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DiagnosticTesting
Phases of Diagnostic Testing
Pretest Phase Preparing the client Knowing about the test ordered Gathering equipment and supplies
Intratest Phase
Collecting the specimen Performing or assisting Providing emotional and physical support Monitoring Correct labeling, storage, and transportation of specimen
A CBC helps your health professional check any symptoms, such as weakness, fatigue, or bruising, you may have. A CBC also helps him or her diagnose conditions, such as anemia, infection, and many other disorders.
The numbers of each one of these types of white blood cells give important information about the immune system. Too many or too few of the different types of white blood cells can help find an infection, an allergic or toxic reaction to medicines or chemicals, and many conditions, such as leukemia.
If the RBC count is low (anemia), the body may not be getting the oxygen it needs. If the count is too high (a condition called polycythemia), there is a chance that the red blood cells will clump together and block tiny blood vessels (capillaries). This also makes it hard for your red blood cells to carry oxygen.
Hemoglobin (Hgb).
Hemoglobin is the protein molecule within red blood cells that carries oxygen and gives blood its red color. The hemoglobin test measures the amount of hemoglobin in blood and is a good measure of the blood's ability to carry oxygen throughout the body.
The MCV is the average volume of a red blood cell. This is a calculated value derived from the hematocrit and red cell count. Normal range may fall between 80 to 100 femtoliters (a fraction of one millionth of a liter). The MCH value is the amount of hemoglobin in an average red blood cell. This is a calculated value derived from the measurement of hemoglobin and the red cell count. Normal range is 27 to 32 picograms.
The MCHC measures the concentration of hemoglobin in an average red blood cell. These numbers help in the diagnosis of different types of anemia. This is a calculated volume derived from the hemoglobin measurement and the hematocrit. Normal range is 32% to 36%.
Red Cell Distribution Width (RDW) is a measurement of the variability of red cell size and shape. Higher numbers indicate greater variation in size. Normal range is 11 to 15.
If there are too few platelets thrombocytopenia) , uncontrolled bleeding may be a problem. If there are too many plateletsthrombocytosis) , there is a chance of a blood clot forming in a blood vessel. Also, platelets may be involved in hardening of the arteries (atherosclerosis).
NORMAL VALUES
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Pregnant women: 1st trimester: 6,60014,100/mcL or 6.6 14.1 x 109/L 2nd trimester: 6,90017,100/mcL or 6.9 17.1 x 109/L 3rd trimester: 5,90014,700/mcL or 5.9 14.7 x 109/L Postpartum: 9,70025,700/mcL or 9.7 9/L 25.7 x 10 6/23/2013 copyright (your organization) 2003
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Hematocrit (HCT)
Men: Pregnant women: 42%52% or 0.42 0.52 volume 1st trimester: fraction (SI units) 35%46% Women: 2nd trimester: 30%42% 37%47% or 0.37 0.47 volume 3rd trimester: fraction 34%44% Children: Postpartum: 30%44% 32%44% Newborns: copyright (your organization) 2003 44%64%
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Hemoglobin (Hgb)
Men: 1418 grams per deciliter (g/dL) or 8.711.2 millimoles per liter (mmol/L) (SI units) Women: 1216 g/dL or 7.4 9.9 mmol/L Pregnant women: 1st trimester: 11.4 15.0 g/dL or 7.1 9.3 mmol/L
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2nd trimester: 10.014.3 g/dL or 6.28.9 mmol/L 3rd trimester: 10.2 14.4 g/dL or 6.3 8.9 mmol/L Postpartum: 10.4 18.0 g/dL or 6.4 9.3 mmol/L Children: 9.515.5 g/dL Newborn: 1424 g/dL
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Electrolytes are measured by a process known as potentiometry. This method measures the voltage that develops between the inner and outer surfaces of an ion selective electrode. Electrolyte tests are performed on whole blood, plasma, or serum, usually collected from a vein or capillary.
Sodium
Sodium plays a major role in regulating the amount of water in the body. Also, the passage of sodium in and out of cells is necessary for many body functions, like transmitting electrical signals in the brain and in the muscles. The sodium levels are measured to detect whether there's the right balance of sodium and liquid in the blood to carry out those functions.
If a child becomes dehydrated because of vomiting, diarrhea, or inadequate fluid intake, the sodium levels can be abnormally high or low, which can cause a child to feel confused, weak, and lethargic, and even to have seizures.
