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Contents
Introduction Development of the Junctional epithelium Structure of the Junctional epithelium Epithelial attachment apparatus Turnover of the Junctional epithelial cells Dynamic aspects of the junctional epithelium Expression of various molecules and their functions Junctional epithelium adjacent to oral implants Regeneration of the Junctional Epithelium Role of junctional epithelium in the initiation of pocket formation. Long junctional epihelium
Gingival Epithelium
The gingival epithelium is similar to epidermis in that it shows a distinct sex difference.
In the female, a large Feulgen-positive particle has been found adjacent to the nuclear membrane ; in the males , a similar but smaller particle is present in 1 to 2 % of the cells.
Marwah, A.S., and wienmann : A sex difference in epithelial cells of human gingiva. J. Periodont,26:11,1955.
As the tooth erupts, the superficial portion of the attachment progressively separates from the enamel, leaving a cuticle adherent to the tooth surface and forming a V-shaped space- the Gingival sulcus
Gottlieb
Weski, Gross,Euler and wodehouse contended that the gingival sulcus is formed by a split in the Epithelial attachment rather than by separation from the tooth.
Wearhaug
Orban
Gottlieb
Stern
Dento-gingival junction
Schroeder and Listergarten first clarified the anatomy and histology of the dentogingival junction in their monographFine structure of developing epithelial attachment of human teeth
JE
CT
Enamel space
Coronally: 15-30 cells thick. Apically: narrows to 1-3 cells Length: 0.25 1.35mm Stratified squamous non-keratinized epithelium that is made up of two strata only i.e., a basal layer(stratum basale) and a suprabasal layer(stratum suprabasale)
Cytokeratins in JE
JE expresses simple keratin markers such as K8 and K4 which demarcates the SE from JE. JE does not express K1 & K10 K19 is present in all layers of JE in monomeric state which is expressed more in proliferative cell layers JE attached to tooth through the K5 and 14hemidesmosomes assembly K5,6,7,and 9 are typical of that of simple,rapidly proliferting,less differentiated epithelia.
Non-keratinzed
Junctional epithelium
Junctional epithelium is unique as it possess 2 basement membranes the internal and external basal lamina
Remarcable permeability
Junctional epithelium
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Schroeder 1969,1981 Schroeder and listgarten 1977 Yamasaki et al 1979 Schroeder and Munzel-ppedrazzoli 1970
The junctional epithelium, particularly its basal cell layers, is well innervated by sensory nerve fibers
The epithelium- tooth interface is a unique structure wherein epithelial cells adhere by means of bonafide hemidesmosomes to an epithelium derived extracellular matrix lacking most of the common BM components. Moreover, TF cells differ from connective tissue facing cells and their production of extracellular matrix, but also by their cytoskeletal architecture.
Marketta Horrnia et al: The dento- epithelial junction:cell adhesion by Type 1 hemidesmosomes in the absence of a true basal lamina. J Periodontol 2001;72:788-797
DAT cells
Salonen coined the term DAT cells.(1989) Innermost suprabasal cells. They form and maintain the internal basal lamina that faces the tooth surface
DAT cells possess stress fibers arranged in parallel to the tooth axis and to the presumable cervical-line in the cytoplasm, and microvilli-like structures on their enamel surfaces.
J Periodontal Res. 2005 Aug;40(4):354-63.
Cytoskeleton and surface structures of cells directly attached to the tooth in the rat junctional epithelium.
Ishikawa H, Hashimoto S, Tanno M, Ishikawa T, Tanaka T, Shimono M.
DYNAMIC
Hemidesmosomes
a structure representing half of a desmosome, found on the basal surface of some epithelial cells, forming the site of attachment between the basal surface of the cell and the basement membrane. Extracellular protiens- laminin-5 Intracellular protiens- plectin and BP
Hemidesmosomes are transmembrane cell-matrix junctional complexes that are intracellularly connected to the cytoskeleton filaments of epithelial cells
Laminin
Laminin-332
At the coronal part of the JE, the DAT cells typically express a high density of transferrin receptors,which supports the idea of their active metabolism and high turnover
JE consists of active population of cells and antimicrobial functions which together form the 1st line of defense against microbial invasion into tissue
Several antimicrobial mechanisms exist in the junctional epithelium. In the coronal part of the junctional epithelium quick cell exfoliation (1) because of rapid cell division (2) and funnelling of junctional epithelial cells towards the sulcus hinder bacterial colonization. Laterally, the (external) basement membrane forms an effective barrier against invading microbes (3). Active antimicrobial substances are produced in junctional epithelial cells. These include defensins and lysosomal enzymes (4). Epithelial cells activated by microbial substances secrete chemokines, e.g. interleukin- 8 and cytokines, e.g. interleukins -1 and -6, and tumour necrosis factor-a that attract and activate professional defense cells, such as lymphocytes (LC) and polymorphonuclear leukocytes (PMN). Their secreted product, in turn, cause further activation of the junctional epithelial cells (5).
Location and functions of molecular factors associated with the junctional epithelium
Bacterial agents -endotoxins,hydrogen sulfide, butyric and propionic acids.bacterial collagenases and variety of enzymes,such as hyaluronidases and neuraminidases.
Host derived agents - complement factors,prostaglandins, different cytokines, intracellular enzymes, and products of tissue breakdown such as lactate dehydrogenase, aspartate aminotranfearases and collagen peptides.
The degeneration and detachment of DAT cells exposes the tooth surface and creates a subgingival niche suitable for the colonization of anareobic gram ve bacteria and apical growth of dental plaque
Antimicrobial agents and leukocyte-derived enzymes such as lysozyme, alkaline phosphatase,-glucoronidase, cathepsin D, elastin ,collagenase and lactoferrin.
Secondary granules
lactoferrin
It has high affinity for iron and it acts on bacteria by causing iron depletion, and thus reduction in bacterial cell division rate, glucose metabolism and macromaolecular synthesis
health
High conc of lactoferrin hamper epithelial cell growth by interfering with their adhesion and spreading .The molecule may have a role in delaying the repair of the JE/DAT cell population during severe inflamation.
disease
600g/ml
1500g/ml
A functional epithelial seal must be re-established at the most coronal portion of the tissues and be no more than 2 mm in length.
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The long junctional epithelium is formed by the keratinocytes of the oral epithelium repopulating the wound and migrating over the root surface. Fragments of fibronectin are thought to be chemotactic to various periodontal subtypes and are thought to play an important role in the formation of Long Junctional Epithelium
The formation and organization of the wound clot over the root surface is a property of the fibronectin molecules that are expressed in the early phases of wound healing. The oral keratinocytes express integrins such as 51 that are normally present in the epithelium when they come into contact with fibronectin. These integrins promote the migration of these keratinocytes over the root surface and form the long junctional epithelium.
curettage
Chemical agents
Surgical techniques
Glickman and Prichard have advocated performing a gingivectomy to the crest of the alveolar bone and debriding the defect
because
1st approach
RST
2nd approach
REFERENCES
Clinical periodontology- IRVING GLICKMAN : 3rd edition ORBAN,s Oral histology and Embryology- 11th edition
CLINICAL PERIODONTOLOGY CARRANZAS- 10TH EDITION
Marja T,Jukka I.Salonen:structure and function of the toothepithelial interface in health and disease. Periodontology 2000,vol 31,2003.
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