Microbiology Practical I

The following slides were contributed by your fellow BIO 251 students
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Lab 1: Eukaryotes and Microscopy

Know the parts of the microscope

Lab 1: Eukaryotes and Microscopy

Paramecium caudatum

Lab 1: Eukaryotes and Microscopy

Euglena gracilis

Lab 1: Eukaryotes and Microscopy

Amoeba proteus

Lab 1: Eukaryotes and Microscopy

Fungus Saccharomyces cerevisiae

Lab 1: Eukaryotes and Microscopy

Cyanobacteria ~ Nostoc

~Bacteria slides~
Micrococcus luteus

~Bacteria Slides~
Bacillus subtilis

~Bacteria Slides~
Bacillus anthracis

~Bacteria Slides~
Rhodospirillum rubrum
Sorry not in focus

~Bacteria Slides~
See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining [ Adobe Acrobat of Gram and Endospore ]
Some Endospore Staining ~

Streak Plate:

Know colony characteristics (color, elevation, margin, etc.)

Spread Plate vs. Streak Plate:

Streak Plate:

Spread Plate:
Mixed Bacillus subtilis & Serratia marcescens

Practical I Unknown Tests:

~ Simple Stain ~ Gram Stain ~ Endospore Stain ~ Acid-Fast Stain (Demo) ~ Capsule Stain (Demo) ~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep) ~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles
(Gas Pak, Eugon Agar, Thioglycollate Broth)

Gas Pak Anaerobic vs. Aerobic:

Dilution Problems:

Dilution Problems:

Bacterial Growth Curve:

Make sure you know how to:

Use a spectrophotometer to measure absorbance.

Produce a log graph of the calculations.

Calculate generation time from a growth curve.