Potassium
Potassium is essential to regulate how the heart beats. Potassium levels that are too high or too low can increase the risk of an abnormal heartbeat. Low potassium levels are also associated with muscle weakness.
Chloride
Chloride, like sodium, helps maintain a balance of fluids in the body. If there's a large loss of chloride, the blood may become more acidic and prevent certain chemical reactions from occurring in the body that are necessary it to keep working properly.
Bicarbonate
Bicarbonate prevents the body's tissues from getting too much or too little acid. The kidney and lungs balance the levels of bicarbonate in the body. So if bicarbonate levels are too high or low, it might indicate a problem with those organs.
NORMAL VALUES
serum or plasma sodium: 135145 mmol/l; alert levels: less than 120 mmol/l and greater than 160 mmol/l serum potassium: 3.65.4 mmol/l (plasma, 3.65.0 mmol/l); alert levels: less than 3.0 mmol/l and greater than 6.0 mmol/l serum or plasma chloride: 98108 mmol/l sweat chloride: 460 mmol/l serum or plasma bicarbonate: 1824 mmol/l (as total carbon dioxide, 2226 mmol/l); alert levels: less than 10 mmol/l and greater than 40 mmol/l serum calcium: 8.510.5 mg/dl (2.02.5 mmol/l); alert levels: less than 6.0 mg/dl and greater than 13.0 mg/dl ionized calcium: 1.01.3 mmol/l serum inorganic phosphorus: 2.34.7 mg/dl (children, 4.07.0 mg/dl); alert level: less than 1.0 mg/dl serum magnesium: 1.83.0 mg/dl (1.22.0 meq/l or 0.51.0 mmol/l) ionized magnesium: 0.530.67 mmol/l
An ABG test uses blood drawn from an artery, where the oxygen and carbon dioxide levels can be measured before they enter body tissues. Usually taken by specialty nurses, Respiratory Therapists Take specimens of blood from radial, brachial, femoral arteries
An ABG measures:
Partial pressure of oxygen (PaO2). This measures the pressure of oxygen dissolved in the blood and how well oxygen is able to move from the airspace of the lungs into the blood. Partial pressure of carbon dioxide (PaCO2). This measures how much carbon dioxide is dissolved in the blood and how well carbon dioxide is able to move out of the body.
pH. The pH measures hydrogen ions (H+) in blood. Bicarbonate (HCO3). Bicarbonate is a chemical (buffer) that keeps the pH of blood from becoming too acidic or too basic. Oxygen content (O2CT) and oxygen saturation (O2Sat) values. O2 content measures the amount of oxygen in the blood. Oxygen saturation measures how much of the hemoglobin in the red blood cells is carrying oxygen (O2).
Normal Values
pH 7.35 (7.4) to 7.45
PaCO2 35 (40) to 45 mm Hg
HCO3 22 (24) to 26 mEq/L (assumed average values for ABG interpretation) PaO2 80 to 100 mm Hg Oxygen saturation >94%
Nursing alert
Avoid suctioning prior to drawing of blood Place specimen on ice Apply firm pressure to the puncture site for 5-10 minutes Assess the puncture site for bleeding or hematoma
Coagulation Studies
Blood coagulation test is done to determine presence of illness. Before you have surgery your doctor may order blood tests to determine how quickly your blood clots. This group of tests is known as a coagulation study, individually these tests are commonly referred to as a PT (Prothrombin Time), PTT(Partial Thromboplastin Time), and INR (International Normalized Ratio).
During some surgeries it is important that the blood not clot as quickly as normal, and medications may be given to slow the clotting time. Drugs commonly used to slow clotting have a variety of names, but Heparin, Coumadin, Lovenox and Warfarin are among the most common. In other cases, the patient may not clot quickly enough, and steps may be taken to make the blood to clot more quickly.
Common causes of a prolonged PT include vitamin K deficiency, hormones drugs including hormone replacements and oral contraceptives, disseminated intravascular coagulation (a serious clotting problem that requires immediate intervention), liver disease, and the use of the anti-coagulant drug warfarin. Additionally, the PT result can be altered by a diet high in vitamin K, liver, green tea, dark green vegetables and soybeans.
