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(a) True (b) False 2. What enables scientists to take nucleotide fragments from other DNA and reassemble fragments into a new nucleotide sequence? (a) Enzyme DNA technology (b) Enzyme technology (c) Recombinant DNA technology (d) Recombinant enzyme technology 3. What is used to cut DNA double-helix strand DNA along the exterior of the strand? (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence 4. What is the particular nucleotide sequence of a double-helical segment called? (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence 5. What is the end (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence of the cut of a double-helical segment called?
6. What results when two incisions are made in a double-helical segment? (a) Overhang (b) Restriction enzymes (c) Restriction fragment (d) Recognition sequence 7. The four nucleotides are adenine (A), cytosine (C), guanine (G) and thymine (T). (a) True (b) False
8. What is another (a) Vector (b) Plasmid (c) Restriction endonucleases (d) Agarose gel
name
for
restriction
enzyme?
9. The Southern blotting technique is used for detecting specific restriction fragments. (a) True (b) False 10. Scientists synthesize fragments of DNA and RNA using a process known as polymerase chain reaction (PCR). (a) True (b) False
12) Which is the most specific recombinant DNA library? a) b) c) d) Genomic Protein cDNA chromosomal
13) Recombinant DNA research is dependent on ________ a) b) selectively replicates RNA probes and selectively replicates DNA
c) d)
15) The polymerase chain reaction is a technique that __________. a) b) c) d) selectively replicates RNA probes and selectively replicates DNA selectively replicates DNA probes DNA
16) Chromosome walking is a technique used to __________. a) b) to another. c) d) move chromosomes around the nucleus move a fragment of chromosomal DNA from one area of a chromosome
recombination between chromosomal DNA of two different species a method used to locate a gene using a set of clones from a DNA library
21) ________ is defined as the use of living organisms for the welfare of
23) ________ are small, extra circular DNA molecules found in some bacteria.
26) The group of enzymes that are used to cut up the DNA molecule are called
restriction enzymes DNA ligase enzyme DNA polymerase enzyme none of them
bacteriophage
30) When a foreign gene is inserted to natural plants they are called ________.
DNA finger prints DNA fragment length DNA length measurement Restriction fragment length polymorphism
37) The cell which contains all the genetic potential of the organism is called