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6th. Lab
Erythrocyte
.Sedimentation Rate
.Bleeding time
.Clotting time dlerqader74@yahoo.com
Lab. no. 6: Erythrocyte Sedimentation Rate
(ESR)
(Westergren method)
Procedure:
Under aseptic conditions withdraw (1.6 ml ) of blood
from a vein and mix with (0.4 ml) of 3.8% sodium
citrate as anticoagulant (4:1), suck this blood into a
Westergren tube, adjust the top level at (0 mm)
mark. Wipe the blood off its tip and set the tube in a
vertical position in the Westergren rack. RBCs settle
down leaving a column of clear plasma on the top.
After exact one hour read the upper level of the RBC
column. The result is expressed in mm/1st hr.
hr
dlerqader74@yahoo.com
Lab. no. 6: (ESR) (Westergren method) Cont…..
Procedure:
• Clean a finger ball with spirit and with a
sterile lancet give at least 2 mm deep
prick. Note the time.
• Every 30 sec. blot off the drop of blood
by a filter paper until no more blood
appears on the puncture site. Note the
time at which the bleeding has stopped.
dlerqader74@yahoo.com
Lab. no. 6: Bleeding time (illustration) Cont…..
.Every 30 sec
no. of blots
B.T = = ? min.
2
dlerqader74@yahoo.com
Lab. no. 6: Bleeding time (Dukeś method) Cont…..
Procedure:
6.Clean the finger with spirit and give a deep prick
with a sterile lancet, squeeze the finger to obtain
a large drop of blood. Note the time (T1).
7.Fill in two plain capillary tubes with blood.
8.After (2 min.), start breaking small pieces of one
capillary tube every 30 sec. until a fibrin thread
is seen in the interval between the 2 broken
equivocal ends. Note the time (T2).
9.The interval between T1 and T2 is the clotting
time.
dlerqader74@yahoo.com
Lab.no.6: Clotting time (illustration)
Fibrin thread
T2
1 2 3 4 5 6 7 8