Extended PTT times can be a result of anticoagulation therapy, liver problems, lupus and other diseases that result in poor clotting.
Sources:
The normal range for one lab would be different than a normal value from another lab, creating problems for patients who were being treated in several locations. In order to standardize the results between labs, the INR was created. The INR result should be the same, regardless of the location where the tests are performed.
Nursing Alerts
PTT critical level 175 seconds PT critical level 24 seconds Clotting Time 12 minutes If pt is taking anticoagulant, when the blood level value exceeds the critical value withold the next dose For pt taking heparin monitor PTT For pt taking coumadin monitor PT
BLOOD CHEMISTRY
There are a number of urine tests that can be used to assess kidney function. A simple, inexpensive screening testa routine urinalysis is often the first test conducted if kidney problems are suspected. If results indicate a possibility of disease or impaired kidney function, one or more of the following additional tests is usually performed to pinpoint the cause and the level of
The test is performed on a timed urine specimena cumulative sample collected over a two to 24-hour period. Determination of the blood creatinine level is also required to calculate the urine clearance.
The test may be done on a urine sample collected first thing in the morning, on multiple timed samples, or on a cumulative sample collected over a 24hour period. The patient will typically be prescribed a high-protein diet for several days before the test and be asked to drink no fluids the night before the test.
Creatinine clearance. For a 24-hour urine collection, normal results are 90 mL/min139 mL/min for adult males younger than 40, and 80125 mL/min for adult females younger than 40. For people over 40, values decrease by 6.5 mL/min for each decade of life. Urine osmolality. With restricted fluid intake (concentration testing), osmolality should be greater than 800 mOsm/kg of water. With increased fluid intake (dilution testing), osmolality should be less than 100 mOSm/kg in at least one of the specimens collected. A 24-hour urine osmolality should average 300900 mOsm/kg. A random urine osmolality should average 500800 mOsm/kg. Urine protein. A 24-hour urine collection should contain no more than 150 mg of protein. Urine sodium. A 24-hour urine sodium should be within 75 200 mmol/day.
Normal Value
Normal Value
Normal urine is transparent. Turbid (cloudy) urine may be caused by either normal or abnormal processes. Normal conditions giving rise to turbid urine include precipitation of crystals, mucus, or vaginal discharge. Abnormal causes of turbidity include the presence of blood cells, yeast, and bacteria.
Glucose: negative (quantitative less than 130 mg/day or 30 mg/dL). Bilirubin: negative (quantitative less than 0.02 mg/dL). Ketones: negative (quantitative 0.53.0 mg/dL). pH: 5.08.0. Protein: negative (quantitative 15150 mg/day, less than 10 mg/dL). Blood: negative. Nitrite: negative.
Specific gravity: 1.0031.025. Urobilinogen: 02 Ehrlich units (quantitative 0.31.0 Ehrlich units). Leukocyte esterase: negative. Red blood cells: 02 per high power field. White blood cells: 05 per high power field (010 per high power field for some standardized systems).
Nursing Alerts
First voided urine for urinalysis Use clean container Decreased sp. gr. Diabetes insipidus Increased sp. gr. Diabetes mellitus (+) Protein: nephrotic syndrome (+) Glucose: D.M. infection
Creatinine test
This test measures blood levels of creatinine, a by-product of muscle energy metabolism that, similar to urea, is filtered from the blood by the kidneys and excreted into the urine. Creatinine is affected very little by liver function, an elevated blood creatinine level is a more sensitive indicator of impaired kidney function than the BUN.
N.V. 0.6 to 1.3 mg/dl Nursing considerations: instruct the client to avoid excessive for 8 hours and excessive red meat intake for 24 hrs before the test.
Cardiac markers
Cardiac marker tests identify blood chemicals associated with myocardial infarction (MI), commonly known as a heart attack. The myocardium is the middle layer of the heart wall composed of heart muscle. Infarction is tissue death caused by an interruption in the blood supply to an area.
Cardiac markers help physicians to assess acute coronary syndromes and to identify and manage high-risk patients. Creatine kinase-MB (CK-MB), myoglobin, homocysteine, C-reactive protein (CRP), troponin T (cTnT), and troponin I (cTnI) are all used for assessment of the suspected acute myocardial infarction. CK-MB, cTnT, and cTnI may also be used to identify and manage high-risk patients.
