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fuel ethanol

Making cellulosic ethanol a reality

Novozymes CellicTM product family for cellulosic ethanol currently has the best costperformance ratio in the industry. These products move the industry a step closer to making cellulosic ethanol a commercially viable reality.
Efficiency Best costperformance ratio in the industry
Cellic CTec, a unique cellulase complex for cost-effective hydrolysis Proprietary hydrolysis-boosting protein Hydrolysis boost option with hemicellulase Cellic HTec High performance with low dosage

Versatility Proven on several feedstocks

High conversion efficiency on acid and neutral pre-treated feedstocks Product combination option for alkaline pre-treated materials Proven on corn stover and cobs, sugarcane bagasse, wheat straw, woody feedstocks, and a number of other agricultural residues

Consistency A concentrated and stable formulation

Low-dose, easy-to-use, highly concentrated formulation Proven performance in partnership operations Low storage and shipping costs

Trust Novozymes is the partner for the future of fuel

We are open to all types of enzymatic conversion technologies We work on a variety of common feedstocks We can provide extensive technical support We have mobilized extensive R&D resources for the industry

novozymes is the world leader in bioinnovation. together with customers across a broad array of industries we create tomorrows industrial biosolutions, improving our customers business and the use of our planets resources.

Performance boost with CellicTM CTec

New enzyme Previous enzyme available % Conversion to glucose (72 hours) 100 80 60 40 20 0 0 0,04 0,06 0,12 0,16 Enzyme dosage (ml of product/g of cellulose) 0,20

CellicTM CTec works on many feedstocks

Sugarcane bagasse Corn fiber 100 80 % Glucose yield 60 40 20 0 Corn stover Wood pulp

15 mg enzyme/g TS

15 mg enzyme/g TS

15 mg enzyme/g TS

50 mg enzyme/g TS

Pretreated feedstock

Fig. 1. % cellulose conversion vs ml of product pr g of cellulose. 72-hr hydrolysis at 50 C, pH 5.0, 7% cellulose.

Fig. 2. Glucose formation from pretreated feedstocks. Hydrolysis for 3 days, 50 C, pH 5.0.

Cleaning cellulose with CellicTM HTec to boost hydrolysis

Fig. 3. Dark field microscope photo of NREL PCS before (left) and after (right) treatment with CellicTM HTec, showing a clean, unobstructed cellulose fiber after treatment. Removal of lignin and hemicellulose from the fiber structure makes hydrolysis easier for the cellulase enzymes.

Novozymes A/S Customer Communications No. 2009-03043-01

For more information visit www.bioenergy.novozymes.com

Novozymes North America, Inc. 77 Perry Chapel Church Road Franklinton, NC 27525 USA Tel. +1 919 494 3000 Fax +1 919 494 3450 Novozymes France S.A. Immeuble Challenge 92 79, Avenue Franois Arago 92017 Nanterre Cedex France Tel. +33 146140746 Fax +33 146140766 Novozymes (China) Investment Co Ltd. 14 Xinxi lu Shangdi Zone Haidian District 100085 Beijing China Tel. +86 10 6298 7888 Fax +86 10 6298 1283 Novozymes A/S Krogshoejvej 36 2880 Bagsvaerd Denmark Tel. +45 4446 0000 Fax +45 4446 9999 fuel-ethanol@novozymes.com www.novozymes.com
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