and various phenotypic tests like- hemolysis, chicken cell agglutination (CCA) test, lysis by classical phage-IV, lysis by El Tor phage 5, sensitivity to polymyxin B assay for the identification of Classical and El Tor biotypes of Vibrio Cholerae. Main purpose of this work was to detect low toxin producing altered El Tor strains for the development of vaccine against cholera. Currently I am doing Vibriocidal assay for the detection of antibody titre in plasma against Vibrio Cholerae. As I am so passionate about Molecular Biology, during my Bachelor degree I worked on the Molecular Biology laboratory in my department (Department of Biochemistry and Molecular Biology, University of Dhaka) as a fellow student and learnt numerous basic molecular biological techniques.

LABORATORY EXPERTISE
Molecular Biological Techniques: DNA, RNA, plasmid and protein extraction, PCR (Polymerase Chain Reaction), RT-PCR (Real Time PCR), gel electrophoresis, Polyacrylamide gel electrophoresis (PAGE), Restriction digestion of genomic DNA, Western blotting, SDSPAGE and other basic molecular biological techniques. Basic Microbiological and Biochemical Techniques: Bacterial culture, Toxin assay, Bacterial growth curve construction, common tests with microorganism, thin layer chromatography (TLC), gel filtration chromatography, Column chromatography and basic biochemical assay techniques. Immunological Techniques: Blood cell separation using different techniques, in vitro cell culture, ELISPOT (Enzyme-linked Immunospot Assay), ELISA (Enzyme-linked Immunosorbant Assay), Antibody in lymphocyte supernatant assay (ALS),T cell proliferation Assay using triated thymidine, Cytokine Bead Array (CBA), FASCIA, Immonodot blot assay, Widal agglutination assay, Flowcytometry (FACS), Antigen extraction and other general techniques on Immunology. Bioinformatics: Organization of data bases: genome, nucleotide and protein (primary, secondary and composite) sequences, macromolecular structure, integrated database, bibliographic databases, and gene expression data.

Search and analysis: Text based searching; Nucleotide database searching (using Blast, Fasta, EMBL-EBI, DDBJ etc): retrieval of specific genes(s) from database; sequence alignment and estimation homology (using CLUSTAL. W etc): searching restriction sites within the sequence; identification of transcription termination signal; primer design; Protein database searching (using SWISSPROT, PDB, PRF, PIR etc): amino acid sequence alignment and homology; conserved sequence; protein motif, secondary structure analysis: hydropathy analysis; dendogram construction. Microarray database

SKILLS
Computer Literacy Working Experience on Windows Machine. Microsoft Office (Word, Excel, Power point) Database knowledge (Microsoft Access) Programming language C. Web Browsing, internet access, email etc. Photo shop.

Language Proficience y

Bengali (Native) Hindi English (fluent in Speaking, Listening, Reading and Writing)

SELF APPRAISAL
I am very hard working, capable of capturing things quickly and thus able to take decision quickly that helps me to solve any kind of problem, adaptive to any environment, smart, fluent in English, young, energetic, amiable, honest, trustworthy, friendly and also I have good academic background.

WORK EXPERIENCE
I have 1-year time work experience as a sales manager in Desi Dosh at Bashundhara City Shopping Mall in Bangladesh. 1.5 years internship experience in an international Research Institute ICDDR, B. 6 months work experience in ICDDR, B.

WORK AVAILABILITY
Full time Available immediately at any time.

REFERENCE
Azharul Islam Research associate Vaccine and Infectious Disease Organization (VIDO) University of Saskatchewan

Former postdoctoral fellow Saskatchewan Health Research Foundation (SHRF) Office Phone: (306) 966-7494 Lab Phone: (306) 966-7486 E-mail: azhar_is2003@yahoo.com