Product Information Sheet for ATCC 13047

COLLECTION OF BACTERIA
ATCC NUMBER: 13047 Please see the enclosed Material Transfer Agreement (MTA) for further details regarding the use of this product. The MTA is also available on our Web site at www.atcc.org. PROPAGATION PROCEDURE: 1. Open vial according to enclosed instructions. 2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet. Aseptically transfer this aliquot back into the broth tube. Mix well. Use several drops of the suspension to inoculate a #3 agar slant and/or plate. Incubate the tubes and plate at 30oC for 24 hours.

ORGANISM: Enterobacter cloacae subsp. cloacae Type strain. Deposited as Aerobacter cloacae. Quality control strain for API, BBL, and Sensititre products. Purified genomic DNA of this strain is available as ATCC 13047D. CITATION OF STRAIN: If use of this strain results in a scientific publication it should be cited in that manuscript in the following manner: Enterobacter cloacae subsp. cloacae ATCC 13047. ATCC MEDIA: #3 Broth: Nutrient Broth (BD 234000) #3 Agar: Nutrient Agar (BD 213000) CONDITIONS: Temperature: 30C Atmosphere: Aerobic BIOSAFETY LEVEL: 1 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. from the U.S. Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes for Health. The entire text is available online at www.cdc.gov/od/ohs/biosfty/bmbl5/bmbl5toc.htm. ATCC WARRANTY: The viability of ATCC products is warranted for 30 days from the date of shipment. If you feel there is a problem with this product, contact Technical Services by phone at 800-6386597 or 703-365-2700 or by e-mail at tech@atcc.org. Or you may contact your local distributor. DISCLAIMERS: This product is intended for laboratory research purposes only. It is not intended for use in humans. While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, and use. ATCC is not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of cultures.
ATCC (American Type Culture Collection) P.O. Box 1549 Manassas, VA 20108 USA www.atcc.org

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NOTES: Two colony types observed on #3 agar are a) entire, glistening, circular, smooth, low convex, and translucent; b) small, entire, circular, smooth, white, convex, and opaque. Additional information on this culture is available on the ATCC web site at www.atcc.org. REFERENCES: 1. Skerman VB, et al. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30: 225-420, 1980. 2. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 55: 1395-1397, 2005. PubMed: 16014455 3. Reassignment of Enterobacter dissolvens to Enterobacter cloacae as E. cloacae subspecies dissolvens comb. nov. and emended description of Enterobacter asburiae and Enterobacter kobei . Syst. Appl. Microbiol. 28: 196-205, 2005. PubMed: 15900966 ATCC, 2008. All rights reserved. ATCC is a registered trademark of the American Type Culture Collection. Updated (07/07) MVB/AS

800-638-6597 or 703-365-2700 Fax: 703-365-2750 E-mail: tech@atcc.org Or contact your local distributor.

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