IBIS Data Acquisition Training Guide

Calibrating the Microscope

Each time a user logs on to the IBIS application, an automatic calibration program checks the microscope motor, light, zoom and focus mechanisms. For IBIS to perform this calibration, it is important that the cartridge case holder is free of obstacles and that the light conversion device is in the shroud position. In addition to this automatic calibration, it is also recommended you perform a manual microscope calibration on a regular basis. The DAS Microscope Calibration program that comes installed on the IBIS machine calibrates the BRASSCATCHER portion of the microscope. This calibration program tests motor switches, lights and zoom lens magnifications of BRASSCATCHER. To open the calibration software, double click the Microscope Calibration icon on the IBIS desktop. The calibration software activates the microscope and displays the cameras view of the calibration pin. The calibration pin is located towards the rear of the cartridge case holder and is used as a reference point for the calibration. By using the light and focus scroll bars, verify/adjust the following parameters: Optimal light level: Verify that there is no washout in the image. Focus: Verify that the image is in focus. Magnification: Set magnification so that the calibration ring is within the two red rings (refer to example above). Do not move the red rings!

Module 13: Administration

The manual calibration process, when carried out regularly, ensures better system performance. Adjustments may not be necessary; you need only make adjustments to the light, focus, and magnification when needed. If adjustments are made, click the checkmark button to accept the new settings. If no adjustments are needed, click the Cancel button to exit and keep the current settings. It is recommended you perform a manual calibration of the microscope once a week. A common practice is to perform the calibration after switching the back-up tapes each Monday morning. It is good practice to keep a log of the calibration. This log could include such details as: who performed the calibration, the date of the calibration, and the adjustments made.

IBIS Data Acquisition Training Guide

Demonstration

Calibrating the Microscope

The following demonstrates how to calibrate a microscope: 1. Log on to Windows NT. 2. In the upper monitor, double click the Microscope Calibration icon. The Calibration window opens. 3. Review the following and make adjustments as needed: a. Motor position: the center calibration pin is positioned between the two red rings. Make adjustments using only the motors; do not move the rings. b. Light: there should be no washout in the image. c. Focus: the image should be in focus. d. Magnification: The calibration pin should be at a magnification where it is centered between the two red rings. Make adjustments to the magnification only; do not move the rings! 4. Click the checkmark button to save the new settings. If you do not need to make any calibration adjustments, click the Cancel button to exit the program.