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EXPERIMENT 4 1. A water sample in a clean 300 mL BOD bottle was collected.

The sample was allowed to overflow the bottle for 2-3 minutes to ensure the air bubbles were not trapped. 2. Next the sample was added with one Manganous Sulfate Powder Pillow and one Alkaline IodideAzide Reagent Powder Pillow. 3. A stopper was inserted immediately without trapping air inside the bottle. The bottle was inverted several times to mix the solution. 4. After the bottle was inverted several times,waited until the floc settles and a clear solution were obtained. 5. The stopper was removed and the content were added with one Sulfamic Powder Pillow. The stopper was replaced without trapping the air inside the bottle and then again the prepared sample was inverted several times. 6. A sample volume and Sodium Thiosulfate Titration Cartridge were selected corresponding with expected dissolved oxygen (DO) concentration. 7. Then a clean delivery tube was inserted into the titration cartridge to the titrator body. After that, the delivery knob was turned to inject a few drops of titrant. The counter is resetted to zero and the tip was wiped. 8. A graduated cylinder was used to measure the sample volume and the sample was transferred to 25mL Erienmeyer flask. 9. The delivery tube was placed into the solution and the flask is swirled while the solution was titrated with sodium thiosulfate to a pale yellow colour. 10. Next, two 1 mL dropper of Starch Indicator solution were added and the mixture was swirled. 11. The titration was continued to get a colourless end point. The number of digits required were recorded. 12. Finally, dissolved oxygen were calculated and being tabulated in a table.

EXPERIMENT 5 Experiment A: Chlorine, Total (0-2.00 mg/L) Using D PD Method 1. The stored program number for free and total chlorine (Cl2) powder pillow is entered. (8 0 ENTER) and display will show: Dial nm to 530 2. The wavelength dial is rotated until the screen shows the dial number that equals to the one that recommended. When the correct dial wavelength is dialled, the display will quickly show: Zero Sample then: mg/L Cl2 . 3. The 10-mL cell riser is inserted into the sample compartment. 4. A 10-mL sample cell is filled with 10-mL of sample. 5. One contents of DPD Total Chlorine Powder Pillow is added to the sample cell known as prepared sample. The sample then is covered with stopper and shaked for 20 seconds. Stopper is then removed. 6. The SHIFT TIMER button is pressed and a three-minute reaction period will begin. 7. Another sample cell known as the blank with 10-mL of sample is filled and then placed into the cell holder. The light shield then is closed. All these are done just after the timer beeps. 8. Zero button is pressed and the display will show: Zeroing then: 0.00 mg/L Cl2 9. Within three minutes after timer beeps, the prepared sample is place into the cell holder then the light shield is closed. 10.Read button is pressed and it will show: Reading .The result in mg/L Cl2 will appear and then recorded.

Experiment B: Chlorine, Free (0-2.00 mg/L) Using D PD Method 1. The stored program number for free and total chlorine (Cl2) powder pillow is entered. (8 0 ENTER) and display will show: Dial nm to 530 2. The wavelength dial is rotated until the screen shows the dial number that equals to the one that recommended. When the correct dial wavelength is dialed, the display will quickly show: Zero Sample then: mg/L Cl2 .

3. The 10-mL cell riser is inserted into the sample compartment. 4. A 10-mL sample cell is filled with 10-mL of sample known as the blank and then it is placed into the cell holder and the light is closed. 5. Zero button is pressed and the display will show: Zeroing then: 0.00 mg/L Cl2 6. Another cell is filled with the 10-mL of sample. 7. One contents of DPD Free Chlorine Powder Pillow is added to the sample cell known as prepared sample. The sample then is covered with stopper and shake for 20 seconds. 8. Immediately a minute after reagent addition, the stopper is removed. The prepared sample is place into the cell holder then the light shield is closed. 9. Read button is pressed and it will show: Reading .The result in mg/L Cl2 will appear and then recorded.

Experiment C: Sulfate (0 to 70 mg/L) Sulfa Ver 4 Method 1. The stored program number for sulfate (SO42-) powder pillow is entered. (6 8 0 ENTER or 9 ? ? ENTER) and display will show: Dial nm to 450 2. The wavelength dial is rotated until the screen shows the dial number that equals to the one that recommended. When the correct dial wavelength is dialed, the display will quickly show:Zero Sample then: mg/L SO42- . 3. A 25-mL sample cell is filled with 25-mL of sample. 4. One contents of Sulfa Ver 4 Sulfate Reagent Powder Pillow is added to the sample cell known as prepared sample. The sample is swirled to dissolve the mixture. A white turbidity is developed. 5. The SHIFT TIMER button is pressed and a five-minute reaction period will begin. The cell is allowed to stand undisturbed. 6. Another sample cell known as the blank with 25-mL of sample is filled and then placed into the cell holder. The light shield then is closed. All these are done just after the timer beeps. 7. Zero button is pressed and the display will show: Zeroing then: 0. mg/L SO42- .

8. Within five minutes after timer beeps, the prepared sample is place into the cell holder then the light shield is closed. 9. Read button is pressed and it will show: Reading .The result in mg/L SO42- will appear and then recorded.

Experiment D: Phosphorus, Reactive (0-2.50 mg/L PO43-) Using Phos Ver 3 (Ascorbic Acid) Method 1. The stored program number for reactive phosphorus, ascorbic acid method, powder pillows is entered. (4 9 0 ENTER) and display will show: Dial nm to 890 2. The wavelength dial is rotated until the screen shows the dial number that equals to the one that recommended. When the correct dial wavelength is dialed, the display will quickly show: Zero Sample then: mg/L PO43- PV 3. The 10-mL cell riser is inserted into the sample compartment. 4. A 10-mL sample cell is filled with 10-mL of sample. 5. One contents of Phos Ver 3 Phosphate Powder Pillow is added to the sample cell known as prepared sample. And then it is swirled immediately to mix. 6. The SHIFT TIMER button is pressed and a two-minute reaction period will begin. 7. Another sample cell known as the blank with 10-mL of sample is filled and then placed into the cell holder. The light shield then is closed. All these are done just after the timer beeps. 8. Zero button is pressed and the display will show: Zeroing then: 0.00 mg/L PO43- PV 9. The prepared sample is place into the cell holder then the light shield is closed. 10.Read button is pressed and it will show: Reading .The result in mg/L PO43- will appear and then recorded the results of forms P, PO43- and P2 O5 .

Experiment E: Iron, Total (0-3.00 mg/L) Using Ferro Ver Method 1. The stored program number for iron (Fe) Ferro Ver powder pillow is entered. (2 6 5 ENTER) and display will show: Dial nm to 510 2. The wavelength dial is rotated until the screen shows the dial number that equals to the one that recommended. When the correct dial wavelength is dialed, the display will quickly show: Zero Sample then: mg/L Fe FV. 3. The 10-mL cell riser is inserted into the sample compartment. 4. A 10-mL sample cell is filled with 10-mL of sample. 5. One contents of Ferro Ver Iron Reagent Powder Pillow is added to the sample cell known as prepared sample and then swirled to mix. 6. The SHIFT TIMER button is pressed and a three-minute reaction period will begin. 7. When the timer beeps, the display will show: mg/L Fe FV and another sample cell known as the blank with 10-mL of sample is filled. 8. Then placed into the cell holder. The light shield then is closed. 9. Zero button is pressed and the display will show: Zeroing then:0.00 mg/L Fe FV 10.Within, thirty minutes after timer beeps, the prepared sample is place into the cell holder then the light shield is closed. 11.Read button is pressed and it will show: Reading .The result in mg/L iron will appear and then recorded.

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