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mendapatkan pengurangan nilai total sebesar 10%, lebih dari 1 kali mendapatkan pengurangan nilai total sebesar 30% Kehadiran 100% praktikum. Jika berhalangan karena sakit (hanya 1 kali pertemuan) dan tidak ada waktu susulan, dipersilakan mempersiapkan semua keperluan praktikum sendiri. Kehadiran di kelas paling lambat 5 menit, lewat dari waktu tersebut tidak diperkenankan masuk ke dalam kelas Nilai tugas 20% dari keseluruhan nilai teori UTS 40 % dan UAS 40% Nilai teori 60% dan praktikum 40% menjadi nilai akhir
Materi
Sel prokariot dan sel eukariot Bakteri Jamur Virus Protozoa Presentasi tugas in english 1 UTS
Tina Rostinawati
Pendahuluan
Sel adalah unit terkecil yg menunjukkan
semua sifat yg dihubungkan dgn kehidupan Semua sel mengandung makromolekul ttt : lipid, protein, asam nukleat, polisakarida Berdasarkan komposisi dan organisasinya sel dibagi menjadi sel prokariot dan eukariot
Cell Theory
All organisms are composed of one or
more cells. Cells are the smallest living units of all living organisms. Cells arise only by division of a previously existing cell.
Prokaryotic Cells
Simplest organisms
Cytoplasm is surrounded by plasma membrane and encased in a rigid cell wall composed of peptidoglycan. no distinct interior compartments
gram-positive thick single layer wall that retains a violet dye from Gram stain procedure gram-negative multilayered wall does not retain dye Susceptibility of bacteria to antibiotics depends on cell wall structure.
Cell Wall
Rigid peptidoglycan polysaccharide coat that gives the cell shape and surround the cytoplasmic membrane. Offers protection from environment.
Http://micro.magnet.fsu.edu/cells/bacteriacell.html
Plasma Membrane
Layer of phospholipids and proteins that separates cytoplasm from external environment. Regulates flow of material in and out of cell.
Http://micro.magnet.fsu.edu/cells/bacteriacell.html
Plasma Membrane
Phospholipid bi-layer
that separates the cell from its environment. Selectively permeable to allow substances to pass into and out of the cell.
Http:micro.magnet.fsu.edu/cells/animal/plasmame mbrane.html
Plasma membrane
Cytoplasm
Also known as protoplasm is location of growth, metabolism, and replication. Is a gel-like matrix of water, enzymes, nutrients, wastes, and gases and contains cell structures.
Http://micro.magnet.fsu.edu/cells/bacteriacell.html
Ribosomes
Translate the genetic
Http://micro.magnet.fsu.edu/cells/bacteriacell.html
Mesosome
Infolding of cell
membrane. Possible role in cell division. Increases surface area. Photosynthetic pigments or respiratory chains here.
Http://www.med.sc.edu:85/fox/protobact.jpg
Nucleoid
Region of the
cytoplasm where chromosomal DNA is located. Usually a singular, circular chromosome. Smaller circles of DNA called plasmids are also located in cytoplasm.
Http://micro.magnet.fsu.edu/cells/bacteriacell.html
Nucleus Contains most of the heriditary material (DNA) of the cell Plasmids extra-chromosomal DNA usually present in multiple copies often code for pathogenesis factors and antibiotic resistance factors involved in bacterial replication. Ribosome Site of protein synthesis Flagella locomotory organelles chemotaxis
Pili synonym: fimbriae types of pili varies in sexual conjugation allow adhesion to host epithelial surfaces in infection
Endospores (spores) a dormant form produced by certain bacteria when starved vegetative form resistant to adverse conditions spore cytoplasm is dehydrated and contains calcium dipicolinate which is involved in the heat resistance of the spore are commonly found in the genera Bacillus and Clostridium.
Eukaryotic Cells
Characterized by compartmentalization by
an endomembrane system, and the presence of membrane-bound organelles.
Central vacuole plants, storage Vesicles (smaller) Chromosomes - DNA and protein Cytoskeleton (internal protein scaffolding) Cell walls plants and fungi
Repository for genetic material Directs activities of the cell Usually single, some cells several Surface of nucleus bound by two phospholipid
bilayer membranes
nuclear membrane Nuclear pores protein gatekeepers Usually proteins going in and RNA going out Nucleolus - region of intensive ribosomal RNA synthesis
Nucleus
Nucleus
Chromosomes
DNA of eukaryotes is divided into linear
chromosomes.
exist as strands of chromatin, except during cell division associated with packaging histones, packaging proteins nucleosomes
Endosymbiotic hypothesis
Ribosomes
Translate the genetic
code into proteins. Found attached to the Rough endoplasmic reticulum or free in the cytoplasm. 60% RNA and 40% protein.
Http://micro.magnet.fsu.edu/cells/animals/ribosom es.html
EUKARYOTIC RIBOSOMES
small subunit: 40S One rRNA molecule (18S) 33 different proteins, designated S1-S33
large subunit: 60S Three rRNA molecules (5S, 5.8S, and 28S) 50 different proteins, designated L1-L50
Type 18S 5S 5.8S 28S Approximate number of nucleotides 1,900 120 156 4,700 Subunit location 40S 60S 60S 60S
Endomembrane System
Http://micro.magnet.fsu.edu/cells/animals/endoplasmicreticulum.html
Golgi Apparatus
Modifies proteins and
lipids made by the ER and prepares them for export from the cell. Encloses digestive enyzymes into membranes to form lysosomes.
Http://micro.magnet.fsu.edu/cells/animals/golgiapp aratus.html
Endomembrane System
Golgi apparatus
collection of Golgi bodies collect, package, and distribute molecules synthesized at one
location in the cell and utilized at another location Front - cis , Back trans Cisternae stacked membrane folds
Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Protein
Vesicle Migrating budding transport from rough vesicle endoplasmic reticulum Ribosome
Lysosome
Single membrane
bound structure. Contains digestive enzymes that break down cellular waste and debris and nutrients for use by the cell.
Http://micro.magnet.fsu.edu/cells/animals/lysosom e/html
Copyright The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
Cytoplasm
Phagocytosis
Endoplasmic reticulum
Food vesicle
Golgi apparatus
Lysosomes
Plasma membrane
Extracellular fluid
Chloroplasts
have enclosed internal compartments of stacked grana, containing thylakoids found in photosynthetic organisms
Mitochondria
A. "Powerhouse of the cell" - cellular metabolism B. Structure- outer and inner membranes, cristae C. Have their own DNA
Chloroplasts are larger and more complex than mitochondria Grana closed compartments of stacked membranes Thylakoids disc shaped structure light capturing pigment Stroma fluid matrix
Chloroplasts
Plant Cells
Central vacuole
Cell wall
often found in the center of a plant, and serves as a storage facility for water and other materials primary walls laid down while cell is growing middle lamella glues cells together secondary walls inside the primary cell walls after growth
Cytoskeleton
Plant Cell
Animal Cells
Phylogeny of bacteria
A prokaryotic species is a collection of strains that share many stable properties and differ significantly from other groups or strains. A species (genomospecies) is a collection of strains that have a similar G+C composition and 70% or greater similarity as judged by DNA hybridization experiments.