Effects of pH, temperature, enzyme concentration, and substrate concentration on enzyme activity.

4S1- 15th April 2012

Learning Outcomes
explain the effects of pH, temperature, enzyme concentration, and substrate concentration on enzyme activity. relate the mechanism of enzyme action with pH, temperature, enzyme concentration and substrate concentration,

Foamy Vengeance!!!

Revision?
What is enzyme? (Group Azwin) Name 8 characteristic of an enzymes? (Group Nisa) What is enzymes substrates complex? (Group Hazwani) What is active site? (Group Amirah) How to name an enzymes? (Group Sarah) What is intracellular and extra cellular enzymes? (Group Juhaliza)

Temperature
A : At low temperature enzyme catalyzed reaction is slow

Temperature
B: As temperature increase Kinetic energy increase Enzyme substrates collision increase More ES complex formed

Temperature
C:
The optimum temperature is the temperature where an enzyme catalyes a maximum reaction Causes rapid movement and collision bet the Substrate and enzyme

Temperature
D: At high temperature Enzyme catalyzed reaction will stop Bond maintaining the structure of the enzyme start to break and active site lose it shape Enzyme is denature

Denatured ?
Take away or alter the natural qualities of.

Denatured

pH
Extremely high or low pH values generally result in complete loss of activity for most enzymes. As with activity, for each enzyme there is also a region of pH optimal stability. The optimum pH value will vary greatly from one enzyme to another

But there is certain enzyme working in diff pH

Low pH
ES-complex does not form Active site repel substrate because to much H+ ions Because the condition to acidic

High pH
ES-complex does not form Active site repel substrate because to much OHions Because the condition to alkaline

Neutral pH
ES-complex does form Active site binds with substrate because the condition is suitable

Analogi Tol
Enzyme adalah Tol Substrate adalah Kereta

Problem 1
10 toll opened 100 cars What happened? If 1 toll can managed 2 car per minutes ? How many minutes to managed 100 cars?

Problem 2
10 toll gate opened 50 cars If 1 toll gate can managed 2 car per minutes ? How many minutes to managed 50 cars?

Problem 3
8 tollgate opened 100 cars If 1 toll gate can managed 2 car per minutes ? How many minutes to managed 100 cars?

Problem 4
4 tollgate opened 100 cars If 1 toll gate can managed 2 car per minutes ? How many minutes to managed 100 cars?

Substrate concentration
Red 0.2% When substrate increase, ES reaction increase. More substrate will be collide with the enzyme The high concentration of substrate will rise the reaction to maximum rate until not enough enzyme supply

Substrate concentration
Green 0.1% The concentration of enzyme become limiting factor to consentation of substrate The rate of reaction is lower than 0.2%

Enzyme concentrations
Concentration of enzyme increase, more enzyme are available Maximum rate is reached due to limited amount of substrate

Enzyme concentrations
As more active site are available More substrate can be converted into products Double enzyme cons =double rate of reactions

Apple?