COMPARATIVE STUDY OF SWETA PARPATI &

SHEETAL PARPATI IN MUTRAKRICHRA W.S.R

TO THEIR ANTI MICROBIAL STUDY
- BY
DR. NITU SINGH
P.G SCHOLAR
DEPARTMENT OF RASA SHASTRA

Dr R S Hiremath. MD Dr P G Jadar. MD
Dept of Rasashastra Dept of Rasashastra

K.L.E’s Shri B M K Ayurveda Mahavidyalaya. Belgaum, Karnataka.

 NEED FOR STUDY

• There are many formulations told for

mutrakrichra in Ayurveda. Shweta parpati and
sheetal parpati are two such preparations
• It is usually prepared from kajjali or by
adding some ingredients to kajjali.

• Sweta parpati is nirgandha, and sheetal

parpati is sagandha preparation .
 OBJECTIVES
• Physical and chemical analysis of raw
materials
• Shodhana of ingredients and preparation of
Shweta parpati and sheetal parpati.
• Analytical study of prepared compounds&
Comparative evaluation of antimicrobial
activity of both formulations on -
1) E.coli
2) Proteus mirabilis
3) Staphylococcus aureus
4) Candida albicans
 MATERIALS & METHODS
 Raw materials were purchased and authentificated.
 Kalmisora nirmalikarana.
 Preparation of ela hima.
 Kalmisora shodhana -- in order to enhance its diuretic
property.
Kankshi shodhana.

 Navsadara shodhana.

Preparation of triphala kwatha.

Gandhaka shodhana.
Preparation of shweta parpati:

Kalmi sora-96 gm

shodhita kankshi-12 gm

Shodhita navasadar-6gm

Preparation of sheetal parpati:

Kalmi sora-128gm

Shodhita gandhaka-2 gm
 ANALYTICAL PROCEDURES

QUALITATIVE ANALYSIS

Basic radicals : Na+, K+, NH4+, Al+3, Zn+2, Ba+2, Ca+2 ,

Mg+2

Acid radicals: Cl- , Br-, So4, No3 may be present in the

compounds.

Determination of pH values-

It is noted about 4.19 in shweta parpati & 6.76 in sheetal

parpati. by help of ph meter
 Result
Organoleptic Characteristics:
Material Before Shodhana After Shodhana

SORA Pandura Sikatakara Pandura Small

Odourless Karpurabha crystals
Smell of Ela Opaque
KANKSHI Yellowish White; Transparent Clear White ; Amorphous
Hard Crystals ; Guru,Snigdha Soft powder ; Laghu, Ruksha

NAVASADAR White White

Crystalline Crystalline
Odourless Odourless

GANDHAKA Greenish yellow Yellow

Snigdha Snigdha
Hard crystals Brittle Crystals
Contd…
Shweta parpati: Sheetal parpati:
• Colour: white • Colour: light brown
• Odour: Kshariya • Odour: smell of gandhaka
• Touch: smooth • Touch: rough
• Taste: lavana,sheeta • Taste: lavana,sheeta
• pH: 4.19 • pH: 6.76
• Total ash: 132.8451% • Total ash: 99.1011%
• Total chloride: 3.1475% • Total sulphates: 0.2141%
• Total sulphate: 4.0824% • Qualitative test for K+ &
• Qualitative test for Fe+3: Positive
K+&Al3+: Positive
 Antimicrobial Activity
E. coli S. aureus Pr. mirabilis C. albicans
%
conc B A B A A B
A B
1% +VE +VE +VE +VE -VE +VE +VE +VE

2.5% +VE +VE -VE -VE -VE +VE +VE +VE

5% -VE -VE -VE -VE -VE -VE -VE +VE

7.5% -VE -VE -VE -VE -VE -VE -VE +VE

-VE -VE -VE -VE -VE

10% -VE -VE -VE
 Colony morphology for identification of
micro organisms
1. S.aureus form large colonies of 2-4 mm dia,yellowish
Circular clusters

• E.coli form large ,thick grayish colonies.

3. P.mirabilis show swarming motility& negative to lactose.

9. C.albicans are yeast like fungus .

5. Agar diffusion method-None of dilution show zone of inhi

bition for any organisms.

6. Agar dilution method-A: Shweta parpati ,B: Sheetal parpati

Growth indicated by +VE
No growth indicated by -VE
 CONCLUSION
1.The pH of both parpatis fall in range that is required for bacterial
growth. So it can be said that action of parpatis are independent of change
in pH.

2.The two parpatis show encouraging antimicrobial activities at different

concentrations in different strains of bacteria.

3. Antibiotic susceptibility tests are not guarantee that an Anti microbial

activity will be effective or ineffective

4.The general method of diagnosis in ayurveda is based on doshas like

vata ,pitta and kapha.So ayurvedic formulations have different role in
every human being.

5.This study gives a scope for in vivo studies regarding action of these
formulations in reducing puss and albumin.
THANK U