Creatine Kinase
Creatine kinase is an enzyme found in muscle and brain tissue that reflects tissue catabolism resulting from cell trauma. The test for CK is performed to detect myocardial or skeletal muscle damage or central nervous system damage.
Isoenzymes includes:
CK MB found mainly in the cardiac muscle CK BB is found mainly in brain tissue CK MM found mainly in the skeletal muscles
Normal Value
Total CK: Reference value is 38174 units/L for men and 26140 units/L for women. The values begin to rise within four to six hours and peak at 24 hours. Values return to normal within three to four days.
CK-MB: Reference value is 1013 units/L. The values begin to rise within three to four hours and peak at 10 24 hours. Values return to normal within two to four days .
NURSING CONSIDERATIONS
Invasive procedures and IM injections may elevate CK levels falsely. If the test is to evaluate skeletal muscle, instruct the client to avoid strenuous activities for 24 hrs before the test
Myoglobin
is a protein found in both skeletal and myocardial muscle. It is released rapidly after tissue injury and may be elevated as early as one hour after myocardial injury, though it may also be elevated due to skeletal muscle trauma. However, if myoglobin values do not rise within three to four hours after a person shows acute symptoms, it is highly unlikely that he or she had an MI.
Normal Value
Myoglobin: Reference value is less than 110 nanograms/mL. The values begin to rise within one to two hours and peak at four to eight hours. Values return to normal within 1224 hours.
Troponins
A regulatory protein found in the striated muscle (skeletal and myocardial) Increased amounts of troponins are released into the blood stream when an infarction causes damage to the myocardium
Cardiac troponin T (cTnT) and cardiac troponin I (cTnI) are the newest additions to the list of cardiac markers. Cardiac troponins are specific to heart muscle. They have enabled the development of assays (tests) that can detect heart muscle injury with great sensitivity and specificity
While these markers have been used mainly to aid in the diagnosis of chest-pain patients with nondiagnostic electrocardiograms, they are also used as prognostic indicators of a MI. According to the American Heart Association, "Several studies have identified a measurable relationship between cardiac troponin levels and long-term outcome after an episode of chest discomfort. They suggest that these tests may be particularly useful to evaluate levels of risk. In other words, it's possible that the results of a troponin test could be used to identify people at either low risk or high risk for later, serious heart problems."
Troponin I: value is usually less than 0.6 ng/ml; greater than 1.5 ng/ml is consistent with MI Troponin T: greater than 0.2 ng/ml is consistent with MI No need for fasting
LDH begins to rise about 24 hrs after MI and peak in 48 72 hours; returns to normsl within 7 14 days Lactate dehydrogenase 140-280 units/L Isoenzymes
LDH 1 14 - 26% LDH-2 29 39%
Nursing Considerations The LDH isoenzymes should be interpreted in view of the clinical findings Testing should be repeated on 3 consecutive days.
Several recent studies have demonstrated that CRP levels are useful in predicting the risk for a thrombotic event (such as a blood clot causing MI). These studies suggest that a high-sensitivity assay for CRP be used that is capable of measuring the very low level normally found in serum (0.12.5 mg/L). Heart patients who have persistent CRP levels between 4 and 10 mg/L, with clinical evidence of low-grade inflammation, should be considered to be at increased risk for thrombosis.
Normal Value
C-reactive protein: According to the U.S. Food and Drug Administration, in healthy people, reference values are below 5 mg/dL; in various diseases, this threshold is often exceeded within four to eight hours after an acute inflammatory event, with CRP values reaching approximately 20500 mg/dL.
Homocysteine
Homocysteine is an amino acid. According to the American Heart Association, studies have shown that too much homocysteine in the blood is related to a higher risk of coronary heart disease, stroke, and peripheral vascular disease; and that it may also have an effect on atherosclerosis. High levels of homocysteine are the result of a lack of certain B vitamins, inheritance, or dietary excess and have been implicated in vascular-wall injury.
It is believed that laboratory testing for plasma homocysteine levels can improve the assessment of risk, particularly in patients with a personal or family history of cardiovascular disease, but in whom the well-established risk factors (smoking, high blood cholesterol, high blood pressure, physical inactivity, obesity, and diabetes) do not exist.
