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Part #1
Questions and answers: 1- Calcenurin is involved in the activation of T cells 2- Calcenurin is inhibited by Cyclosporin 3- Rheumatoid factors are directed against Allotypic antibodies

Be sure to know the following. We had good questions about these topics but I dont remember them: 1- differences between primary and secondary immune responses. 2- The concept of bystander activation 3- markers of different cell types especially newly discovered cells like NK-T cells.

1) Which of the following lymphocytes has the highest concentration in blood: a- CD4+ CD8b- CD4- CD8+ c- CD4+ CD8+ d- CD4- CD82) Which of the following vaccines is administered orally: a-pollo-salk b- polio sabin c- smallpox d- hepatitis B e- a+b 3) Which of the following is not a part of the Ig gene superfamily a- Ig heavy chain b- T cell receptor a chain c- B cell receptor (signal transduction) d- B + C e- None of the above 4) Which of the following Igs has the lowest serum concentration : a- A b- G c- M d-D e- E

5) Which of the following Igs can cross the placenta: a- A b-G c-M d-B+C e- all of the above

6) Hyper IgM syndrome is due to a defect in: a- CD40L b- CD40 c- B cell receptor d- T cell receptor e- M chain 7) Which of the following is required in T cell maturatuin a- IL 1 b- IL 4 c- IL 5 d- IL 7 e- IL 9 8) Hereditary angloedema is due to deficiency of : a- C4 binding protein b- C1 inhibitor d- C4 e- None of the above Answers : 1-a 8-b 2-e 3-e 4-e 5-b c-C1

6-a

7-d

10) All of the following play a role in promoting the process of apoptosis except: bcl2 activation 11) A memory B cell has all of the following markers on its surface except: IgM (it has only the switched one IgG) 12) All the following cells has an antigen receptor on their surface except: Plasma cell (its function is only producing antibodies) 13) In screening for effective bone marrow transplantation with hematopoietic stem cell which of the following markers should be targeted as the cells can be identified by the FACS machine: CD34 14) Which of the following is the crucial step in the ADCC mechanism of cell killing? the presence of FC gamma receptor on the killer cell. 15) Which of the following immune mechanisms utilizes mannan binding lectin in their killing process? Complement system 16) In western Immunoblotting technique horseradish peroxidase (HRP) enzyme is bound to which of the following secondary antibody 17) Which of the following cells fail to home to secondary lymphoid organs because they lack homing adhesion molecules ? The memory cells ( they go to blood and tissue when ----- cells go to secondary lymphoid organs) 18) What is the best immune cenario of two different epitopes A and B, entering the body for the first time?

 Predetermined A specificity and predetermined B specificity alone (clonal selection theory, there is no receptor on B cell which has 2 specificities at the same time for the two epitopes) 1) Which of the following evasive mechanism is used by herpes viruses in order not to be detected by the immune system Interfere with the synthesis of class I MHC Ag inside the cell. 2) If an HLA- B1 cell is transplanted in an HLA-B5 fetus during the embryogenesis period, which of the following scenarios is going to happen During embryogenesis period there is a development of T-cell, So any cell is going to be recognized as foreign, and when we transplant B1 cell at embryo at the time of embryogenesis or development then it will be recognized as self, so the anti-B1cells in the thymus will be deleted and B1 will be accepted. 3) All of the following are considered as phagocytic cells except the natural killer cells 4) Which of the following Immunoglobulins faile to agglutinate specific antigenic determinant on the surface of cell IgM treated with a papaine enzyme 5) In the synthesis of monoclonal antibodies the hybredoma consist of Specific B cells fused with non specific myeloma cells 6) Which of the following Immunoglobulins has a receptor on the surface of NK cells IgG 7) In the primary immune response the gene rearrangement of the heavy chain starts with the binding of D to J 8) Somatic Hyper mutation involves the production of a receptor of high affinity this involves: the v region of the heavy and light chains and the region of the alpha and beta chain. 9) Super antigens: Bind to B chain of the T cell receptor and to class II MHC 27) A new born baby has class I MHC haptotype as : A1A3 B5B10 C1C9, born to a mother who has a haptotype of A2A3 B7B10 C4C9, which of the following haptotypes could be the father of this baby? A5A1, B5B8, C1C5 ( the baby inherit one from the father and one from the mother, it is a codominant type of inheritance {i.e. the baby has A1A3 then A3 from the mother and A1 from the father} 28) Restriction in Ag presentation involves: T cell receptor recognises non-self Ag in association with self MHC. 29) Polymorphism among the MHC allelic forms involves the structural area of : The a1,a2 domains in class I (the area of Ag presentation) 30) During the secondary immune response class switching involves the formation of any of these immunoglobulins except: IgM, IgD

31) I TAMS (immune receptor Tyrosin- based activation motif) are present in all of the following chains except T-cell receptor (the B-chain of T-cell receptor) 32) CD19 : - Is present on naieve B cell - Could not be recognized in any T-cell subset - Can be targeted as B-cell marker for the FACS machine all of the above

19) All of the following proteins are part of the (ptk) enzyme system involved in the signaling of T-cell through I TAMS phosphorylation except: bcl2 20) The cytoplasmic protein needed for the formation of a complex with cyclosporine a in order for the drug to work by finally inhibiting the formation of IL2 in T-helper cell is: The immunophilines (it bind to cyclosporine a to inhibit calcenurin from working)

21) Which of the following lymphokine receptor is defective in patient with severe combined immunodeficiency syndrome: IL7, IL2, IL4 22) A memory cell has all the following markers on its surface except: IgM 23) Oral tolerance is mainly mediated by being exposed to large oral dose of an antigen, which of the following lymphocytes is involved in this process: Naieve T-vell from gamma,delta type 24) Which of the following is or are involved in receptor editing in order not to bind self Ag and form an autoimmune disease? the variable light genes in T-cell receptor 25) In negative selection of T-cells in thymus gland occurs during cell development of which of the following stages the double positive stage 26) All of the following play a role in apoptosis except: Bcl2 activation (repeated question)

MATCHING: -Severe combined immuneodificiency disease RAG1 and RAG2 - Hyper IgM syndrome CD154 - Digeorge syndrome Tetany

- Multiple sclerosis increased TH1 Activity - Toxic shock syndrome Super antigen - CD80 CD28 - CD40 CD154 - ICAM LFA1 - LCK CD4

TRUE AND FALSE 1) If an antibody is cross reactive with an antigen, it implies that it does have a lower sensitivity compared to an antibody that is not cross reactive. False (cuz sensitivity will not be affected by cross reaction, but the specificity will be lower, because we are talking about false positive) 2) Diversity in the light chain (lambda) is 4 times more than that lf (kappa) chain because we have 4 different types of lambda False (the constant region has nothing to do with diversity) 3) Altered peptide ligand vaccine, although it sometimes could lead to receptor activation but it provides suppression of T-helper cells instead of proliferation and differentiation TRUE 4) If the thymus gland is removed immediately after birth, Then the baby's response to viral infection will be impaired. False (nothing will occur after removing the thymus gland unless we ------- the baby) 5) If TH2 is diffected then Isotype switching IgE will be affected. True ( because TH2 provide B cell with IL2,I4,I5 responsible for isotype switching) 6) Allelic exclusion phenomena implies the expression of one allotype on immunoglobulin and T cell Bchain only.