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active sites available, an increase in substrate concentration will lead to an increase in enzyme activity. 3.6.4: Define denaturation. Define means to give the precise meaning of a word, phrase or physical quantity. - Denaturation is changing the structure of an enzyme (or other protein) so it can no longer carry out its function. 3.6.5: Explain the use of lactase in the production of lactose-free milk. Explain means to give a detailed account of causes, reasons or mechanisms. - Lactose is the sugar found in milk. It can be broken down by the enzyme lactase into glucose and galactose. However some people lack this enzyme and so cannot break down lactose leading to lactose intolerance. Lactose intolerant people need to drink milk that has been lactose reduced. Lactose-free milk can be made in two ways. The first involves adding the enzyme lactase to the milk so that the milk contains the enzyme. The second way involves immobilizing the enzyme on a surface or in beads of a porous material. The milk is then allowed to flow past the beads or surface with the immobilized lactase. This method avoids having lactase in the milk.