Normal value
The normal fasting level for plasma is 515 micromol/L. Moderate, intermediate, and severe hyperhomocysteinemia refer to concentrations between 16 and 30, between 31 and 100, and less than 100 micromol/L, respectively.
Liver function tests may be done for a variety of reasons. Liver function tests can help screen for liver viruses such as hepatitis. They can also help monitor the progression of a disease like viral or alcoholic hepatitis and determine how well a treatment is working. Liver function tests can even measure the severity of a disease, particularly if you have cirrhosis an irreversible scarring of the liver.
LFTs include measurements of albumin, various liver enzymes (ALT, AST, GGT and ALP), bilirubin, prothrombin time, cholesterol and total protein. All of these tests can be performed at the same time.
The normal range of ALT levels is between 5 IU/L to 60 IU/L (International Units per Liter). ALT levels in people with HCV often rise and fall over time, so additional testing such as HCV RNA, HCV genotyping and a liver biopsy may be needed to help determine the cause and extent of liver damage.
Aspartate aminotransferase (AST). This enzyme, which plays a role in processing proteins, is found in the liver, heart, muscles, and kidneys. When the liver is injured or inflamed, levels of AST in the blood usually rise. Also known as serum glutamic oxaloacetic transaminase
the bloodstream are 5 IU/L to 43 IU/L. Like ALT levels, AST levels in people with HCV often vary over time and can't be used to forecast disease progression or specifically measure liver damage.
GGT and ALP are also called cholestatic liver enzymes. Chloestasis is a term used for partial or full blockage of the bile ducts. Bile ducts bring bile from the liver into the gallbladder and the intestines. Bile is a green fluid produced in liver cells. Bile helps the body to break down fat, process cholesterol and get rid of toxins. If the bile duct is inflamed or damaged, GGT and ALP can get backed up and spill out from the liver into the bloodstream. ALP metabolizes phosphorus and brings energy to the body. GGT brings oxygen to tissues.
Scarring of the bile ducts (called primary biliary cirrhosis), Fatty liver (steatosis), sAlcoholic liver disease, Liver inflammation from medications and certain herbs, Liver tumors, Gallstones or gall bladder problems.
ALP (also called alkaline phosphatase) is found in the bones, intestines, kidneys and placenta as well as the liver. Abnormally high ALP can have many causes other than liver damage, including: bone disease, congestive heart failure, and hyperthyroidism. A rise in ALP levels can indicate liver trouble if GGT levels are also elevated. The normal range of ALP is from 30 IU/L to 115 IU/L.
GGT (gamma-glutamyltranspeptidase) is found in the liver. Obesity, PBC, heavy drinking, fatty liver, and certain medications or herbs that are toxic to the liver can cause GGT levels to rise the normal range of GGT is from 5 IU/L to 80 IU/L.
Bilirubin is a yellow fluid produced in the liver when worn-out red blood cells are broken down. Bilirubin can leak out from the liver into the bloodstream if the liver is damaged. When bilirubin builds up, it can cause jaundice - a yellowing of the eyes and skin, dark urine and light colored feces
The causes of abnormal bilirubin levels include: Viral hepatitis, Blocked bile ducts, Other liver diseases, Liver scarring (cirrhosis)
Total bilirubin testing measures the amount of bilirubin in the bloodstream. Normal total bilirubin levels range from .20mg/dl to 1.50 (milligrams per deciliter). Direct bilirubin testing measures bilirubin made in the liver. The normal level of direct bilirubin range from .00 to .03 mg/dl
Total protein testing (also called TP or serum total protein) measures the amount of proteins in the bloodstream. Many different things can cause abnormally high or low protein levels. A doctor may order total protein testing to help diagnose kidney or liver disease, blood cancer, malnutrition or abnormal body swelling. Normal protein levels in the bloodstream range from 6.5 to 8.2 grams per deciliter
Albumin is a protein made in the liver. If the liver is badly damaged, it can no longer produce albumin. Albumin maintains the amount of blood in the veins and arteries. When albumin levels become very low, fluid can leak out from the blood vessels into nearby tissues, causing swelling in the feet and ankles. Very low levels of albumin may be a sign of liver damage. The normal albumin range is from 3.9 grams/ deciliter to 5.0 grams/deciliter
Prothrombin (also called factor II) is a protein that helps to clot blood. Prothrombin is made in the liver. A prothrombin time test measures how much time it takes for a person's blood to clot. The normal time needed for blood to clot is between 10 and 15 seconds.
