EXPERIMENT OF LIVING THINGS (CRICKET) RESPIRATION

Group 1 Member of Group : ~ Anggika Putra Iriandana ~ Aulia Choirina Putri ~ Dalilah Badzlin ~ Nur Muhammad Zain ~ Yusuf Ridho Hardiansyah

(04) (05) (06) (22) (29)

EXPERIMENT OF LIVING THINGS (CRICKET) RESPIRATION

Problem
1. How much oxygen that required for animal respiration in different number of animal? 2. What is the factor that influences the different of using the oxygen in the respirometer?

Purpose
1. To determine the amount of oxygen required for the use of breathing by the animal with different numbers of animal. 2. To be able to mention the factors that influence differences in the use of oxygen in the respirometer

Hypothesis
1. Greater the number of living thing, the amount of oxygen that needed for respiration is more. 2. The factors are amount of living thing in the respirometer and the amount of oxygen that absorbs by the insect, so the comparison of oxygen that used is different.

Variables
a) Manipulation Variable b) Control Variable c) Response Variable : Amount of Cricket : Time : Amount of oxygen that used

Basic Theory
Respiration Respiration in biology is the process of energy mobilization carried the bodies of living through a solution of high-energy compounds (SET) for use in performing the functions of life. In terms of daily life activities, respiration can be likened to breathing. However, the term includes the processes of respiration are also not covered in terms of breathing. Respiration occurs at all levels of living organisms, ranging from individuals to the smallest unit, the cell. If breathing is usually associated with the use of oxygen as a breaker compound, not only involving the oxygen respiration. Basically, respiration is the process of oxidation experienced by SET as chemical energy storage unit in living organisms. SET, such as sugar molecules or fatty acids, can be broken with the help of enzymes and some simple molecules. Because this process is an exothermic reaction (releases energy), the energy released was captured by ADP to form ATP or NADP or

NADPH. In turn, a variety of biochemical reactions endothermic (requires energy) supplied its energy needs from these last two groups of compounds. Most of which can be witnessed human respiration requires oxygen as oxydator. Such a reaction is called aerobic respiration. However, many processes that do not involve oxygen respiration, called anaerobic respiration. The most commonly known one is in the process of making alcohol by the yeast Saccharomyces cerevisiae. A variety of anaerobic bacteria using sulfur (or compounds) or some metal as an oxidant. Performed on the unit cell respiration. Respiration processes in eukaryotic organisms occurs in the mitochondria.

Vaseline

Vaseline is a composite object and semi-solid hydrocarbons (especially with the amount of carbon is higher than 25), which is promoted as an ointment. Vaseline is recognized by the U.S. Food and Drug Administration as a skin protectan accepted and widely used for cosmetic treatments. In this experiment, Vaseline is use for plug the gap so the air cant enter in to the respirometer. Eosin

Eosin is a red liquid that is usually used for biological experiments on "The speed of the required rate of O2 Insects and Plants". In use, eosin incorporated into the respirometer tube in order to see the speed the rate of oxygen, to test the speed of the insect or plant respiration when inserted into the tube where the air is limited. Simple Respirometer

Simple respirometer is a device that can be used to measure the speed of breathing some kind of living organisms such as insects, flowers, roots, fresh sprouts. If there is no significant change in temperature, respiratory rate can be expressed in ml / sec / g, the amount of oxygen being used by the trial every 1 gram of weight per second. Component Respirometer consists of two parts that can be separated, the tube specimen (where the animal or the plant under investigation) and the scale of the capillary tube up to 0.01 ml dikaliberasikan carefully. The second part could be put together very tightly and mounted to an air-tight at the fulcrum (basis) of wood or metal. The working principle This tool works on a principle that in there breathing oxygen used by organisms and there is carbon dioxide released by it. If breathing organism that is stored in an enclosed space and the carbon dioxide released by the organisms in a closed space is tied up, the air shrinkage will occur. Shrinkage velocity of air in the chamber can be recorded (observed) at the capillary scale.

