ON-THE JOB TRAINING

Objectives
1.

Familiarization in using laboratory instruments Application of knowledge learned inside the classroom Broaden knowledge and experience

2.

3.

STUDIES ASSISTED
Improvement of Brown Rice Quality FFA Lipase Activity Antioxidant Potential of Rice Field Algae as a Source of Biodiesel Extraction using hexane Extraction using Pet. Ether Grain Quality Evaluation of NCT Rice Samples Physicochemical Analyses -Moisture Content Determination -Crude Protein -Amylose

BROWN RICE

Paddy Rice Hull = Brown Rice

(Palay Ipa = Pinawa)
Whole Rice

Brown Rice
Advantages
Disadvantages

 Light

polishing of grain for <3 sec can significantly improve the cooking and eating quality of brown rice (Tamang et al, 2011) Light polished brown rice is more susceptible to rancidity. Light polishing of grain exposes surface oil. Thus, hydrolysis of oil immediately occurs

Oleic Acid

Linoleic Acid

Sample

Extraction

Color Development

Reading

RESULTS

Treatments
Unpolished Polished 5 mins

Nylon
0.052509 0.191791 0.138491 0.106773 0.082474

Orocan
0.047019 0.344478 0.161359 0.162527 0.153152

Box
0.064513 0.267463 0.342901 0.244449 0.245442

10 mins
15 mins

Sample

Extraction using buffer

Incubation

Extraction

Color Development

Reading

RESULTS

Treatments
Unpolished Polished 5 mins 10 mins 15 mins

Nylon
0.0383 0.04881 0.03607 0.03133 0.02682

Orocan
0.03836 0.05004 0.0335 0.0306 0.02975

Box
0.0388 0.03733 0.02688 0.02357 0.02846

Sample

Extraction

Color Development

Reading

RESULTS Treatments
Unpolished Polished 5 mins 10 mins 15 mins

Nylon
28.367
21.268 19.976 22.228 19.257

Orocan
27.599
22.355 26.353 21.957 22.183

Box
23.037
17.648 20.615 19.533 17.983

ALGAE
Unicellular

plant-like organism which contain chlorophyll pigments.

ADVANTAGES: They belong in the third generation feedstock High energy content Grow fast anywhere

BIODIESEL
-monoalkyl esters of long chain fatty acids derived
from a renewable lipid feedstock, such as vegetable oil or animal fat.

Sample collection

Washing and Drying

Cutting and Extraction of Oil

RESULTS
Sample
1 2 3 4

% Oil
7.330668 9.213412 6.435232 10.39997

PET ETHER EXTRACTION

Sample collection

Washing and Drying

Milling and Extraction of Oil

RESULTS
Sample
1 2 3 4 5

Average Moisture Content

93.9057 95.33013 94.56167 97.46981 93.69072

% Oil from Soxhlet Extraction

2.011469 1.576451 1.130777 1.526568 ---------------

GRAIN QUALITY EVALUATION

PHYSICOCHEMICAL CHEMICAL PROPERTIES
-

Determines the cooking, eating and processing quality of rice samples. *Amylose Content ( 20-25%) *Moisture content (12-14%) *Crude Protein

AMYLOSE CONTENT
The sample is dispersed in alkali solution and subjected to iodine colorimetry.

Classification

Apparent Amylose Content

Waxy/Glutinous
Very Low Low Intermediate

2%
2.1%-10% 10.1%-20% 20.1%-25%

High

More than 25%

MOISTURE CONTENT DETERMINATION

Water is removed from sample by volatilization through heating to the required temp. Weight loss determines the moisture content of the sample. Range: 12%- 14%

CRUDE PROTEIN DETERMINATION

-this is based on Kjeldahl method.
catalyst Sample + H2SO4 CO2 +H2O + (NH4)2SO4

RESULTS
Moisture Content
Sample A IL-05 IL-06 IL-07 IL-08 IL-09 7.4 7.3 10.3 11.3 11.8 MC B 7.2 7.2 10.2 11.5 11.7 AVE %MC Sample A UAT-16 7.3 7.3 10.3 11.4 11.8 UAT-17 UAT-18 UAT-19 UAT-20 UAT-21 6.61 6.94 7.29 6.85 6.73 6.64

Crude Protein
% CP B 6.71 6.71 7.28 6.97 6.80 6.69 AVE %CP 6.7 6.8 7.3 6.9 6.8 6.7

Amylose
AVE % Class Amylose 25.3 23.6 19.5 20.9 21.6 21.3 H I I I I I

IL-10
IL-11

11.9
12.0

12.0
12.2

12.0
12.1

UAT-22
UAT-23

6.86
7.08

6.94
7.14

6.9
7.1

19.9
5.8

I
VL

THANK YOU PO !
PREPARED BY: KRISTINA S. VILLADULID GIRLIE EUNICE P. LOPEZ