Nursing considerations
Although the hepatic function panel can be done without any preparation, it's more accurate when performed after fasting. Your child may be asked to stop eating and drinking for 10 to 12 hours before this blood test. Draw from extremity that does not have IV infusion on it Medications that are hepatotoxic should not be given within 12 hours prior to test
Instruct patient to eat a diet low in yellow food for 3 to 4 days before the blood is drawn (bilirubin) Transport the specimen to laboratory immediately
Lipoproteins Profile
Lipoproteins are the "packages" in which cholesterol and triglycerides travel throughout the body. Measuring the amount of cholesterol carried by each type of lipoprotein helps determine a person's risk for cardiovascular disease (disease that affects the heart and blood vessels, also called CVD).
Cholesterol and triglycerides are fat-like substances called lipids. Cholesterol is used to build cell membranes and hormones. The body makes cholesterol and gets it from food. Triglycerides provide a major source of energy to the body tissues. Both cholesterol and triglycerides are vital to body function, but an excess of either one, especially cholesterol, puts a person at risk of cardiovascular disease.
Because cholesterol and triglycerides can't dissolve in watery liquid, they must be transported by something that can dissolve in blood serum. Lipoproteins contain cholesterol and triglycerides at the core and an outer layer of protein, called apolipoprotein.
LDL carries cholesterol in the blood and deposits it in body tissues and in the walls of blood vessels, a condition known as atherosclerosis. The amount of LDL in a person's blood is directly related to his or her risk of cardiovascular disease. The higher the LDL level, the greater the risk. LDL is the lipoprotein class most used to trigger and monitor cholesterol lowering therapy.
HDL is often called "good" cholesterol. HDL removes excess cholesterol from tissues and vessel walls and carries it to the liver, where it is removed from the blood and discarded. The amount of HDL in a person's blood is inversely related to his or her risk of cardiovascular disease. The lower the HDL level, the greater the risk; the higher the level, the lower the risk. The smallest lipoprotein, it contains 20-30% of a person's total cholesterol and can be separated into two major subclasses.
239 mg/dL, your doctor will evaluate your levels of LDL (bad) cholesterol, HDL (good) cholesterol and triglycerides. It's possible to have borderline-high total cholesterol numbers with normal levels of LDL (bad) cholesterol balanced by high HDL (good) cholesterol. Make lifestyle changes, including eating a hearthealthy diet, getting regular physical activity and avoiding tobacco smoke. Depending on your LDL (bad) cholesterol levels and your other risk factors, you may also need medication.
240 mg/dL and over: High Risk People who have a total cholesterol level of 240 mg/dL or more typically have twice the risk of coronary heart disease as people whose cholesterol level is desirable (200 mg/dL). If your test didnt show your LDL cholesterol, HDL cholesterol and triglycerides, your doctor should order a fasting profile. You need cholesterol-regulating medication, make lifestyle changes, including eating a hearthealthy diet, getting regular physical activity and avoiding tobacco smoke.
Smoking, being overweight and being sedentary can all result in lower HDL cholesterol. To raise your HDL level, avoid tobacco smoke, maintain a healthy weight and get at least 3060 minutes of physical activity more days than not. People with high blood triglycerides usually also have lower HDL cholesterol and a higher risk of heart attack and stroke. Progesterone, anabolic steroids and male sex hormones (testosterone) also lower HDL cholesterol levels. Female sex hormones raise HDL cholesterol levels
The lower your LDL cholesterol, the lower your risk of heart attack and stroke. In fact, it's a better gauge of risk than total blood cholesterol.
Your triglyceride level will fall into one of these categories: Normal: less than 150 mg/dL Borderline-High: 150199 mg/dL High: 200499 mg/dL Very High: 500 mg/dL
Many people have high triglyceride levels due to being overweight/obese, physical inactivity, cigarette smoking, excess alcohol consumption and/or a diet very high in carbohydrates (60 percent of more of calories). High triglycerides are a lifestyle-related risk factor; however, underlying diseases or genetic disorders can be the cause. The main therapy to reduce triglyceride levels is to change your lifestyle. Sometimes, medication is needed in
Nursing considerations
The patient must fast for 12 hours before the test, eating nothing and drinking only water. The person should not have alcohol for 24 hours before the test. There should be a stable diet (free of high cholesterol foods the night before the test No illnesses occurring in the preceding two weeks.