Procedure
Tools and Materials 1. Simple respirometer 2. Injection 3. Ink 4. Cotton 5. NaOH (crystal) 6. Vaseline 7. Tweezers 8. Cricket Work Steps 1. Prepare tools and materials 2. Cover NaOH (crystal) with cotton and then put in to simple respirometer

3. Close bottle with finger for 3 minutes, after that put in ink to simple respirometer with injection. To prevent leakage, spread vaseline to the cover of respirometer

4. Observe the change of ink @3minutes between 9 minutes 5. Write the observation in observation table 6. Do this experiment in variations in different amount of cricket Picture

Entering the cricket

Close and wait until 3 minutes

Eosins injection

Observe the eosins movement

Result No. Amount of Cricket Positions Change of Eosin The 1st 3 minutes The 2nd 3 minutes 0.22 Scale Stop at 5.53 minutes Stop at 5.40 minutes The 3rd 3 minutes 0.19 Scale -

1. 1 Cricket 0.45 Scale 2. 2 Crickets 0.51 Scale 3. 3 Crickets 0.55 Scale *with total amount of time is 9 minutes Analysis

When there is 1 cricket in the respirometer, at the first 3 minutes the scale is showed at 0.45, showed 0.22 at second 3 minutes, and showed 0.19 at third 3 minutes. When the amount of cricket in respirometer changed to 2 crickets, at the first 3 minutes the scale is showed at 0.51, but in the second 3 minutes, the eosins move is stopped at time 5.53 minutes and no more move in third 3 minutes. And the last, when the amount of cricket in respirometer changed to 3 crickets, at the first 3 minutes the scale is showed at 0.55, but in the second 3 minutes, the eosins move is stopped at time 5.40 minutes and no more move in third 3 minutes.

Question
1. Make the graphic that show the relation between the amount of insect (cricket) and Oxygen needed!

Grafik hubungan antara jumlah serangga dengan kebutuhan oksigen

1 0.9 0.8 0.7 0.6 0.54 0.5 0.4 0.3 0.2 0.1 0 3 Menit pertama 3 Menit kedua 3 Menit ketiga 0.51 1 1

0.45

0.22

0.19

1 Serangga

2 Serangga

3 Serangga

2. Is it true that the change of the volume in the scale pipe is the amount of oxygen that required for bug in the certain time? Explain! - Yes it is. The change of volume in the scale pipe is show the amount of air volume in the enclosed space that effect of the respiration, oxygen is absorbed and the carbon dioxide is release and absorbs by the NaOH crystal. 3. What are the factors that influence the movement of liquid in the pipe of respirometer? - The amount of the insect in the respirometer and the amount of oxygen that absorbs by the insect.

4. What is the conclusion that we can make in this experiment! - Greater number of the insect that placed in the respirometer, the speed of the movement of the eosin in the pipe is faster (this condition show the amount of oxygen that used).

Conclusion
From the experiment, we can conclude: 1. The oxygen that required for the use of breathing by the animal is increased with the increasing number of the animal. 2. The factor that influences differences in the use of oxygen in the respirometer is the amount of animal(insect) that uses oxygen for respiration and the amount of oxygen that absorbs by the insect.

Bibliography
BUKU KREATIF BIOLOGI (BKS) KELAS XI SEMESTER GASAL. http://id.wikipedia.org/wiki/Respirasi http://id.wikipedia.org/wiki/Vaselin http://id.wikipedia.org/wiki/Eosin http://id.wikipedia.org/wiki/Respirometer http://2.bp.blogspot.com/_gbMjsXUlFM0/Snpfsms7WtI/AAAAAAAAAFA/mgphwiODhuI/s16 00-h/Slide31.JPG http://pharzone.com/materi%20kuliah/anfis%202/Sistem%20Respirasi.pdf http://www.sith.itb.ac.id/profile/pakAR/RESPIRASI.pdf