Glucose Studies
A blood glucose test measures the amount of a type of sugar, called glucose, in your blood. Glucose comes from carbohydrate foods. It is the main source of energy used by the body. Insulin is a hormone that helps your body's cells use the glucose. Insulin is produced in the pancreas and released into the blood when the amount of glucose in the blood rises.
Normally, our blood glucose levels increase slightly after we eat. This increase causes our pancreas to release insulin so that our blood glucose levels do not get too high. Blood glucose levels that remain high over time can damage your eyes, kidneys, nerves, and blood vessels.
Different types of blood glucose tests Fasting blood sugar (FBS) measures blood glucose after you have not eaten for at least 8 hours. It often is the first test done to check for diabetes.
Normal Values
N.V. 70 110 mg/dL If your fasting blood glucose level measures in the range of 100 mg/dL (5.5 mmol/L) to 125 mg/dL (6.9 mmol/L), you are considered to have prediabetes (impaired fasting glucose), and you have an increased chance of getting diabetes. A fasting glucose level below 40 mg/dL (2.2 mmol/L) in women or below 50 mg/dL (2.8 mmol/L) in men that is accompanied by symptoms of hypoglycemia may mean you have an insulinoma, a tumor that produces abnormally high amounts of insulin.
Nursing Consideration
For a fasting blood sugar test, do not eat or drink anything other than water for at least 8 hours before the blood sample is taken. If you have diabetes, you may be asked to wait until you have had your blood tested before taking your morning dose of insulin or diabetes medication.
2-hour postprandial blood sugar measures blood glucose exactly 2 hours after you eat a meal. N.V. - 70145 mg/dL (less than 7.9 mmol/L)
Nursing Consideration
For a 2-hour postprandial test, eat a meal exactly 2 hours before the blood sample is taken.
Random blood sugar (RBS) measures blood glucose regardless of when you last ate. Several random measurements may be taken throughout the day. Random testing is useful because glucose levels in healthy people do not vary widely throughout the day. Blood glucose levels that vary widely may indicate a problem. This test is also called a casual blood glucose test.
N.V. - 70125 mg/dL (less than 7.0 mmol/L) No special preparation is required before having a random blood sugar test.
Oral glucose tolerance test is used to diagnose prediabetes and diabetes. An oral glucose tolerance test is a series of blood glucose measurements taken after you drink a sweet liquid that contains glucose. This test is commonly used to diagnose diabetes that occurs during pregnancy (gestational diabetes).
Instruct the client to eat a high carbohydrate (200 300 mg/dL) diet for 3 days before the test. Instruct the client to avoid alcohol, coffee, and smoking for 36 hours before the test. Instruct the client to fast for 10 to 16 hours before the test Instruct the client to avoid strenuous exercise for 8 hours before and after the test. Withhold morning insulin or oral hypoglycemic meds.
Nursing Considerations
Instruct the client that the test will take 3 to 5 hours, requires intravenous or oral administration of glucose, and multiple blood samples.
Glycosylated hemoglobin
Glycosylated hemoglobin is blood glucose bound to hemoglobin. Hemoglobin A1 c (Glycosylated hemoglobin A) is a reflection of how well blood glucose levels have been controlled for up to 4 months
Normal Values
Values are expressed as a percentage of the total hemoglobin
Diabetic with good control: 7.5% or less Diabetic with fair control: 7.6% to 8.9% Diabetic with poor control: 9% or greater
Nursing Consideration
Fasting is not required before the test.
The American Diabetes Association (ADA) criteria for diagnosing diabetes are met when any of the following results have been repeated on at least two different days:
A fasting blood glucose level is 126 mg/dL (7.0 mmol/L) or higher. A 2-hour oral glucose tolerance test result is 200 mg/dL (11.1 mmol/L) or higher. For more information, see the medical test Oral Glucose Tolerance Test. Symptoms of diabetes are present and a random blood glucose test is 200 mg/dL (11.1 mmol/L) or higher. Symptoms of diabetes include increased thirst and frequent urination (especially at night), unexplained increase in appetite, unexplained weight loss, fatigue, erection problems, blurred vision, and tingling or numbness in the hands or feet.